Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (9): 1907-1921.doi: 10.16420/j.issn.0513-353x.2021-0807
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TAN Shanshan1, QIU Liang1,3, DUAN Aoqi1, SHU Sheng1,2, ZENG Xiaoping4, ZHU Weimin5, JIA Min1, YAN Jun5, LIU Yanhua1, LIU Hui1, XIONG Aisheng1()
Received:
2022-01-04
Revised:
2022-06-10
Online:
2022-09-25
Published:
2022-10-08
CLC Number:
TAN Shanshan, QIU Liang, DUAN Aoqi, SHU Sheng, ZENG Xiaoping, ZHU Weimin, JIA Min, YAN Jun, LIU Yanhua, LIU Hui, XIONG Aisheng. Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery[J]. Acta Horticulturae Sinica, 2022, 49(9): 1907-1921.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0807
基因Gene | 序列(5′-3′)Sequence |
---|---|
脱植基叶绿素a氧化酶基因 CAO | F:TTGGTGAATGATAGGCTGTT;R:GGATGGTAAGGTTGGACTG |
叶绿素b还原酶基因 NOL | F:TCTCTTCTTCGCATCATCTT;R:TTGAGCCAGCATTGTTGA |
7-羟甲基叶绿素a还原酶基因 HCAR | F:CCAACTTAGCCTTATCATCTG;R:TGCCACAACATCCACAAT |
H亚基镁螯合酶基因 ChlH | F:GAATAGTAGCCGCATAGGAT;R:ACAAGAAGGTAGCCATCAC |
镁原卟啉氧甲基转移酶基因 ChlM | F:TATCAGTCTCCGCCATTTTCCCTCA;R:GTGTCCCCGTATATTTTCTTCCACC |
镁原卟啉Ⅸ单酯环化酶基因 MPE | F:ATGATACACTGAAGCGGATT;R:CCACTAATAAGCAGCAACAA |
脱镁叶绿素酶基因 PPH | F:CAAGCAGAGGCATCACAA;R:CCGTATAATGGAGACAACAAG |
脱镁叶绿酸氧化酶基因 PAO | F:ATGTGGAGGAACGGAAGA;R:GTAGGCATCTAACGGAAGG |
芹菜内参基因 Agactin | F:CTTCCTGCCATATATGATTGG;R:GCCAGCACCTCGATCTTCATG |
Table 1 Primer sequences for RT-qPCR
基因Gene | 序列(5′-3′)Sequence |
---|---|
脱植基叶绿素a氧化酶基因 CAO | F:TTGGTGAATGATAGGCTGTT;R:GGATGGTAAGGTTGGACTG |
叶绿素b还原酶基因 NOL | F:TCTCTTCTTCGCATCATCTT;R:TTGAGCCAGCATTGTTGA |
7-羟甲基叶绿素a还原酶基因 HCAR | F:CCAACTTAGCCTTATCATCTG;R:TGCCACAACATCCACAAT |
H亚基镁螯合酶基因 ChlH | F:GAATAGTAGCCGCATAGGAT;R:ACAAGAAGGTAGCCATCAC |
镁原卟啉氧甲基转移酶基因 ChlM | F:TATCAGTCTCCGCCATTTTCCCTCA;R:GTGTCCCCGTATATTTTCTTCCACC |
镁原卟啉Ⅸ单酯环化酶基因 MPE | F:ATGATACACTGAAGCGGATT;R:CCACTAATAAGCAGCAACAA |
脱镁叶绿素酶基因 PPH | F:CAAGCAGAGGCATCACAA;R:CCGTATAATGGAGACAACAAG |
脱镁叶绿酸氧化酶基因 PAO | F:ATGTGGAGGAACGGAAGA;R:GTAGGCATCTAACGGAAGG |
芹菜内参基因 Agactin | F:CTTCCTGCCATATATGATTGG;R:GCCAGCACCTCGATCTTCATG |
品种 Cultivar | NaClO/% | 发芽势/% Germination potential | 发芽率/% Germination rate | 发芽指数 Germination index | 活力指数 Vigor index | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | ||
六合黄心芹 Liuhe Huangxinqin | 0 | 65.33 a | 56.00 ab | 82.67 a | 70.44 ab | 6.82 a | 10.08 ab | 144.90 a | 286.10 b | |||
5 | 77.33 a | 69.33 a | 83.33 a | 81.78 a | 7.06 a | 11.89 a | 173.51 a | 371.48 a | ||||
10 | 62.00 a | 47.11 bc | 76.00 ab | 64.89 abc | 6.38 ab | 9.13 bc | 149.86 a | 224.99 bc | ||||
20 | 62.00 a | 49.11 b | 73.33 ab | 62.00 bc | 6.09 ab | 8.88 bc | 156.52 a | 211.95 c | ||||
40 | 42.67 b | 31.11 c | 62.00 b | 51.56 c | 4.92 b | 6.78 c | 79.69 b | 110.52 d | ||||
