Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1351-1362.doi: 10.16420/j.issn.0513-353x.2021-0767
• Research Notes • Previous Articles Next Articles
QIU Keli1,2, WANG Yumin3, HE Jinling2, YU Hong1,4, PAN Haifa1, SHENG Yu1, XIE Qingmei1, CHEN Hongli1, ZHOU Hui1,**(), ZHANG Jinyun1,**(
)
Received:
2021-10-29
Revised:
2021-12-14
Online:
2022-06-25
Published:
2022-07-05
Contact:
ZHOU Hui,ZHANG Jinyun
E-mail:huichou1987@126.com;zjy600@aaas.org.cn
CLC Number:
QIU Keli, WANG Yumin, HE Jinling, YU Hong, PAN Haifa, SHENG Yu, XIE Qingmei, CHEN Hongli, ZHOU Hui, ZHANG Jinyun. Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21[J]. Acta Horticulturae Sinica, 2022, 49(6): 1351-1362.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2021-0767
基因 Gene | 引物序列(5′-3′) Primer sequence |
---|---|
TEF2 | F:GGTGTGACGATGAAGAGTGATG:R:TGAAGGAGAGGGAAGGTGAAAG |
PpLAC7 | F:AGGGCAGATAACCCAGGCA:R:AGGTGGTCGCAGAGTCTCATT |
PpLAC15 | F:CCTAAAACTATCGACAAGCGAGTT:R:TGACAAGGAGGGACGGACAA |
PpLAC19 | F:GGGCTGATAATCCAGGAGTTTG:R:CCTTTGCCATTGTCCACCA |
PpLAC20 | F:GGGTGGCTATCCGATTTCTG:R:AGCTTCCCATCCTGGACTACC |
PpLAC21 | F:CGACCCTGTTGAAAGAAATACC:R:CCATACACCGGGATTATCTGCT |
PpLAC22 | F:GCCCAAATGTCTCTGATGCTTA:R:CTCATCATTCAGTGCTGCGTT |
PpLAC24 | F:CACCAAACTCCTCGGATGC:R:TCCCTTGCTCAGCCTCCAT |
PpLAC27 | F:GCTGGCGGTTGGATTGC:R:GATCCACGCCATCCTTAGC |
PpLAC28 | F:CCACCCACTTCACCTCCAT:R:CGCCAACGGTGTTCCTCT |
PpLAC30 | F:GCTATCCGATTTCAAGCAGATAA:R:TTGATTCATTCGGTCCTTTCC |
PpLAC33 | F:TGGCACCAAACACCTCTGAC:R:CCCTTGCTCAACCTCCATAACA |
PpLAC43 | F:GCACTACTCACTGCCAACCTT:R:TGAAGTTCCCAGTTCCATCCA |
PpLAC44 | F:TCTCATCCCTTCCACCTTCAT:R:ATACTTTGCCGGGTCCTGTT |
PpLAC45 | F:GCTGCAAGACACCAATCTTCTC:R:GGCAACCCAACCACCTGTA |
Table 1 Primer sequence used for qRT-PCR
基因 Gene | 引物序列(5′-3′) Primer sequence |
---|---|
TEF2 | F:GGTGTGACGATGAAGAGTGATG:R:TGAAGGAGAGGGAAGGTGAAAG |
PpLAC7 | F:AGGGCAGATAACCCAGGCA:R:AGGTGGTCGCAGAGTCTCATT |
PpLAC15 | F:CCTAAAACTATCGACAAGCGAGTT:R:TGACAAGGAGGGACGGACAA |
PpLAC19 | F:GGGCTGATAATCCAGGAGTTTG:R:CCTTTGCCATTGTCCACCA |
PpLAC20 | F:GGGTGGCTATCCGATTTCTG:R:AGCTTCCCATCCTGGACTACC |
PpLAC21 | F:CGACCCTGTTGAAAGAAATACC:R:CCATACACCGGGATTATCTGCT |
PpLAC22 | F:GCCCAAATGTCTCTGATGCTTA:R:CTCATCATTCAGTGCTGCGTT |
PpLAC24 | F:CACCAAACTCCTCGGATGC:R:TCCCTTGCTCAGCCTCCAT |
PpLAC27 | F:GCTGGCGGTTGGATTGC:R:GATCCACGCCATCCTTAGC |
PpLAC28 | F:CCACCCACTTCACCTCCAT:R:CGCCAACGGTGTTCCTCT |
PpLAC30 | F:GCTATCCGATTTCAAGCAGATAA:R:TTGATTCATTCGGTCCTTTCC |
PpLAC33 | F:TGGCACCAAACACCTCTGAC:R:CCCTTGCTCAACCTCCATAACA |
PpLAC43 | F:GCACTACTCACTGCCAACCTT:R:TGAAGTTCCCAGTTCCATCCA |
PpLAC44 | F:TCTCATCCCTTCCACCTTCAT:R:ATACTTTGCCGGGTCCTGTT |
PpLAC45 | F:GCTGCAAGACACCAATCTTCTC:R:GGCAACCCAACCACCTGTA |
