Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage

doi:10.16420/j.issn.0513-353x.2021-0597

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1582-1588.doi: 10.16420/j.issn.0513-353x.2021-0597

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Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage

ZHANG Lugang(), LU Qianqian, HE Qiong, XUE Yihua, MA Xiaomin, MA Shuai, NIE Shanshan, YANG Wenjing

State Key Laboratory of Crop Stress Biology in Arid Regions,College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China

Received:2022-02-28 Revised:2022-03-24 Online:2022-07-25 Published:2022-07-29
Contact: ZHANG Lugang E-mail:lugangzh@163.com

Abstract

Abstract:

In order to enrich the germplasm resources of Chinese cabbage and improve the functional composition,and create a new purple-orange heading Chinese cabbage germplasm riched in anthocyanins and carotenoids simultaneously. A purple heading Chinese cabbage pure line 14S838 and an orange heading Chinese cabbage pure line 14S125 were hybridized,and their F₂ was produced by single F₁ plant selfing. Then the excellent heading plants carrying purple heading gene and orange heading gene in the F₂ population were selected by molecular markers combining field horticultural traits observation,lately,the selected plants was self-crossed and selected for three generations continuously. Finally,four new Chinese cabbage germplasm with the green outer leaves and purple-orange heading leaves,such as 19ZS40-1,19ZS17-1,19ZS94 and 19SF19-4,were bred. This study first successfully aggregated the purple heading gene and orange heading gene in one plant of Chinese cabbage together,promote these two gene normal expression,and created the novel germplasms of Chinese cabbage simultaneously accumulated both carotenoids and anthocyanins,which has greatly improved the nutritional value,enrich the color heading Chinese cabbage breeding materials,laid a new foundation for the nutritional quality breeding of Chinese cabbage.

Key words: Chinese cabbage, leaf-head color, carotenoids, anthocyanins, hybridization, molecular marker

CLC Number:

S634.1

ZHANG Lugang, LU Qianqian, HE Qiong, XUE Yihua, MA Xiaomin, MA Shuai, NIE Shanshan, YANG Wenjing. Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage[J]. Acta Horticulturae Sinica, 2022, 49(7): 1582-1588.

Figures/Tables 6

Fig. 1 Performance of cross section of parents 14S125（♀,orange）and 14S838（♂,purple）and F2 plants leaf head of Chinese cabbage（b1-b6）

Fig. 2 Identification of orange gene in pure orange line（1-18）of purple-orange Chinese cabbage with molecular marker

Fig. 3 Identification of purple gene in purple-orange Chinese cabbage with molecular marker 4,6,7,10,12,13,14,18 are homozygous lines at purple gene.

Fig. 4 Phenotype of leaf head and longitudinal section of purple-orange Chinese cabbage

Table 1 Characteristics of four purple-orange Chinese cabbages

编号 Code	株幅/cm Plant width	株高/cm Plant height	外叶数 Number of outer leaves	球高/cm Head height	球径/cm Head width	毛菜质量/kg Gross weight	净菜质量/kg Net weight
19ZS40-1	48	30	7	25	18	1.21	0.81
19ZS17-1	60	30	10	28	16	1.76	0.66
19ZS94	57	33	8	22	15	1.02	0.51
19SF19-4	51	27	6	22	14	1.11	0.80

Fig. 5 Content of anthocyanin and carotenoids in differant inbred line of purple-orange Chinese cabbage

