Genomic Identification and Molecular Evolution of the WRKY Family in Nymphaea colorata

doi:10.16420/j.issn.0513-353x.2021-0092

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1121-1135.doi: 10.16420/j.issn.0513-353x.2021-0092

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Genomic Identification and Molecular Evolution of the WRKY Family in Nymphaea colorata

PAN Xinfeng, YE Fangting, MAO Zhijun, LI Zhaowei, FAN Kai()

Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilzation of Crops,College of Agriculture,Fujian Agriculture And Forestry University,Fuzhou 350002,China

Received:2021-12-06 Revised:2022-02-11 Online:2022-05-25 Published:2022-05-25
Contact: FAN Kai E-mail:fankai@fafu.edu.cn

Abstract

Abstract:

Sixty-nine NcWRKY transcription factors were identified in Nymphaea colorata and can be divided into three large subfamilies（I,Ⅱ and Ⅲ）. The Ⅱ subfamily can be further classified into Ⅱa,Ⅱb,Ⅱc,Ⅱd and Ⅱe. The Ⅱc as subfamily has the highest number of NcWRKY members with 17 members. Random coil accounted for 39%-78% in the protein secondary structure of the NcWRKY members. Motif 1 and motif 3 were annotated as WRKY conserved domain. NcWRKY members were distributed in waterlily 14 chromosomes. All members of the Ⅱa subfamily were located on chromosome Nc4. NcWRKY members can be regulated by hormones through the cis-element analysis of their promoters. NcWRKY04 and NcWRKY18 had very high expression level in mature leafstalk,NcWRKY05 had very high expression level in flower and NcWRKY61 had very high expression level in stamen through tissue specific expression analysis. NcWRKY61 and NcWRKY63 may have a co-expression relationship with other members through the gene expression network.

Key words: Nymphaea colorata, WRKY, genomic identification, molecular evolution

CLC Number:

S682.32

PAN Xinfeng, YE Fangting, MAO Zhijun, LI Zhaowei, FAN Kai. Genomic Identification and Molecular Evolution of the WRKY Family in Nymphaea colorata[J]. Acta Horticulturae Sinica, 2022, 49(5): 1121-1135.