基因 Gene | ID | 蛋白质长度/aa Protein length | 等电点 pI | 分子量/D Relative molecular mass | 亚细胞定位 Subcellular localization |
---|---|---|---|---|---|
PpLAC1 | Prupe.1G323200 | 578 | 9.18 | 65 744.34 | 过氧化物酶体 Peroxisome |
PpLAC2 | Prupe.1G357100 | 544 | 9.18 | 60 477.29 | 细胞外基质 Extracellular matrix |
PpLAC3 | Prupe.1G559300 | 568 | 8.98 | 64 123.39 | 溶酶体 Lysosome |
PpLAC4 | Prupe.2G191900 | 574 | 8.38 | 63 604.68 | 细胞外基质 Extracellular matrix |
PpLAC5 | Prupe.2G205500 | 584 | 5.40 | 64 495.92 | 溶酶体 Lysosome |
PpLAC6 | Prupe.2G205600 | 609 | 6.74 | 67 760.86 | 溶酶体 Lysosome |
PpLAC7 | Prupe.2G245800 | 558 | 9.02 | 61 240.19 | 细胞外基质 Extracellular matrix |
PpLAC8 | Prupe.2G257400 | 583 | 8.53 | 64 070.85 | 细胞外基质 Extracellular matrix |
PpLAC9 | Prupe.2G278000 | 592 | 6.54 | 66 358.74 | 细胞外基质 Extracellular matrix |
PpLAC10 | Prupe.2G325200 | 567 | 6.82 | 62 943.79 | 溶酶体 Lysosome |
PpLAC11 | Prupe.3G216000 | 542 | 8.39 | 60 174.81 | 细胞外基质 Extracellular matrix |
PpLAC12 | Prupe.4G018800 | 543 | 9.72 | 61 649.96 | 细胞外基质 Extracellular matrix |
PpLAC13 | Prupe.4G131900 | 597 | 7.99 | 67 085.54 | 细胞外基质 Extracellular matrix |
PpLAC14 | Prupe.5G075700 | 591 | 8.40 | 65 813.66 | 质膜 Plasma membrane |
PpLAC15 | Prupe.5G231100 | 582 | 7.06 | 65 497.57 | 溶酶体 Lysosome |
PpLAC16 | Prupe.6G000400 | 552 | 9.01 | 61 928.85 | 细胞外基质 Extracellular matrix |
PpLAC17 | Prupe.6G000500 | 554 | 8.84 | 62 137.61 | 细胞外基质 Extracellular matrix |
PpLAC18 | Prupe.6G048400 | 1 559 | 6.47 | 175 147.62 | 质膜 Plasma membrane |
PpLAC19 | Prupe.6G061300 | 558 | 9.43 | 61 628.12 | 细胞外基质 Extracellular matrix |
PpLAC20 | Prupe.6G072100 | 587 | 9.77 | 64 558.17 | 细胞外基质 Extracellular matrix |
PpLAC21 | Prupe.6G089100 | 583 | 9.33 | 64 487.37 | 溶酶体 Lysosome |
PpLAC22 | Prupe.6G089300 | 581 | 9.30 | 64 283.15 | 细胞外基质 Extracellular matrix |
PpLAC23 | Prupe.6G155100 | 577 | 6.81 | 64 268.78 | 细胞外基质 Extracellular matrix |
PpLAC24 | Prupe.6G177700 | 564 | 8.41 | 62 267.07 | 溶酶体 Lysosome |
PpLAC25 | Prupe.6G179500 | 580 | 7.09 | 65 306.02 | 细胞外基质 Extracellular matrix |
基因 Gene | ID | 蛋白质长度/aa Protein length | 等电点 pI | 分子量/D Relative molecular mass | 亚细胞定位 Subcellular localization |
PpLAC26 | Prupe.6G242000 | 567 | 6.64 | 63 053.79 | 溶酶体 Lysosome |
PpLAC27 | Prupe.6G257700 | 585 | 9.02 | 64 453.73 | 质膜 Plasma membrane |
PpLAC28 | Prupe.6G258000 | 586 | 9.85 | 64 986.03 | 细胞外基质 Extracellular matrix |
PpLAC29 | Prupe.6G267700 | 563 | 5.95 | 61 867.26 | 细胞外基质 Extracellular matrix |
PpLAC30 | Prupe.6G271500 | 559 | 9.31 | 60 774.70 | 细胞外基质 Extracellular matrix |
PpLAC31 | Prupe.7G140500 | 539 | 9.29 | 60 306.38 | 细胞外基质 Extracellular matrix |