赛雪 Saixue | 0 | 59.67 b | 61.33 a | 76.67 b | 73.11 ab | 6.14 b | 10.60 ab | 178.35 b | 302.60 b | |||
5 | 75.87 a | 64.03 a | 91.33 a | 78.33 a | 7.54 a | 11.26 a | 235.33 a | 396.21 a | ||||
10 | 58.00 b | 50.22 b | 76.00 bc | 68.44 ab | 6.11 bc | 9.68 bc | 159.70 b | 310.39 b | ||||
20 | 61.33 b | 49.33 b | 72.67 bc | 67.33 b | 5.95 bc | 9.56 bc | 182.52 b | 209.57 c | ||||
40 | 47.00 c | 41.22 c | 62.00 c | 64.67 b | 4.96 c | 8.95 c | 79.97 c | 223.81 c |
Table 2 Germination of celery seeds under different concentrations of NaClO treatments
品种 Cultivar | NaClO/% | 发芽势/% Germination potential | 发芽率/% Germination rate | 发芽指数 Germination index | 活力指数 Vigor index | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | ||
六合黄心芹 Liuhe Huangxinqin | 0 | 65.33 a | 56.00 ab | 82.67 a | 70.44 ab | 6.82 a | 10.08 ab | 144.90 a | 286.10 b | |||
5 | 77.33 a | 69.33 a | 83.33 a | 81.78 a | 7.06 a | 11.89 a | 173.51 a | 371.48 a | ||||
10 | 62.00 a | 47.11 bc | 76.00 ab | 64.89 abc | 6.38 ab | 9.13 bc | 149.86 a | 224.99 bc | ||||
20 | 62.00 a | 49.11 b | 73.33 ab | 62.00 bc | 6.09 ab | 8.88 bc | 156.52 a | 211.95 c | ||||
40 | 42.67 b | 31.11 c | 62.00 b | 51.56 c | 4.92 b | 6.78 c | 79.69 b | 110.52 d | ||||
赛雪 Saixue | 0 | 59.67 b | 61.33 a | 76.67 b | 73.11 ab | 6.14 b | 10.60 ab | 178.35 b | 302.60 b | |||
5 | 75.87 a | 64.03 a | 91.33 a | 78.33 a | 7.54 a | 11.26 a | 235.33 a | 396.21 a | ||||
10 | 58.00 b | 50.22 b | 76.00 bc | 68.44 ab | 6.11 bc | 9.68 bc | 159.70 b | 310.39 b | ||||
20 | 61.33 b | 49.33 b | 72.67 bc | 67.33 b | 5.95 bc | 9.56 bc | 182.52 b | 209.57 c | ||||
40 | 47.00 c | 41.22 c | 62.00 c | 64.67 b | 4.96 c | 8.95 c | 79.97 c | 223.81 c |
品种 Cultivar | NaClO/% | 根长/mm Root length | 下胚轴长/mm Hypocotyl length | 单株鲜质量/mg Fresh weight per plant | 单株干质量/mg Dry weight per plant | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | |||
六合黄心芹 Liuhe Huangxinqin | 0 | 21.25 ab | 28.39 ab | 9.59 bc | 13.38 b | 3.82 d | 4.65 c | 0.28 c | 0.35 ab | |||
5 | 24.57 a | 31.25 a | 10.26 b | 13.41 b | 5.29 b | 5.19 b | 0.33 a | 0.36 a | ||||
10 | 23.50 a | 24.64 b | 14.59 a | 10.61 b | 5.43 a | 5.27 a | 0.33 a | 0.36 a | ||||
20 | 25.71 a | 23.87 b | 8.11 c | 13.59 b | 5.43 a | 5.20 b | 0.30 b | 0.35 ab | ||||
40 | 16.21 b | 16.31 c | 8.17 c | 26.91 a | 4.14 c | 3.32 d | 0.29 bc | 0.22 c | ||||
赛雪Saixue | 0 | 29.03 a | 28.56 abc | 14.47 a | 10.35 b | 4.20 d | 5.99 c | 0.30 c | 0.46 b | |||
5 | 31.20 a | 35.19 a | 15.13 a | 14.43 b | 4.72 b | 6.57 b | 0.36 b | 0.53 a | ||||
10 | 26.15 ab | 32.05 ab | 11.51 b | 11.07 b | 4.80 a | 6.99 a | 0.47 a | 0.42 bc | ||||
20 | 30.69 a | 21.93 c | 11.41 b | 13.89 b | 4.62 c | 5.65 d | 0.36 b | 0.37 d | ||||
40 | 16.11 b | 25.02 bc | 8.73 c | 20.01 a | 4.61 c | 6.53 b | 0.30 c | 0.38 cd |
