CsSWEET2a Promotes the Infection of Xanthomonas citri subsp. citri

doi:10.16420/j.issn.0513-353x.2021-0323

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1247-1260.doi: 10.16420/j.issn.0513-353x.2021-0323

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CsSWEET2a Promotes the Infection of Xanthomonas citri subsp. citri

MA Mingying¹^,², HAO Chenxing¹^,², ZHANG Kai¹^,², XIAO Guihua¹^,², SU Hanying¹^,², WEN Kang¹, DENG Ziniu¹^,², MA Xianfeng¹^,²^,^*()

¹National Center for Citrus Improvement Changsha,College of Horticulture,Hunan Agricultural University, Changsha 410128,China
²Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Changsha 410128,China

Received:2021-12-10 Revised:2022-02-28 Online:2022-06-25 Published:2022-07-04
Contact: MA Xianfeng E-mail:maxf8006@126.com

Abstract

Abstract:

After the infection of citrus canker pathogen Xanthomonas citri subsp. citri（Xcc）,infected the leaves of the susceptible germplasm‘Bingtang’Sweet Orange,it formed typical crater bulge symptoms at the infected site,but gradually showed brown necrosis at the infected site of the leaves of the resistant germplasm Citron C-05,without typical symptoms of canker disease. Based on the transcriptome sequencing analysis of‘Bingtang’Sweet Orange and Citron C-05. leaves inoculated with Xcc,the expression of 20 CsSWEET induced by Xcc was compared. The results showed that SWEET2a and SWEET17d were up-regulated by Xcc in‘Bingtang’Sweet Orange,and SWEET12b was up-regulated by Xcc in Citron C-05;qRT-PCR verification only SWEET2a induced by Xcc high expression in ‘Bingtang’Sweet Orange and no significant change in Citron C-05,The amino acids sequence similarity of SWEET2a in different citrus germplasm homologs was 94.6%. The cis-acting elements of the promoter in‘Bingtang’Sweet Orange and Citron C-05 were significantly different in types and quantities. The transient overexpression of SWEET2a in‘Bingtang’Sweet Orange and Citron C-05 showed that SWEET2a promoted the reproduction of Xcc,and the transgenic Arabidopsis thaliana lines inoculated with the virulence strain Pst.DC3000（Pseudomonas syringae tomato DC3000）was significantly higher than that of the wild type. The SWEET2a protein is partially localized to the plasma membrane,suggesting that SWEET2a may be hijacked by Xcc for transmembrane transport of sugar extracellular to supply Xcc with energy needed for intercellular colonization.

Key words: citrus, Xcc, Citron C-05, SWEET2a, gene function

CLC Number:

S666

MA Mingying, HAO Chenxing, ZHANG Kai, XIAO Guihua, SU Hanying, WEN Kang, DENG Ziniu, MA Xianfeng. CsSWEET2a Promotes the Infection of Xanthomonas citri subsp. citri[J]. Acta Horticulturae Sinica, 2022, 49(6): 1247-1260.

Figures/Tables 11

Table 1 The List of Primers

基因 Gene	引物名称 Primer ID	引物序列（5′-3′） Primer sequence	退火温度/℃ T_m	产物大小/ bp Size
SWEET17d	orange1.1t04897-qPCR-F1	AATTTCATTTTTGGGCTTCTAGGCA	58	105
SWEET17d	orange1.1t04897-qPCR-R1	AAATTCCTCCGTTGATCTACGCTGT
SWEET12b	orange1.1t02627-qPCR-F1	CACGTCTTCGTCTTCTCGGA	58	89
SWEET12b	orange1.1t02627-qPCR-R1	GCGTACGACCAGCCTCATAA
SWEET2a	Cs2g04140-qPCR-F1	TCGCTACAGTGAATTCAGTTGGAGC	60	174
SWEET2a	Cs2g04140-qPCR-R1	GCCGCCCATTGGAGTCAA
EF1a		GTAACCAAGTCTGCTGCCAAG
EF1a		GACCCAAACACCCAACACATT
SWEET2a	Cs2g04140-KpnⅠ-F	GGGGTACCATGTCTTCAGTTGGGATTTC	60	1 771
SWEET2a	Cs2g04140- KpnⅠ-R	CCGGTACCTGCAAATGAATCGATCAG
	Cs2g04140-F	TCTGGAACTCAAGCATTCCGACTTT	55	581
	p1300-eYFP-R	CCGGACACGCTGAACTTGT

Fig. 1 Symptoms of Xcc and Xoo inoculation in‘Bingtang’Sweet Orange and Citron C-05

Fig. 2 Differential expression analysis of SWEET family in ‘Bingtang’ Sweet Orange and Citron C-05 induced by Xcc

Fig. 3 Relative expression of SWEETs induced by Xcc and Xoo in‘Bingtang’Sweet Orange and Citron C-05 Two-tailed student’s t-test（* P < 0.05）.