PpLAC32 | Prupe.7G140600 | 537 | 9.46 | 59 617.38 | 溶酶体 Lysosome |
PpLAC33 | Prupe.7G156500 | 587 | 8.65 | 65 072.51 | 细胞外基质 Extracellular matrix |
PpLAC34 | Prupe.8G046800 | 568 | 6.76 | 64 097.74 | 细胞外基质 Extracellular matrix |
PpLAC35 | Prupe.8G046900 | 578 | 9.08 | 65 169.63 | 细胞外基质 Extracellular matrix |
PpLAC36 | Prupe.8G047000 | 584 | 4.95 | 65 244.50 | 细胞外基质 Extracellular matrix |
PpLAC37 | Prupe.8G047100 | 570 | 5.52 | 62 952.86 | 质膜 Plasma membrane |
PpLAC38 | Prupe.8G047200 | 569 | 5.29 | 62 540.13 | 质膜 Plasma membrane |
PpLAC39 | Prupe.8G047300 | 569 | 5.11 | 62 344.97 | 质膜 Plasma membrane |
PpLAC40 | Prupe.8G047500 | 558 | 5.52 | 61 255.84 | 质膜 Plasma membrane |
PpLAC41 | Prupe.8G047900 | 564 | 5.58 | 62 057.08 | 质膜 Plasma membrane |
PpLAC42 | Prupe.8G048100 | 564 | 5.93 | 62 274.45 | 细胞外基质 Extracellular matrix |
PpLAC43 | Prupe.8G094800 | 367 | 9.69 | 40 256.36 | 质膜 Plasma membrane |
PpLAC44 | Prupe.8G095000 | 514 | 8.63 | 57 389.05 | 溶酶体 Lysosome |
PpLAC45 | Prupe.8G095400 | 563 | 8.78 | 62 198.39 | 溶酶体 Lysosome |
PpLAC46 | Prupe.8G097000 | 563 | 8.78 | 62 158.28 | 溶酶体 Lysosome |
PpLAC47 | Prupe.8G172900 | 536 | 9.26 | 59 889.78 | 细胞外基质 Extracellular matrix |
PpLAC48 | Prupe.8G189700 | 588 | 5.94 | 65 150.69 | 溶酶体 Lysosome |
Table 2 Physicochemical properties and subcellular localization prediction of peach laccase protein
基因 Gene | ID | 蛋白质长度/aa Protein length | 等电点 pI | 分子量/D Relative molecular mass | 亚细胞定位 Subcellular localization |
---|---|---|---|---|---|
PpLAC1 | Prupe.1G323200 | 578 | 9.18 | 65 744.34 | 过氧化物酶体 Peroxisome |
PpLAC2 | Prupe.1G357100 | 544 | 9.18 | 60 477.29 | 细胞外基质 Extracellular matrix |
PpLAC3 | Prupe.1G559300 | 568 | 8.98 | 64 123.39 | 溶酶体 Lysosome |
PpLAC4 | Prupe.2G191900 | 574 | 8.38 | 63 604.68 | 细胞外基质 Extracellular matrix |
PpLAC5 | Prupe.2G205500 | 584 | 5.40 | 64 495.92 | 溶酶体 Lysosome |
PpLAC6 | Prupe.2G205600 | 609 | 6.74 | 67 760.86 | 溶酶体 Lysosome |
PpLAC7 | Prupe.2G245800 | 558 | 9.02 | 61 240.19 | 细胞外基质 Extracellular matrix |
PpLAC8 | Prupe.2G257400 | 583 | 8.53 | 64 070.85 | 细胞外基质 Extracellular matrix |
PpLAC9 | Prupe.2G278000 | 592 | 6.54 | 66 358.74 | 细胞外基质 Extracellular matrix |
PpLAC10 | Prupe.2G325200 | 567 | 6.82 | 62 943.79 | 溶酶体 Lysosome |
PpLAC11 | Prupe.3G216000 | 542 | 8.39 | 60 174.81 | 细胞外基质 Extracellular matrix |
PpLAC12 | Prupe.4G018800 | 543 | 9.72 | 61 649.96 | 细胞外基质 Extracellular matrix |
PpLAC13 | Prupe.4G131900 | 597 | 7.99 | 67 085.54 | 细胞外基质 Extracellular matrix |
PpLAC14 | Prupe.5G075700 | 591 | 8.40 | 65 813.66 | 质膜 Plasma membrane |
PpLAC15 | Prupe.5G231100 | 582 | 7.06 | 65 497.57 | 溶酶体 Lysosome |