References 16

[1]	Duan Yanjiao, Zhang Lugang, He Qiong, Zhang Mingke, Shi Jiangchao. 2012. Expression of transcriptional factors and structural genes of anthocyanin biosynthesis in purple-heading Chinese cabbage. Acta Horticulturae Sinica, 39 (11):2159-2167. (in Chinese)
	段岩娇, 张鲁刚, 何琼, 张明科, 石姜超. 2012. 紫心大白菜花青素积累特性及相关基因表达分析. 园艺学报, 39 (11):2159-2167.
[2]	He Q, Zhang Z F, Zhang L G. 2016. Anthocyanin accumulation,antioxidant ability and stability,and a transcriptional analysis of anthocyanin biosynthesis in purple heading Chinese cabbage(Brassica rapa L. ssp. pekinensis). Journal of Agricultural and Food Chemistry, 64 (1):132-145. doi: 10.1021/acs.jafc.5b04674 URL
[3]	He Q, Wu J Q, Xue Y H, Zhao W B, Li R, Zhang L G. 2020. The novel gene BrMYB2,located on chromosome A07,with a short intron 1 controls the purple-head trait of Chinese cabbage(Brassica rapa L.). Horticulture Research, 7:97. doi: 10.1038/s41438-020-0319-z URL
[4]	Mo Yunan, Zhang Lugang, Wang Guofang. 2014. Extraction and determination of total carotenoids in orange heading Chinese cabbage. Journal of Northwest A & F University(Natural Science Edition), 42 (3):206-214. (in Chinese)
	莫玉楠, 张鲁刚, 王国芳. 2014. 橙色大白菜类胡萝卜素提取与测定方法研究. 西北农林科技大学学报(自然科学版), 42 (3):206-214.
[5]	Sun Rifei, Zhang Shujiang, Zhang Shifan, Li Fei. 2006. Research on creation of purple Chinese cabbage germplasm. Acta Horticulturae Sinica, 33 (5):1032. (in Chinese)
	孙日飞, 张淑江, 章时蕃, 李菲. 2006. 紫红色大白菜种质的创新研究. 园艺学报, 33 (5):1032.
[6]	Wu Junqing. 2016. Genetic analysis and fine mapping of the purple gene in purple heading Chinses cabbage[M. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	吴俊清. 2016. 紫心大白菜遗传规律分析及紫色基因精细定位[硕士论文]. 杨凌: 西北农林科技大学
[7]	Xue Yihua, He Qiong, Chen Lin, Li Ru, Zhang Lugang. 2019. Genetic analysis and measurement parameter comparison of the purple trait in purple heading Chinese cabbage. Acta Horticulturae Sinica, 46 (12):2423-2428. (in Chinese)
	薛一花, 何琼, 陈霖, 李茹, 张鲁刚. 2019. 大白菜紫心性状的遗传分析及度量参数比较. 园艺学报, 46 (12):2423-2428.
[8]	Xu Jiabing. 1998. Beijing orange heart(97-8)new line of Chinese cabbage. Chinese Vegetables, 1 (4):31. (in Chinese)
	徐家炳. 1998. 北京桔红心(97-8)大白菜新品系. 中国蔬菜, 1 (4):31.
[9]	Zhang Deshuang, Zhang Fenglan, Yu Yangjun, Zhao Xiuyun, Yu Shuancang, Xu Jiaping. 2007. The creation of purple Chinese cabbage breeding materials. Journal of Changjiang Vegetables,(11):52-53. (in Chinese)
	张德双, 张凤兰, 余阳俊, 赵岫云, 于栓仓, 徐家炳. 2007. 紫色大白菜育种材料的创造. 长江蔬菜,(11):52-53.
[10]	Zhang Deshuang, Zhao Hong, Zhang Fenglan, Yu Yangjun, Zhao Xiuyun, Yu Shuancang, Wang Weihong, Su Tongbing, Lu Guixiang. 2017. Identification and RNA-seq of new purple Chinese cabbage 15NG28 progenies. Acta Agriculturae Boreali-Sinica, 32 (6):14-24. (in Chinese)
	张德双, 赵泓, 张凤兰, 余阳俊, 赵岫云, 于拴仓, 汪维红, 苏同兵, 卢桂香. 2017. 新型紫色大白菜15NG28后代的鉴别及其转录组分析. 华北农学报, 32 (6):14-24.
[11]	Zhang Deshuang, Zhang Fenglan, Yu Yangjun, Zhao Xiuyun, Yu Shuancang, Wang Weihong, Su Tongbing, Lu Guixiang. 2018. Progress on heading colors of Chinese cabbage. Vegetables,(2):30-35. (in Chinese)
	张德双, 张凤兰, 余阳俊, 赵岫云, 于拴仓, 汪维红, 苏同兵, 卢桂香. 2018. 白菜球色的研究进展. 蔬菜,(2):30-35.
[12]	Zhang J X, Li H X, Zhang M K, Hui M X, Wang Q, Li L, Zhang L G. 2013. Fine mapping and identification of candidate Br-or gene controlling orange head of Chinese cabbage(Brassica rapa L. ssp. pekinensis). Molecular Breeding, 32 (4):799-805. doi: 10.1007/s11032-013-9907-z URL
[13]	Zhang J X, Yuan H, Fei Z J, Pogson Barry J, Zhang L G, Li L. 2015. Molecular characterization and transcriptome analysis of orange head Chinese cabbage(Brassica rapa). Planta, 241:1381-1394. doi: 10.1007/s00425-015-2262-z URL
[14]	Zhang Lugang, Hui Maixia, Zhang Mingke. 2005. Breeding of color Chinese cabbage-“Jinguan No. 1”. Northern Horticulture,(4):67-68,97. (in Chinese)
	张鲁刚, 惠麦侠, 张明科. 2005. 彩色大白菜新品种“金冠1号”的选育. 北方园艺,(4):67-68,97.
[15]	Zhang Lugang, Zhang Mingke, Hui Maixia. 2009. A method for new germplasm breeding of purple-heading Chinese cabbage(Patent number:ZL200910021165.6;Application time:2009.02.18;Authorization and publish time:2011.3.23. (in Chinese)
	张鲁刚, 张明科, 惠麦侠. 2009. 一种紫心大白菜新种质的选育方法(专利号:ZL200910021165.6;申请时间 2009.02.18;授权公告日2011.3.23).
[16]	Zhou Zhenqing. 1992. Hybrid of turnips and Chinese cabbage-orange queen. Chinese Vegetables,(2):55. (in Chinese)
	周振清. 1992. 芜菁与白菜杂交种--橙色女王. 中国蔬菜,(2):55.

Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage

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