Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1935-1944.doi: 10.16420/j.issn.0513-353x.2021-0638
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HUANG Ling, HU Xianmei, LIANG Zehui, WANG Yanping, CHAN Zhulong*(), XIANG Lin*(
)
Received:
2021-08-16
Revised:
2021-10-13
Online:
2022-09-25
Published:
2022-10-08
Contact:
CHAN Zhulong,XIANG Lin
E-mail:zlchan@mail.hzau.edu.cn;xianglin@mail.hzau.edu.cn
CLC Number:
HUANG Ling, HU Xianmei, LIANG Zehui, WANG Yanping, CHAN Zhulong, XIANG Lin. Cloning and Function Identification of Anthocyanidin Synthase Gene TgANS in Tulipa gesneriana[J]. Acta Horticulturae Sinica, 2022, 49(9): 1935-1944.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0638
引物 Primer | 正向引物序列(5′-3′) Forward sequence | 反向引物序列(5′-3′) Reverse sequence |
---|---|---|
TgANS | CGCTCGAGATCCTCAGCAAT | GTCCGGGGAGGAAACTTAGC |
TgUBQ10 | GTCGCACGTTGGCTGATTAC | GGGCTTAAAGACCACCACGA |
NtCHS | AAGCAAGAGAAACTAAAGGCTACAAG | AAATCCAAAAAGCACACCCCAT |
NtCHI | CGGGTGCCTCCATTCTTTTTACT | CCTGACACTCTTTCGGCGATACTAC |
NtF3H | CCAGACAAACCAGATGGATGGATAG | CAAGGGTAAGGTCGGGCTGTG |
NtF3’H | TGGCTATTTCATTCCAAAAGGCTCA | CTTCAAAGTCATTTCCTCGCACATC |
NtDFR | GCAGTTGCTTCCCTTTTCTACC | TTCCCCATTGGTTGACTTTCC |
NtFLS | CTTGAAGGGAAAAGGGGTTGG | CGCAACTTCTCGCAGCCTCT |
NtEF1α | TGGTTGTGACTTTTGGTCCCA | ACAAACCCACGCTTGAGATCC |
Table 1 The primers used for qRT-PCR
引物 Primer | 正向引物序列(5′-3′) Forward sequence | 反向引物序列(5′-3′) Reverse sequence |
---|---|---|
TgANS | CGCTCGAGATCCTCAGCAAT | GTCCGGGGAGGAAACTTAGC |
TgUBQ10 | GTCGCACGTTGGCTGATTAC | GGGCTTAAAGACCACCACGA |
NtCHS | AAGCAAGAGAAACTAAAGGCTACAAG | AAATCCAAAAAGCACACCCCAT |
NtCHI | CGGGTGCCTCCATTCTTTTTACT | CCTGACACTCTTTCGGCGATACTAC |
NtF3H | CCAGACAAACCAGATGGATGGATAG | CAAGGGTAAGGTCGGGCTGTG |
NtF3’H | TGGCTATTTCATTCCAAAAGGCTCA | CTTCAAAGTCATTTCCTCGCACATC |
NtDFR | GCAGTTGCTTCCCTTTTCTACC | TTCCCCATTGGTTGACTTTCC |
NtFLS | CTTGAAGGGAAAAGGGGTTGG | CGCAACTTCTCGCAGCCTCT |
NtEF1α | TGGTTGTGACTTTTGGTCCCA | ACAAACCCACGCTTGAGATCC |
Fig. 1 Analysis of anthocyanins content and relative expression levels of TgANS in tulip flower development The values indicates averages of three independent replicates and SEs. P < 0.05,Tukey’s method.
Fig. 2 Alignment of the deduced amino acid sequences of ANS from other plant species TgANS sequence was aligned with ANS from Tulipa fosteriana(TfANS,AGJ50591);Lilium cernuum(LcANS,AGC92011);L. hybrida(LhANS,ALA55544);L. speciosum(LsANS,BAS69334);Iris sanguinea(IsANS,QCI56004);Phoenix dactylifera(PdANS,XP_008783582). The black underline shows the conserved domain 2OG-Fe II_Oxy. * and # indicate conserved residues which are required for ferrous-iron coordination,and 2-oxoglutarat binding,respectively.
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