Figures/Tables 9

References 49

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名称 Name	位点 Locus	亚家族 Subfamily	蛋白质长度/aa Protein length	分子量 Molecular Weight	pI	α-螺旋 α-helix	延伸链 Extended strand	β-折叠 β-turn	无规则卷曲 Random coil
NcWRKY01	GWHPAAYW001268	Ⅱd	299	33 359.08	9.69	67	29	12	191
NcWRKY02	GWHPAAYW001468	Ⅲ	219	24 178.36	9.1	89	28	4	98
NcWRKY03	GWHPAAYW002553	Ⅱc	262	28 583.02	8.78	61	45	25	131
NcWRKY04	GWHPAAYW003267	Ⅲ	350	39 563.96	5.07	78	37	14	221
NcWRKY05	GWHPAAYW011370	Ⅱc	306	33 839.58	6.31	74	31	17	184
NcWRKY06	GWHPAAYW012017	Ⅰ	832	88 126.14	5.42	75	83	38	636
NcWRKY07	GWHPAAYW012284	Ⅱc	224	25 222.62	9.04	62	35	13	114
NcWRKY08	GWHPAAYW012407	Ⅲ	194	21 868.50	7.67	51	43	12	88
NcWRKY09	GWHPAAYW012566	Ⅰ	313	34 228.55	10.01	50	36	9	218
NcWRKY10	GWHPAAYW013009	Ⅰ	316	34 251.61	9.3	48	60	14	194
NcWRKY11	GWHPAAYW013286	Ⅱe	345	37 142.72	5.01	112	34	29	170
NcWRKY12	GWHPAAYW013314	Ⅱc	208	23 139.17	8.94	59	26	14	109
NcWRKY13	GWHPAAYW013362	Ⅰ	181	19 803.13	7.8	27	41	4	109
NcWRKY14	GWHPAAYW014542	Ⅰ	816	87 750.28	5.25	92	95	28	601
NcWRKY15	GWHPAAYW015340	Ⅱd	361	38 817.12	9.45	66	41	33	221
NcWRKY16	GWHPAAYW016607	Ⅱd	337	36 359.40	9.68	83	35	26	193
NcWRKY17	GWHPAAYW017157	Ⅱa	293	31 896.80	8.52	85	40	13	155
NcWRKY18	GWHPAAYW017158	Ⅱa	315	33 882.86	6.62	114	45	13	143
NcWRKY19	GWHPAAYW017159	Ⅱa	425	46 389.46	9.37	159	51	15	200
NcWRKY20	GWHPAAYW017160	Ⅱa	315	33 832.56	6.61	88	45	10	172
NcWRKY21	GWHPAAYW017161	Ⅱa	327	35 972.96	6.32	109	39	12	167
NcWRKY22	GWHPAAYW017408	Ⅰ	1 209	13 726.21	8.92	382	238	107	482
NcWRKY23	GWHPAAYW018263	Ⅱc	236	26 890.16	9.42	30	59	11	136
NcWRKY24	GWHPAAYW018963	Ⅱc	325	35 439.54	5.19	76	45	12	192
NcWRKY25	GWHPAAYW019317	Ⅰ	475	51 676.63	9.42	56	53	22	344
NcWRKY26	GWHPAAYW019476	Ⅱc	292	32 449.26	6.84	80	37	15	160
NcWRKY27	GWHPAAYW020087	Ⅱd	85	9 747.24	9.88	13	24	0	42
NcWRKY28	GWHPAAYW020328	Ⅲ	301	33 205.85	4.87	91	44	15	151
NcWRKY29	GWHPAAYW020329	Ⅲ	287	31 497.25	5.43	104	22	10	151
NcWRKY30	GWHPAAYW020535	Ⅱc	326	35 958.21	9.38	85	40	14	187
NcWRKY31	GWHPAAYW020992	Ⅱb	522	56 768.49	8.47	157	51	13	301
NcWRKY32	GWHPAAYW020993	Ⅱb	550	59 854.45	6.3	128	60	19	343
NcWRKY33	GWHPAAYW020994	Ⅱb	483	52 474.26	6.43	140	36	13	294
NcWRKY34	GWHPAAYW021005	Ⅱc	390	44 581.80	5.73	67	50	26	247
NcWRKY35	GWHPAAYW021006	Ⅱc	207	23 478.24	5.57	40	38	18	111
NcWRKY36	GWHPAAYW021007	Ⅱc	336	38 210.70	7.54	39	58	29	210
NcWRKY37	GWHPAAYW021021	Ⅱc	252	27 873.25	8.78	73	42	42	117
NcWRKY38	GWHPAAYW021561	Ⅱd	300	33 420.36	9.71	77	31	13	179
NcWRKY39	GWHPAAYW023351	Ⅰ	653	69 898.00	5.94	64	75	21	493
NcWRKY40	GWHPAAYW023893	Ⅱb	536	57 654.27	6.48	149	44	16	327
NcWRKY41	GWHPAAYW024187	Ⅱc	194	22 010.58	8.97	57	24	11	102
NcWRKY42	GWHPAAYW024283	Ⅰ	614	66 224.72	6.19	79	73	31	431
NcWRKY43	GWHPAAYW024356	Ⅰ	207	22 640.31	7.08	32	41	6	128
NcWRKY44	GWHPAAYW024753	Ⅰ	541	57 521.59	7.34	55	70	26	390
NcWRKY45	GWHPAAYW025120	Ⅰ	376	40 730.26	6.54	36	55	15	270
NcWRKY46	GWHPAAYW025126	Ⅰ	314	34 201.39	8.97	38	41	14	221
NcWRKY47	GWHPAAYW025139	Ⅰ	231	25 176.13	6.06	45	47	16	123
NcWRKY48	GWHPAAYW025454	Ⅱb	496	53 858.63	5.15	126	56	23	291
NcWRKY49	GWHPAAYW025832	Ⅰ	703	75 743.55	5.72	102	65	23	513
名称 Name	位点 Locus	亚家族 Subfamily	蛋白质长度/aa Protein length	分子量 Molecular Weight	pI	α-螺旋 α-helix	延伸链 Extended strand	β-折叠 β-turn	无规则卷曲 Random coil
NcWRKY50	GWHPAAYW026703	Ⅱe	94	10 236.55	9.99	22	19	9	44
NcWRKY51	GWHPAAYW027586	Ⅱd	289	31 308.83	9.85	58	26	12	193
NcWRKY52	GWHPAAYW027773	Ⅲ	366	40 600.28	5.83	92	37	10	227
NcWRKY53	GWHPAAYW004306	Ⅱb	473	51 009.43	5.11	153	61	20	239
NcWRKY54	GWHPAAYW005049	Ⅱb	540	57 498.21	6.36	134	57	18	331
NcWRKY55	GWHPAAYW005095	Ⅰ	605	65 201.75	7.75	57	59	12	477
NcWRKY56	GWHPAAYW005338	Ⅲ	263	29 278.76	5.46	86	26	14	137
NcWRKY57	GWHPAAYW005461	Ⅱe	255	28 218.34	5.41	41	35	12	167
NcWRKY58	GWHPAAYW005491	Ⅱc	315	34 590.63	6.27	80	27	8	200
NcWRKY59	GWHPAAYW006427	Ⅱc	317	35 052.79	6.42	57	30	11	219
NcWRKY60	GWHPAAYW006549	Ⅱe	431	47 359.75	5.77	78	32	19	302
NcWRKY61	GWHPAAYW007012	Ⅱb	557	59 663.54	6.11	156	39	11	351
NcWRKY62	GWHPAAYW008691	Ⅱd	1 144	126 555.82	8.82	383	213	90	458
NcWRKY63	GWHPAAYW009733	Ⅱd	73	8 382.87	9.82	9	15	4	45
NcWRKY64	GWHPAAYW009825	Ⅱe	122	13 701.70	10.19	24	16	12	70
NcWRKY65	GWHPAAYW009961	Ⅱc	199	21 660.89	6.94	46	27	6	120
NcWRKY66	GWHPAAYW009962	Ⅱe	290	31 222.80	5.11	72	19	11	188
NcWRKY67	GWHPAAYW010546	Ⅱc	230	25 695.79	6.59	47	40	9	134
NcWRKY68	GWHPAAYW010567	Ⅱb	1 076	114 597.89	5.94	404	153	63	456
NcWRKY69	GWHPAAYW010914	Ⅱe	445	47 724.61	5.12	87	35	21	302

