Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

doi:10.16420/j.issn.0513-353x.2021-0499

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1401-1414.doi: 10.16420/j.issn.0513-353x.2021-0499

• Research Papers • Next Articles

Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

ZHENG Xiaodong¹, XI Xiangli¹, LI Yuqi¹, SUN Zhijuan², MA Changqing¹, HAN Mingsan³, LI Shaoxuan³, TIAN Yike¹, WANG Caihong¹^,^**()

¹Laboratory of Fruit Tree Genetic Improvement and Biotechnology,College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China
²College of Life Science,Qingdao Agricultural University,Qingdao, Shandong 266109,China
³Qingdao Academy of Agricultural Sciences,Qingdao,Shandong 266000,China

Received:2022-01-17 Revised:2022-03-29 Online:2022-07-25 Published:2022-07-29
Contact: WANG Caihong E-mail:chw6068@126.com

Abstract

Abstract:

In order to explore the effect of brassinosteroids on the growth of apple plants under saline-alkali stress,apple rootstock Malus hupehensis with apomixis characteristics were used as experimental materials. The study investigated the effects of exogenous EBL（brassinosteroids analogs）on ion homeostasis,osmotic regulation,antioxidant system and pH balance of M. hupehensis under saline-alkali stress. The results showed that application of 0.2 mg · L^-1 exogenous EBL could significantly enhance the tolerance of M. hupehensis to saline-alkali stress. The main mechanisms were as follows:exogenous EBL could regulate the expression of Na⁺/K⁺transporter genes and enhance the ratio of potassium to sodium to maintain ion homeostasis. Moreover,exogenous EBL could accumulate proline and soluble sugar to modulate osmotic stress,and enhance the activities of POD and CAT to alleviate oxidative damage under saline-alkali stress. Furthermore,exogenous EBL could decrease root pH by improving root secretion of organic acids and enhancing the expression of MhAHA2 and MhAHA8 genes. Exogenous EBL could also cooperate with auxin,gibberellin and dihydrozeatin responding to saline-alkali stress.

Key words: apple, Malus hupehensis, rootstock, saline-alkali stress, brassinosteroids, ion homeostasis, oxidative damage

CLC Number:

S661.1

ZHENG Xiaodong, XI Xiangli, LI Yuqi, SUN Zhijuan, MA Changqing, HAN Mingsan, LI Shaoxuan, TIAN Yike, WANG Caihong. Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress[J]. Acta Horticulturae Sinica, 2022, 49(7): 1401-1414.

Figures/Tables 12

Table 1 Primer sequences for qRT-PCR

基因名称 Gene name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
MhCAX5	ACCAGTCTCACTCTTTGTGGCG	GAATATAATTGGTGGGAGGAGATAT
MhCHX15	CCTCTTGGTACAGCATTGATAAAAA	GTTTGAACTTAATTTTGCAGCACA
MhSOS1	TACATCATTTCTGGTATATCTTGTG	CAAGATGAAAATTAAGGTATTAGCA
MhALT1	GTTGTTTTTTTGCTCTGACGACT	GTTGAACCACAAAACCCTGCT
MhSKOR	CATCCTGACAACTGGTGGTATCG	AAGTACCTCAGAGCAATCCGTTT
MhNHX4	ACGAAACTCCTTTACTATACAGCCT	TGATACCACAGATAAGTGAGCATAG
MhAHA2	AAAGTGTGAAGAAAGAGAAAATGC	AGTTCATTTGCTTGACATTCTTT
MhAHA8	GGTCAAAAGTGTGTTGGTAAGCA	AAACCAAAATCCTCCAACAGC
MhMAPKKKa	GCTACATGGGACATGACATTACTAG	CCAAGATGTTCCTGCCTTTTAT
MhLECPK91	TTCACCACTTATGCCAATGATAC	GGAACTGGAGATTCTTGCTTTTT
MhERF109	ATGCCCTTCCATGCGAATAG	CTAGTTCATGGACAACCATGCC
MhNAC56	ATGGAGTGCACCGACTCGTC	CTATCCCAAATTGGACTCAGAATAC
MhActin	CTTCAATGTGCCTGCCATGTAT	AATTTCCCGTTCAGCAGTAGTG

Fig. 1 The phenotype of Malus hupehensis under saline-alkali stress and exogenous EBL treatment at 0 and 15 d

Fig. 2 Effects of saline-alkali stress and exogenous EBL treatment on wilting rate,fresh weight and dry weight of Malus hupehensis seedlingsDifferent lowercase letters indicate significant difference at the 0.05 probability level.

Fig. 3 Effects of saline-alkali stress and exogenous EBL treatment on chlorophyll content and photosynthetic rate of Malus hupehensis

Fig. 4 Effects of saline-alkali stress and exogenous EBL treatment on ion content of Malus hupehensis

Fig. 5 Effects of saline-alkali stress and exogenous EBL treatment on the content of proline,soluble sugar,and soluble protein in Malus hupehensis

Fig. 6 Effects of saline-alkali stress and exogenous EBL treatment on the content of MDA,superoxide anion,H2O2 and the activities of autioxidant enzyme in Malus hupehensis

Fig. 7 Effects of saline-alkali stress and exogenous EBL treatment on root of Malus hupehensis by dyeing of TTC

Fig. 8 Effects of saline-alkali stress and exogenous EBL treatment on malic acid and citric acid content in Malus hupehensis leaves

Fig. 9 Effects of saline-alkali stress and exogenous EBL treatment on the contents of endogenous hormones in Malus hupehensis leaves

Fig. 10 Effects of saline-alkali stress and exogenous EBL treatment on the expression of saline-alkali stress response genes in Malus hupehensis

Fig. 11 Pattern diagram of exogenous EBL enhancing salt-alkali tolerance of Malus hupehensis

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Effects and Regulating Mechanism of Exogenous Brassinosteroids on the Growth of Malus hupehensis Under Saline-alkali Stress

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