Fig. 4 Sequence difference alignment of amino acids encoded by SWEET2a BTC：‘Bingtang’ Sweet Orange;JY C-05：Citron C-5;CGXY：Changguo Citron;Cg：Citrus Grandis;SJG：Fortunella Hindsii;PTJY：Common Citron.

Fig. 5 Sequence difference alignment of amino acids encoded by SWEET2a in different Citron medica JY C-05：Citron C-5;AGXY：Aiguo Citron;PTJY：Common Citron;YXY：Round Citron;CGXY：Changguo Citron;DNXY：Danna Citron;MGJY：American Citron;NCXY：Nanchuan Citron;YSXY：Wild Citron;XXY：Small Citron.

Table 2 The Cis-acting regulatory elements in promoter of SWEET2a

顺式元件 cis-Element	功能预测 Function prediction	序列 Sequence	起始位/bp Start position
顺式元件 cis-Element	功能预测 Function prediction	序列 Sequence	冰糖橙 ‘Bingtang’Sweet Orange	枸橼C-05 Citron C-05
ARE	厌氧诱导Anaerobic induction	AAACCA	+ 212	-905
			+ 1 046	-933
			+ 1 489
TCA-element	水杨酸响应Salicylic acid responsiveness	TCAGAAGAGG	+ 1 924
MBS	MYB结合位点参与干旱诱导	CAACTG	-1 626	+ 181
	MYB binding site involved in drought-inducibility			-611
				-1 422
LTR	低温诱导Low-temperature responsiveness	CCGAAA	-649	-623
CAT-box	分生组织表达Meristem expression	GCCACT	+ 198	+ 1 571
			-1 155
			+ 1 768
TC-rich repeats	防御和应激反应Defense and stress responsiveness	GTTTTCTTAC	+ 1 478
ABRE	脱落酸响应Abscisic acid responsiveness	ACGTG	+ 185
			+ 186
			-681
			+ 855
			-1 122
			-1 124
			+ 1 125
			-1 221
CCAAT-box	MYBHv1结合位点MYBHv1 binding site	CAACGG	-1 500	-1 067
O2-site	Zein新陈代谢调节Zein metabolism regulation	GATGATGTGG		-356
O2-site		GATGATGTGG		+ 399
GCN4_motif	胚乳表达Endosperm expression	TGAGTCA		+ 44
TGACG-motif	茉莉酸甲酯响应MeJA-responsiveness	TGACG		-1 419
TATC-box	赤霉素响应Gibberellin responsiveness	TATCCCA		-341

Fig. 6 The expression patten of SWEET2a in Nicotiana benthamiana A：The Nicotiana benthamiana leaf containing 35S::SWEET2a:eYFP was injected;B：After injection of 35S::SWEET2a:eYFP and cytoplasmic membrane localization gene PM-RB,the plasma wall of Nicotiana benthamiana leaf was isolated. The yellow arrow indicates the fluorescence expression of SWEET2a;The blue arrow indicates the location of the cell wall;The white arrow indicates the fluorescence expression of PM-RB;The red arrows indicate merge expression of the two.

Fig. 7 The symptom of Xcc in leaves of‘Bingtang’Sweet Orange and Citron C-05 after transient overexpression of SWEET2a at 15 d（A）and the growth of Xcc at 0-4 d（B） A：The symptoms of transient overexpression of SWEET2a in‘Bingtang’Sweet Orange leaves in 15 d;B：The growth of Xcc in‘Bingtang’ Sweet Orange after transient overexpression. Two-tailed Student’s t-test（* P < 0.05）.

Fig. 8 Verification of T1 generation transgenic plants OE-SWEET2a in Arabidopsis thaliana

Fig. 9 Symptoms（A）and pathogen content（B）of OE-SWEET2a at 3 days after inoculation with Pst.DC3000 A：Symptoms of Pst. DC3000 in 3 d;B：Pst. DC3000 content per unit leaf area;WT：Wild Type. Two-tailed Student’s t-test（* P < 0.05）.

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CsSWEET2a Promotes the Infection of Xanthomonas citri subsp. citri

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