Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume

doi:10.16420/j.issn.0513-353x.2021-0468

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1327-1338.doi: 10.16420/j.issn.0513-353x.2021-0468

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Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume

LIU Yaoyao, WU Yanyan, Shi Yan, MAO Tianyu, BAO Manzhu, ZHANG Junwei, ZHANG Jie()

Key Laboratory of Horticultural Plant Biology of Ministry of Education,College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan 430070,China

Received:2021-12-27 Revised:2022-03-30 Online:2022-06-25 Published:2022-07-05
Contact: ZHANG Jie E-mail:flybebrave@mail.hzau.edu.cn

Abstract

Abstract:

A candidate gene PmTAC1（TILLER ANGLE CONTROL 1）of the weeping trait of Prunus mume was cloned and analyzed based on our previous transcriptome data. The coding sequence of PmTAC1 was 915 bp,encoding 304 amino acids,and no differences was observed between upright and weeping cultivars of mei. The amino acid sequence alignment further confirmed that PmTAC1 had the typical domain of the IGT family,and evolutionary analysis showed that the genetic relationship between PmTAC1 and PpeTAC1 was the closest. Prediction of subcellular localization and hydrophobic analysis of protein showed that the protein was located in the nucleus and chloroplast,and was hydrophilic. The expression of PmTAC1 in branches of weeping cultivars was significantly higher than that in 1-year-old branches of upright cultivars,and there were differences in the adaxial and abaxial of the two types of branches. The promoter sequences of PmTAC1 in weeping and upright mei were 1 608 bp and 1 379 bp respectively,which were consistent among different cultivars of the same branch types of mei. Differences were detected in the number of light responses,gibberellin,and abscisic acid response cis-elements between the two promoter sequences. In conclusion,the promoter sequence and expression level of PmTAC1 in weeping and upright cultivars of mei may be related to the formation of weeping traits.

Key words: Prunus mume, weeping trait, PmTAC1, gene cloning, expression

CLC Number:

S685.17

LIU Yaoyao, WU Yanyan, Shi Yan, MAO Tianyu, BAO Manzhu, ZHANG Junwei, ZHANG Jie. Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume[J]. Acta Horticulturae Sinica, 2022, 49(6): 1327-1338.

Figures/Tables 11

References 37

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用途 Primer use	引物名称 Primer name	引物序列（5′-3′） Primer sequnce
克隆Cloning	PmTAC1-F	ATGAAGATCTTCAACTGGGTTC
	PmTAC1-R	TCAGTGCACACAAGGGGCACCTTG
启动子克隆Promoter cloning	PmTAC1-Pro-F	TGAAGCCTCTTATGAAC
	PmTAC1-Pro-R	CAGAAAGTAACCCACCT
实时荧光定量qRT-PCR	PmTAC1-qRTF	AAACCAATGACAAGGACACAC
	PmTAC1-qRTR	CGCTCTCCAGAACGAAAT
	ACTIN2- qRTF	TGTGATGGTTGGTATGGGG
	ACTIN2- qRTR	AACTGGATGCTCTTCTGGG

用途 Primer use	引物名称 Primer name	引物序列（5′-3′） Primer sequnce
克隆Cloning	PmTAC1-F	ATGAAGATCTTCAACTGGGTTC
	PmTAC1-R	TCAGTGCACACAAGGGGCACCTTG
启动子克隆Promoter cloning	PmTAC1-Pro-F	TGAAGCCTCTTATGAAC
	PmTAC1-Pro-R	CAGAAAGTAACCCACCT
实时荧光定量qRT-PCR	PmTAC1-qRTF	AAACCAATGACAAGGACACAC
	PmTAC1-qRTR	CGCTCTCCAGAACGAAAT
	ACTIN2- qRTF	TGTGATGGTTGGTATGGGG
	ACTIN2- qRTR	AACTGGATGCTCTTCTGGG

cis-Element	垂枝Weeping	直枝 Upright	功能 Function
GATA-motif	1	1	光响应Part of a light responsive element
I-box	2	2	光响应Part of a light responsive element
AAAC-motif	1	0	光响应Light responsive element
GT1-motif	2	0	光响应Light responsive element
G-box	0	1	光响应cis-Acting regulatory element involved in light responsiveness
TCCC-motif	0	1	光响应Part of a light responsive element
CAAT-box	29	24	增强子Common cis-acting element in promoter and enhancer regions
ARE	2	1	厌氧诱导cis-Acting regulatory element essential for the anaerobic induction
circadian	1	1	昼夜节律控制cis-Acting regulatory element involved in circadian control
TC-rich repeats	1	1	防御和应激cis-Acting element involved in defense and stress responsiveness
LTR	1	1	低温响应cis-Acting element involved in low-temperature responsiveness
O2-site	1	1	玉米醇溶酶蛋白代谢调节cis-Acting regulatory element involved in zein metabolism regulation
TATA-box	51	29	核心启动子Core promoter element around -30 of transcription start
TCA-element	1	1	水杨酸响应cis-Acting element involved in salicylic acid responsiveness
ABRE	0	1	脱落酸响应cis-Acting element involved in the abscisic acid responsiveness
P-box	0	1	赤霉素响应Gibberellin-responsive element
CGTCA-motif	1	1	茉莉酸甲酯响应cis-Acting regulatory element involved in the MeJA-responsiveness
TGACG-motif	1	1	茉莉酸甲酯响应cis-Acting regulatory element involved in the MeJA-responsiveness

cis-Element	垂枝Weeping	直枝 Upright	功能 Function
GATA-motif	1	1	光响应Part of a light responsive element
I-box	2	2	光响应Part of a light responsive element
AAAC-motif	1	0	光响应Light responsive element
GT1-motif	2	0	光响应Light responsive element
G-box	0	1	光响应cis-Acting regulatory element involved in light responsiveness
TCCC-motif	0	1	光响应Part of a light responsive element
CAAT-box	29	24	增强子Common cis-acting element in promoter and enhancer regions
ARE	2	1	厌氧诱导cis-Acting regulatory element essential for the anaerobic induction
circadian	1	1	昼夜节律控制cis-Acting regulatory element involved in circadian control
TC-rich repeats	1	1	防御和应激cis-Acting element involved in defense and stress responsiveness
LTR	1	1	低温响应cis-Acting element involved in low-temperature responsiveness
O2-site	1	1	玉米醇溶酶蛋白代谢调节cis-Acting regulatory element involved in zein metabolism regulation
TATA-box	51	29	核心启动子Core promoter element around -30 of transcription start
TCA-element	1	1	水杨酸响应cis-Acting element involved in salicylic acid responsiveness
ABRE	0	1	脱落酸响应cis-Acting element involved in the abscisic acid responsiveness
P-box	0	1	赤霉素响应Gibberellin-responsive element
CGTCA-motif	1	1	茉莉酸甲酯响应cis-Acting regulatory element involved in the MeJA-responsiveness
TGACG-motif	1	1	茉莉酸甲酯响应cis-Acting regulatory element involved in the MeJA-responsiveness

Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume

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