Table 3 Effects of the growth of 20-day-old seedlings under different concentrations of NaClO treatments
品种 Cultivar | NaClO/% | 根长/mm Root length | 下胚轴长/mm Hypocotyl length | 单株鲜质量/mg Fresh weight per plant | 单株干质量/mg Dry weight per plant | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | | 培养皿培养 In petri dishes | 穴盘培养 In acupoint dishes | |||
六合黄心芹 Liuhe Huangxinqin | 0 | 21.25 ab | 28.39 ab | 9.59 bc | 13.38 b | 3.82 d | 4.65 c | 0.28 c | 0.35 ab | |||
5 | 24.57 a | 31.25 a | 10.26 b | 13.41 b | 5.29 b | 5.19 b | 0.33 a | 0.36 a | ||||
10 | 23.50 a | 24.64 b | 14.59 a | 10.61 b | 5.43 a | 5.27 a | 0.33 a | 0.36 a | ||||
20 | 25.71 a | 23.87 b | 8.11 c | 13.59 b | 5.43 a | 5.20 b | 0.30 b | 0.35 ab | ||||
40 | 16.21 b | 16.31 c | 8.17 c | 26.91 a | 4.14 c | 3.32 d | 0.29 bc | 0.22 c | ||||
赛雪Saixue | 0 | 29.03 a | 28.56 abc | 14.47 a | 10.35 b | 4.20 d | 5.99 c | 0.30 c | 0.46 b | |||
5 | 31.20 a | 35.19 a | 15.13 a | 14.43 b | 4.72 b | 6.57 b | 0.36 b | 0.53 a | ||||
10 | 26.15 ab | 32.05 ab | 11.51 b | 11.07 b | 4.80 a | 6.99 a | 0.47 a | 0.42 bc | ||||
20 | 30.69 a | 21.93 c | 11.41 b | 13.89 b | 4.62 c | 5.65 d | 0.36 b | 0.37 d | ||||
40 | 16.11 b | 25.02 bc | 8.73 c | 20.01 a | 4.61 c | 6.53 b | 0.30 c | 0.38 cd |
苗龄/d Seeding age | 品种 Cultivar | NaClO/% | 株高/cm Height | 茎粗/mm Diameter | 鲜质量/mg Fresh weight | 干质量/mg Dry weight | 壮苗指数 Seedling strength index | |||
---|---|---|---|---|---|---|---|---|---|---|
地上部 Aboveground | 地下部 Underground | 地上部 Aboveground | 地下部 Underground | |||||||
40 | 六合黄心芹 Liuhe Huangxinqin | 0 | 12.10 a | 0.78 bc | 240 b | 9.50 a | 17.30 c | 0.70 b | 0.84 c | |
5 | 12.77 a | 0.98 a | 300 a | 9.10 b | 21.50 a | 0.87 a | 1.08 a | |||
10 | 10.20 b | 0.89 ab | 290 a | 7.40 c | 19.10 b | 0.73 b | 0.93 b | |||
20 | 8.80 b | 0.88 ab | 180 c | 4.10 e | 12.10 d | 0.47 c | 0.61 d | |||
40 | 6.93 c | 0.72 c | 130 d | 5.00 d | 8.00 e | 0.23 d | 0.32 e | |||
赛雪Saixue | 0 | 10.27 b | 0.96 a | 200 a | 9.90 a | 11.35 c | 0.48 b | 0.61 b | ||
5 | 12.20 a | 0.98 a | 210 a | 9.90 a | 12.50 a | 0.83 a | 0.99 a | |||
10 | 10.43 b | 0.75 b | 160 c | 4.40 b | 11.85 b | 0.27 d | 0.36 d | |||
20 | 10.37 b | 0.74 b | 150 c | 4.98 b | 9.80 d | 0.33 c | 0.41 c | |||
40 | 10.23 b | 0.75 b | 180 b | 2.78 c | 9.85 d | 0.30 cd | 0.38 cd | |||
60 | 六合黄心芹 Liuhe Huangxinqin | 0 | 13.20 b | 1.03 c | 540 b | 32.10 b | 36.20 b | 3.30 bc | 3.91 c | |
5 | 16.80 a | 1.68 a | 580 a | 89.60 a | 40.00 a | 7.70 a | 9.66 a | |||
10 | 13.40 b | 1.22 b | 370 c | 11.00 c | 23.10 c | 2.80 c | 3.37 d | |||
20 | 11.20 c | 0.95 cd | 240 d | 33.40 b | 13.90 d | 3.50 b | 4.53 b | |||
40 | 10.40 cd | 0.77 d | 180 e | 5.60 d | 8.60 e | 0.50 d | 0.60 e | |||