PpLAC16 | Prupe.6G000400 | 552 | 9.01 | 61 928.85 | 细胞外基质 Extracellular matrix |
PpLAC17 | Prupe.6G000500 | 554 | 8.84 | 62 137.61 | 细胞外基质 Extracellular matrix |
PpLAC18 | Prupe.6G048400 | 1 559 | 6.47 | 175 147.62 | 质膜 Plasma membrane |
PpLAC19 | Prupe.6G061300 | 558 | 9.43 | 61 628.12 | 细胞外基质 Extracellular matrix |
PpLAC20 | Prupe.6G072100 | 587 | 9.77 | 64 558.17 | 细胞外基质 Extracellular matrix |
PpLAC21 | Prupe.6G089100 | 583 | 9.33 | 64 487.37 | 溶酶体 Lysosome |
PpLAC22 | Prupe.6G089300 | 581 | 9.30 | 64 283.15 | 细胞外基质 Extracellular matrix |
PpLAC23 | Prupe.6G155100 | 577 | 6.81 | 64 268.78 | 细胞外基质 Extracellular matrix |
PpLAC24 | Prupe.6G177700 | 564 | 8.41 | 62 267.07 | 溶酶体 Lysosome |
PpLAC25 | Prupe.6G179500 | 580 | 7.09 | 65 306.02 | 细胞外基质 Extracellular matrix |
基因 Gene | ID | 蛋白质长度/aa Protein length | 等电点 pI | 分子量/D Relative molecular mass | 亚细胞定位 Subcellular localization |
PpLAC26 | Prupe.6G242000 | 567 | 6.64 | 63 053.79 | 溶酶体 Lysosome |
PpLAC27 | Prupe.6G257700 | 585 | 9.02 | 64 453.73 | 质膜 Plasma membrane |
PpLAC28 | Prupe.6G258000 | 586 | 9.85 | 64 986.03 | 细胞外基质 Extracellular matrix |
PpLAC29 | Prupe.6G267700 | 563 | 5.95 | 61 867.26 | 细胞外基质 Extracellular matrix |
PpLAC30 | Prupe.6G271500 | 559 | 9.31 | 60 774.70 | 细胞外基质 Extracellular matrix |
PpLAC31 | Prupe.7G140500 | 539 | 9.29 | 60 306.38 | 细胞外基质 Extracellular matrix |
PpLAC32 | Prupe.7G140600 | 537 | 9.46 | 59 617.38 | 溶酶体 Lysosome |
PpLAC33 | Prupe.7G156500 | 587 | 8.65 | 65 072.51 | 细胞外基质 Extracellular matrix |
PpLAC34 | Prupe.8G046800 | 568 | 6.76 | 64 097.74 | 细胞外基质 Extracellular matrix |
PpLAC35 | Prupe.8G046900 | 578 | 9.08 | 65 169.63 | 细胞外基质 Extracellular matrix |
PpLAC36 | Prupe.8G047000 | 584 | 4.95 | 65 244.50 | 细胞外基质 Extracellular matrix |
PpLAC37 | Prupe.8G047100 | 570 | 5.52 | 62 952.86 | 质膜 Plasma membrane |
PpLAC38 | Prupe.8G047200 | 569 | 5.29 | 62 540.13 | 质膜 Plasma membrane |
PpLAC39 | Prupe.8G047300 | 569 | 5.11 | 62 344.97 | 质膜 Plasma membrane |
PpLAC40 | Prupe.8G047500 | 558 | 5.52 | 61 255.84 | 质膜 Plasma membrane |
PpLAC41 | Prupe.8G047900 | 564 | 5.58 | 62 057.08 | 质膜 Plasma membrane |
PpLAC42 | Prupe.8G048100 | 564 | 5.93 | 62 274.45 | 细胞外基质 Extracellular matrix |
PpLAC43 | Prupe.8G094800 | 367 | 9.69 | 40 256.36 | 质膜 Plasma membrane |
PpLAC44 | Prupe.8G095000 | 514 | 8.63 | 57 389.05 | 溶酶体 Lysosome |
PpLAC45 | Prupe.8G095400 | 563 | 8.78 | 62 198.39 | 溶酶体 Lysosome |
PpLAC46 | Prupe.8G097000 | 563 | 8.78 | 62 158.28 | 溶酶体 Lysosome |
PpLAC47 | Prupe.8G172900 | 536 | 9.26 | 59 889.78 | 细胞外基质 Extracellular matrix |
PpLAC48 | Prupe.8G189700 | 588 | 5.94 | 65 150.69 | 溶酶体 Lysosome |
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