Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan

doi:10.16420/j.issn.0513-353x.2021-0418

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1785-1794.doi: 10.16420/j.issn.0513-353x.2021-0418

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Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan

CHEN Lilang¹, YANG Tianzhang¹, CAI Ruping¹, LIN Xiaoman¹, DENG Nankang¹, CHE Haiyan², LIN Yating², KONG Xiangyi¹^,^*()

1. Sanya Academy of Tropical Agricultural Sciences,Sanya,Hainan 572000,China
2. Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

Received:2022-04-14 Revised:2022-08-16 Online:2022-08-25 Published:2022-09-05
Contact: KONG Xiangyi E-mail:kongxiangyi20@163.com

Abstract

Abstract:

A total of 176 Passiflora edulis leaf samples showing mosaic,crinkle and deformation were collected from Sanya and Wuzhishan of Hainan Province from May to July 2020. Seven viruses were detected by PCR and RT-PCR including cucumber mosaic virus （ CMV ）,cucurbit aphid-borne yellows virus （ CABYV ）,East Asian Passiflora virus （ EAPV ）,Telosma mosaic virus （ TeMV ）,passion fruit woodiness virus （ PWV ）,Passiflora mottle virus （ PaMV ） and tomato yellow leaf curl virus （ TYLCV ）. The result showed that TeMV was the most prevalent virus with infection rate of 52.27%,followed by PaMV（43.75%）,CMV and EAPV （ 28.98% ）,PWV （ 22.73% ）,TYLCV （ 9.09% ） and CABYV （ 4.55% ）. TeMV,PaMV,CMV,EAPV and PWV may be the main viruses infecting P. edulis in Sanya and Wuzhishan. 78.91% of samples were detected co-infection with a total of 32 kinds of co-infection types,in which co-infection of two to four viruses commonly occurs. Three complete genome sequences of TYLCV from P. edulis were determined to be 2 781 bp and encoded six open reading frames （ ORFs ）. Both the identity and phylogenetic analysis based on the complete genome showed that all the three TYLCV isolates belonged to TYLCV Israel strain with nt identity ranging from 98.85% to 99.14%,and clustered in the same branch with the four TYLCV isolates from other crops in Hainan. To our knowledge,this is the first report of TYLCV in P. edulis.

Key words: Passiflora edulis, virus disease, pathogen detection, tomato yellow leaf curl virus

CLC Number:

S668.9

CHEN Lilang, YANG Tianzhang, CAI Ruping, LIN Xiaoman, DENG Nankang, CHE Haiyan, LIN Yating, KONG Xiangyi. Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan[J]. Acta Horticulturae Sinica, 2022, 49(8): 1785-1794.

Figures/Tables 6

References 30

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引物名称 Primer name	引物序列（5ʹ-3ʹ） Sequence	退火温度/℃ Annealing temperature	预期产物大小/bp Expected size	参考文献 Reference
CMV-CP fwd	TGGTCGTCCAACTATTAACCAC	53	~ 320	Kim et al.,2014
CMV-CP rev	TACTGATAAACCAGTACCGGTGA	53	~ 320	Kim et al.,2014
CABYV-F	CAAAGGACAATCTCACGGGCAGTTCC	58	~ 570	本研究This study
CABYV-R	GTTGCRTCGTGCCATTCCATCCCGTT	58
PfGSV RNA1 C13F	ATTYATGCGTTTCACGGTTA	50	~ 320	Ramos-González et al.,2020
PfGSV RNA1 C13R	CGAATGCCTCTGACAYAACT	50	~ 320	Ramos-González et al.,2020
EAPV-AO fwd	AGGATGCTAGCATGGATGAGCTAC	58	~ 900	本研究This study
EAPV-AO rev	ATGTTCTGATTGACGTCCCGTGCAG	58	~ 900	本研究This study
TeMV-CPf	TCAAGTAAGGTGGATGATGTT	49	~ 816	谢丽雪等,2017
TeMV-CPr	CTGCACAGAGCCAACCCCAA	49	~ 816	谢丽雪等,2017
PWV-F1	GGTKTGGTGCATHGAYAAYGGAAC	55	~ 400	本研究This study
PWV-R1	ACRTCVCKTGCAGTRTGCCTTTCAGT	55	~ 400	本研究This study
PaMV-F	TCACGTCTTGGACAGTTCCACAATCGC	55	~ 300	本研究This study
PaMV-R	CGACTTTAGTGACCTTGTACTGTCT	55	~ 300	本研究This study
PA	TAATATTACCKGWKGVCCSC	56	~ 580	谢艳等,2002
PB	TGGACYTTRCAWGGBCCGCACA	56	~ 580	谢艳等,2002
TYLCV-F	GTTGAGAAAACATTTGTGAATCCCC	54	~ 500	本研究This study
TYLCV-R	GGCAAAGCAACACAAAGTACGGGA	54	~ 500	本研究This study
PLDV-F	CCSATGGACTTTGGCCAGGTGTTCA	55	~ 680	本研究This study
PLDV-R	CAGGCAGAGAATGGCGTSTATATC	55	~ 680	本研究This study

