Promoting Effects of Uniconazole on Grafting Formation of Tomato Seedlings and Underlying Mechanisms

doi:10.16420/j.issn.0513-353x.2021-0306

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1275-1289.doi: 10.16420/j.issn.0513-353x.2021-0306

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Promoting Effects of Uniconazole on Grafting Formation of Tomato Seedlings and Underlying Mechanisms

MENG Xianmin¹^,², CUI Qingqing¹, DUAN Yundan¹, ZHUANG Tuanjie¹, PU Dan¹, DONG Chunjuan¹, YANG Wencai², SHANG Qingmao¹^,^*()

¹Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
²Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,Department of Vegetable Science,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China

Received:2021-11-30 Revised:2022-02-18 Online:2022-06-25 Published:2022-07-04
Contact: SHANG Qingmao E-mail:shangqingmao@caas.cn

Abstract

Abstract:

Uniconazole（S3307）,a gibberellin inhibitor,is often used to regulate plant growth and development,however,its effect on graft formation remains unclear. Here,tomato scion‘Yingfen 8’and stock‘Zhenai 1’were foliar sprayed twice with 0.25 mg · L^-1 uniconazole on 15 d and 21 d after sowing. The results showed that uniconazole inhibited the growth of scion and stock seedlings,the plant height decreased by 20.58% and 12.75% respectively compared with the control with deionized water at 27 d after sowing,that is 0 h after grafting;from 12 h to 72 h after grafting,uniconazole reduced the contents of GA₁₅ and GA₈,promoted the accumulation of GA₃,IAA,MEIAA,ICAld,TRP and iP7G;and it increased the expression difference in IAA1 above and below the graft union,GA20ox1,IAA1,and ARR17 expression was up-regulated above the graft union,while GA20ox1 and ARR17 expression were down-regulated below. From 6 h to 168 h after grafting,uniconazole also increased the relative expression of TMO6 and CyclinB1;2 both above and below the graft union,and WIND1,WOX4 and VND7 expression above;uniform expression of IAA1,ARR17,WIND1,TMO6,WOX4 and VND7 above and below the graft union occurred earlier,and xylem and phloem of the graft union were connected in advance. In addition,the graft union of uniconazole began to connect at 72 h after grafting,the connection between xylem and phloem was basically completed at 96-120 h after grafting,which was 24 h earlier than control. These results suggested that foliar sprayed uniconazole before grafting promoted tomato graft formation by regulating endogenous hormone levels in the graft union.

Key words: tomato, uniconazole, hormone, graft formation

CLC Number:

S641.2

MENG Xianmin, CUI Qingqing, DUAN Yundan, ZHUANG Tuanjie, PU Dan, DONG Chunjuan, YANG Wencai, SHANG Qingmao. Promoting Effects of Uniconazole on Grafting Formation of Tomato Seedlings and Underlying Mechanisms[J]. Acta Horticulturae Sinica, 2022, 49(6): 1275-1289.

Figures/Tables 7

Table 1 Primer sequences

基因名称 Gene	引物序列（5′-3′） Primer sequence
GA20ox1	F：TGGACGATGAATGGCGTTCC;R：TACCGCTCTGTGTAGGCAAC
IAA1	F：TGGATGGTGCCCCTTATCTA;R：ACAAGAAGACATAAACATTTCCCAA
ARABIDOPSIS RESPONSE REGULATOR17,ARR17	F：TGGACGATGAATGGCGTTCC;R：TACCGCTCTGTGTAGGCAAC
WOUND INDUCED DEDIFFERENTIATION1,WIND1	F：AACTGGTACTGCCAACTCCG;R：AGTCCAAGCGTGAACCCAAG
TARGET of MONOPTEROS6,TMO6	F：AAGACGTGTAGGAGGTACTGGA;R：GATAAATTCGGTATCTGCGGCG
WUSCHEL-RELATED HOMEOBOX4,WOX4	F：GTGGAATCACACCCAGGAGG;R：TATTTGTTGCGCGTTGGGTG
Cyclin B1;2	F：GGAAAGCCGCTTCCTCAAGT;R：GCTCCGTTAGCAACAATCGG
VASCULAR-RELATED NAC-DOMAIN7,VND7	F：TAGGACCAACCGAGCCACT;R：TCCGCATTCCGATGACACT
NAC DOMAIN-CONTAINING PROTEIN20,NAC020	F：TGGACGATGAATGGCGTTCC;R：TACCGCTCTGTGTAGGCAAC
Actin	F：AACAACGCCTCTTTCTTCTCTCT;R：AAAGAGATCCACAACCACTGTCT

Fig. 1 Exogenous uniconazole inhibited the growth of tomato scion and stock seedlings * indicates the significance of 0.05 level difference,IBM SPSS Statistics 20.0 software was used for independent-sample T-test. The same below.

Fig. 2 Exogenous uniconazole promoted graft formation process of tomato graft union Sc：Scion;St：Stock;pc：Parenchyma cell;co：Cortex;vb：Vascular bundle;ph：Phloem;x：Xylem;vc：Vascular bundle connection;The middle area of two red dashes is graft union.

Fig. 3 Exogenous uniconazole promoted xylem and phloem connection of tomato graft union A indicates xylem reconnection,which shows the hand-cut transverse section of 1 cm above the graft union（scion）,and observed the absorption content of acid fuchsin under stereomicroscope. B indicates phloem reconnection,the hand-cut transverse section of 1 cm below the graft union（stock）,and observed the fluorescence intensity under the fluorescence imaging microscope.

Fig. 4 Exogenous uniconazole promoted xylem and phloem connection of tomato graft union A indicates xylem reconnection,and observed the absorption content of acid fuchsin under stereomicroscope. B indicates phloem reconnection,the hand-cut transverse section of 1 cm below the graft union（stock）,and observed the fluorescence intensity under the fluorescence imaging microscope. * P < 0.05.

Fig. 5 Dynamic changes in hormone contents of tomato graft union GA15,GA8,GA3 and GA5 were gibberellin A15,gibberellin A8,gibberellin A3 and gibberellin A5,some GA8 data are not detected because the levels of the hormones in the samples were below the detection limit of the instrument;IAA,MEIAA,ICAld and TRP were indole-3-acetic acid,methyl indole-3-acetate,indole-3-carboxaldehyde and L-tryptophan;iP7G,cZR,tZOG were N6-Isopentenyl-adenine-7-glucoside,cis-Zeatin riboside,trans-zeatin-O-glucoside and trans-zeatin riboside,respectively.

Fig. 6 Relative expression of the hormone and graft formation associated gene in the graft union of tomato

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Promoting Effects of Uniconazole on Grafting Formation of Tomato Seedlings and Underlying Mechanisms

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