Analysis the Function of R2R3 MYB Transcription Factor RhMYB113c on Regulating Anthocyanin Synthesis in Rosa hybrida

doi:10.16420/j.issn.0513-353x.2021-0630

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1957-1966.doi: 10.16420/j.issn.0513-353x.2021-0630

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Analysis the Function of R2R3 MYB Transcription Factor RhMYB113c on Regulating Anthocyanin Synthesis in Rosa hybrida

LI Maofu¹^,²^,³, YANG Yuan¹^,²^,⁴, WANG Hua¹^,²^,³, FAN Youwei¹^,²^,³, SUN Pei¹^,²^,³, JIN Wanmei¹^,²^,³^,^*()

¹Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China
²Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（North China）,Ministry of Agriculture and Rural Affairs,Beijing 100093,China
³Beijing Engineering Research Center of Functional Floriculture,Beijing 100093,China
⁴Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China

Received:2021-07-02 Revised:2021-09-10 Online:2022-09-25 Published:2022-10-08
Contact: JIN Wanmei E-mail:jwm0809@163.com

Abstract

Abstract:

An R2R3 MYB gene,named RhMYB113c,was cloned from cDNA of the petal in Rosa hybrida‘Carola’. The open reading frame of RhMYB113c was 699 base pair,encoding 233 amino acids. The multiple sequence alignment analysis showed that RhMYB113c contained R2 and R3 domains. Phylogenetic tree results indicated that RhMYB113c was closely related to anthocyanin synthesis activator AtMYB114,AtMYB113,AtMYB75,and AtMYB90. Tissue specific expression showed that RhMYB113c was mainly expressed in petals and styles in‘Carola’,but not in all tissues of white flower variety ‘White Satin’. Gene expression at different flower development stages indicated that RhMYB113c was not expressed in‘Carola’uncolored petals,highly expressed in full flowering stage,but not in all stage of white flower varieties‘White Satin’. Yeast two hybrid and tobacco transient expression experiments suggested that RhMYB113c may regulate anthocyanin synthesis and determine the color of rose petals.

Key words: Rosa, petal, RhMYB113c, anthocyanin synthesis

CLC Number:

S685.12

LI Maofu, YANG Yuan, WANG Hua, FAN Youwei, SUN Pei, JIN Wanmei. Analysis the Function of R2R3 MYB Transcription Factor RhMYB113c on Regulating Anthocyanin Synthesis in Rosa hybrida[J]. Acta Horticulturae Sinica, 2022, 49(9): 1957-1966.

Figures/Tables 7

References 34

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目的 Purpose	引物名称 Primer name	引物序列（5′-3′） Primer sequences
基因克隆 Gene clone	RhMYB113c	F：CAACGTCATTAACTGTGGGATC R：GCCGTGAGCAGTGGCTTTC
瞬时转化 Transient expression	RhMYB113c	F：GAAATTtctagaATGGAGGACCAGTCGGGTTTGAG R：GAAATTgagctcTCATTATCGATCTAAGAATGTCATCC
瞬时转化 Transient expression	RcbHLH	F：GAAATTtcgcgaATGGCTACACCGCCACCGAGTAGTAGC R：GAAATTgagctcTCATTATCGATCTAAGAATGTCATCC
酵母双杂交 Yeast two-hybrid assay	RhMYB113c:AD,RhMYB113c:BD	F：GAAATTcatatgATGGAGGACCAGTCGGGTTTGAG R：GAAATTgaattcTCATTATCGATCTAAGAATGTCATCC
实时荧光定量PCR RT-qPCR	RhMYB113c	F：ACCAAGCGGCGTCGGGACAAAC R：CCCGTCAAACAGAGTGAACTGGTCG
实时荧光定量PCR RT-qPCR	Actin	F：GGCTGTTCTCTCTCTGTATGC R：TTCTGGGCACCTGAATCTC

目的 Purpose	引物名称 Primer name	引物序列（5′-3′） Primer sequences
基因克隆 Gene clone	RhMYB113c	F：CAACGTCATTAACTGTGGGATC R：GCCGTGAGCAGTGGCTTTC
瞬时转化 Transient expression	RhMYB113c	F：GAAATTtctagaATGGAGGACCAGTCGGGTTTGAG R：GAAATTgagctcTCATTATCGATCTAAGAATGTCATCC
瞬时转化 Transient expression	RcbHLH	F：GAAATTtcgcgaATGGCTACACCGCCACCGAGTAGTAGC R：GAAATTgagctcTCATTATCGATCTAAGAATGTCATCC
酵母双杂交 Yeast two-hybrid assay	RhMYB113c:AD,RhMYB113c:BD	F：GAAATTcatatgATGGAGGACCAGTCGGGTTTGAG R：GAAATTgaattcTCATTATCGATCTAAGAATGTCATCC
实时荧光定量PCR RT-qPCR	RhMYB113c	F：ACCAAGCGGCGTCGGGACAAAC R：CCCGTCAAACAGAGTGAACTGGTCG
实时荧光定量PCR RT-qPCR	Actin	F：GGCTGTTCTCTCTCTGTATGC R：TTCTGGGCACCTGAATCTC

Analysis the Function of R2R3 MYB Transcription Factor RhMYB113c on Regulating Anthocyanin Synthesis in Rosa hybrida

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