引物名称 Primer name	引物序列（5ʹ-3ʹ） Sequence	退火温度/℃ Annealing temperature	预期产物大小/bp Expected size	参考文献 Reference
CMV-CP fwd	TGGTCGTCCAACTATTAACCAC	53	~ 320	Kim et al.,2014
CMV-CP rev	TACTGATAAACCAGTACCGGTGA	53	~ 320	Kim et al.,2014
CABYV-F	CAAAGGACAATCTCACGGGCAGTTCC	58	~ 570	本研究This study
CABYV-R	GTTGCRTCGTGCCATTCCATCCCGTT	58
PfGSV RNA1 C13F	ATTYATGCGTTTCACGGTTA	50	~ 320	Ramos-González et al.,2020
PfGSV RNA1 C13R	CGAATGCCTCTGACAYAACT	50	~ 320	Ramos-González et al.,2020
EAPV-AO fwd	AGGATGCTAGCATGGATGAGCTAC	58	~ 900	本研究This study
EAPV-AO rev	ATGTTCTGATTGACGTCCCGTGCAG	58	~ 900	本研究This study
TeMV-CPf	TCAAGTAAGGTGGATGATGTT	49	~ 816	谢丽雪等,2017
TeMV-CPr	CTGCACAGAGCCAACCCCAA	49	~ 816	谢丽雪等,2017
PWV-F1	GGTKTGGTGCATHGAYAAYGGAAC	55	~ 400	本研究This study
PWV-R1	ACRTCVCKTGCAGTRTGCCTTTCAGT	55	~ 400	本研究This study
PaMV-F	TCACGTCTTGGACAGTTCCACAATCGC	55	~ 300	本研究This study
PaMV-R	CGACTTTAGTGACCTTGTACTGTCT	55	~ 300	本研究This study
PA	TAATATTACCKGWKGVCCSC	56	~ 580	谢艳等,2002
PB	TGGACYTTRCAWGGBCCGCACA	56	~ 580	谢艳等,2002
TYLCV-F	GTTGAGAAAACATTTGTGAATCCCC	54	~ 500	本研究This study
TYLCV-R	GGCAAAGCAACACAAAGTACGGGA	54	~ 500	本研究This study
PLDV-F	CCSATGGACTTTGGCCAGGTGTTCA	55	~ 680	本研究This study
PLDV-R	CAGGCAGAGAATGGCGTSTATATC	55	~ 680	本研究This study

Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan

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样品来源 Sample origin	样品数 Sample number	感染病毒的样品数Number of virus-infected sample
样品来源 Sample origin	样品数 Sample number	CMV	CABYV	PfGSV	EAPV	TeMV	PWV	PaMV	TYLCV	PLDV
三亚市崖州区 Yazhou District,Sanya	10	3	0	0	0	0	5	2	2	0
三亚市吉阳区 Jiyang District,Sanya	40	6	1	0	22	36	10	37	10	0
五指山市南圣镇Nansheng Town,Wuzhishan	103	20	1	0	6	44	22	18	3	0
五指山市毛道乡Maodao Township,Wuzhishan	23	22	6	0	23	12	3	20	1	0
总数 Total	176	51	8	0	51	92	40	77	16	0

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