赛雪Saixue | 0 | 12.70 bc | 1.02 bc | 310 b | 24.80 a | 19.50 b | 2.20 a | 2.62 ab | ||
5 | 14.70 a | 1.40 a | 460 a | 25.70 a | 26.80 a | 2.20 a | 2.66 a | |||
10 | 11.90 d | 1.09 b | 290 c | 22.40 b | 17.30 b | 1.60 c | 1.92 d | |||
20 | 12.80 bc | 0.95 c | 210 d | 21.10 b | 12.90 c | 2.10 ab | 2.55 bc | |||
40 | 13.40 b | 0.86 d | 220 d | 17.50 c | 10.00 cd | 1.70 b | 2.06 cd |
Table 4 Effects of seedling indexes of 40- and 60-day-old celery seedlings under different concentrations of NaClO treatments
苗龄/d Seeding age | 品种 Cultivar | NaClO/% | 株高/cm Height | 茎粗/mm Diameter | 鲜质量/mg Fresh weight | 干质量/mg Dry weight | 壮苗指数 Seedling strength index | |||
---|---|---|---|---|---|---|---|---|---|---|
地上部 Aboveground | 地下部 Underground | 地上部 Aboveground | 地下部 Underground | |||||||
40 | 六合黄心芹 Liuhe Huangxinqin | 0 | 12.10 a | 0.78 bc | 240 b | 9.50 a | 17.30 c | 0.70 b | 0.84 c | |
5 | 12.77 a | 0.98 a | 300 a | 9.10 b | 21.50 a | 0.87 a | 1.08 a | |||
10 | 10.20 b | 0.89 ab | 290 a | 7.40 c | 19.10 b | 0.73 b | 0.93 b | |||
20 | 8.80 b | 0.88 ab | 180 c | 4.10 e | 12.10 d | 0.47 c | 0.61 d | |||
40 | 6.93 c | 0.72 c | 130 d | 5.00 d | 8.00 e | 0.23 d | 0.32 e | |||
赛雪Saixue | 0 | 10.27 b | 0.96 a | 200 a | 9.90 a | 11.35 c | 0.48 b | 0.61 b | ||
5 | 12.20 a | 0.98 a | 210 a | 9.90 a | 12.50 a | 0.83 a | 0.99 a | |||
10 | 10.43 b | 0.75 b | 160 c | 4.40 b | 11.85 b | 0.27 d | 0.36 d | |||
20 | 10.37 b | 0.74 b | 150 c | 4.98 b | 9.80 d | 0.33 c | 0.41 c | |||
40 | 10.23 b | 0.75 b | 180 b | 2.78 c | 9.85 d | 0.30 cd | 0.38 cd | |||
60 | 六合黄心芹 Liuhe Huangxinqin | 0 | 13.20 b | 1.03 c | 540 b | 32.10 b | 36.20 b | 3.30 bc | 3.91 c | |
5 | 16.80 a | 1.68 a | 580 a | 89.60 a | 40.00 a | 7.70 a | 9.66 a | |||
10 | 13.40 b | 1.22 b | 370 c | 11.00 c | 23.10 c | 2.80 c | 3.37 d | |||
20 | 11.20 c | 0.95 cd | 240 d | 33.40 b | 13.90 d | 3.50 b | 4.53 b | |||
40 | 10.40 cd | 0.77 d | 180 e | 5.60 d | 8.60 e | 0.50 d | 0.60 e | |||
赛雪Saixue | 0 | 12.70 bc | 1.02 bc | 310 b | 24.80 a | 19.50 b | 2.20 a | 2.62 ab | ||
5 | 14.70 a | 1.40 a | 460 a | 25.70 a | 26.80 a | 2.20 a | 2.66 a | |||
10 | 11.90 d | 1.09 b | 290 c | 22.40 b | 17.30 b | 1.60 c | 1.92 d | |||
20 | 12.80 bc | 0.95 c | 210 d | 21.10 b | 12.90 c | 2.10 ab | 2.55 bc | |||
40 | 13.40 b | 0.86 d | 220 d | 17.50 c | 10.00 cd | 1.70 b | 2.06 cd |
Fig. 3 Effects of chlorophyll content of celery seedlings at 20,40 and 60 d under different concentrations of NaClO treatments Different lowercase letters indicate significant difference between the two cultivars at P < 0.05 under different concentrations of NaClO treatments. The same below.
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