名称 Name	位点 Locus	亚家族 Subfamily	蛋白质长度/aa Protein length	分子量 Molecular Weight	pI	α-螺旋 α-helix	延伸链 Extended strand	β-折叠 β-turn	无规则卷曲 Random coil
NcWRKY01	GWHPAAYW001268	Ⅱd	299	33 359.08	9.69	67	29	12	191
NcWRKY02	GWHPAAYW001468	Ⅲ	219	24 178.36	9.1	89	28	4	98
NcWRKY03	GWHPAAYW002553	Ⅱc	262	28 583.02	8.78	61	45	25	131
NcWRKY04	GWHPAAYW003267	Ⅲ	350	39 563.96	5.07	78	37	14	221
NcWRKY05	GWHPAAYW011370	Ⅱc	306	33 839.58	6.31	74	31	17	184
NcWRKY06	GWHPAAYW012017	Ⅰ	832	88 126.14	5.42	75	83	38	636
NcWRKY07	GWHPAAYW012284	Ⅱc	224	25 222.62	9.04	62	35	13	114
NcWRKY08	GWHPAAYW012407	Ⅲ	194	21 868.50	7.67	51	43	12	88
NcWRKY09	GWHPAAYW012566	Ⅰ	313	34 228.55	10.01	50	36	9	218
NcWRKY10	GWHPAAYW013009	Ⅰ	316	34 251.61	9.3	48	60	14	194
NcWRKY11	GWHPAAYW013286	Ⅱe	345	37 142.72	5.01	112	34	29	170
NcWRKY12	GWHPAAYW013314	Ⅱc	208	23 139.17	8.94	59	26	14	109
NcWRKY13	GWHPAAYW013362	Ⅰ	181	19 803.13	7.8	27	41	4	109
NcWRKY14	GWHPAAYW014542	Ⅰ	816	87 750.28	5.25	92	95	28	601
NcWRKY15	GWHPAAYW015340	Ⅱd	361	38 817.12	9.45	66	41	33	221
NcWRKY16	GWHPAAYW016607	Ⅱd	337	36 359.40	9.68	83	35	26	193
NcWRKY17	GWHPAAYW017157	Ⅱa	293	31 896.80	8.52	85	40	13	155
NcWRKY18	GWHPAAYW017158	Ⅱa	315	33 882.86	6.62	114	45	13	143
NcWRKY19	GWHPAAYW017159	Ⅱa	425	46 389.46	9.37	159	51	15	200
NcWRKY20	GWHPAAYW017160	Ⅱa	315	33 832.56	6.61	88	45	10	172
NcWRKY21	GWHPAAYW017161	Ⅱa	327	35 972.96	6.32	109	39	12	167
NcWRKY22	GWHPAAYW017408	Ⅰ	1 209	13 726.21	8.92	382	238	107	482
NcWRKY23	GWHPAAYW018263	Ⅱc	236	26 890.16	9.42	30	59	11	136
NcWRKY24	GWHPAAYW018963	Ⅱc	325	35 439.54	5.19	76	45	12	192
NcWRKY25	GWHPAAYW019317	Ⅰ	475	51 676.63	9.42	56	53	22	344
NcWRKY26	GWHPAAYW019476	Ⅱc	292	32 449.26	6.84	80	37	15	160
NcWRKY27	GWHPAAYW020087	Ⅱd	85	9 747.24	9.88	13	24	0	42
NcWRKY28	GWHPAAYW020328	Ⅲ	301	33 205.85	4.87	91	44	15	151
NcWRKY29	GWHPAAYW020329	Ⅲ	287	31 497.25	5.43	104	22	10	151
NcWRKY30	GWHPAAYW020535	Ⅱc	326	35 958.21	9.38	85	40	14	187
NcWRKY31	GWHPAAYW020992	Ⅱb	522	56 768.49	8.47	157	51	13	301
NcWRKY32	GWHPAAYW020993	Ⅱb	550	59 854.45	6.3	128	60	19	343
NcWRKY33	GWHPAAYW020994	Ⅱb	483	52 474.26	6.43	140	36	13	294
NcWRKY34	GWHPAAYW021005	Ⅱc	390	44 581.80	5.73	67	50	26	247
NcWRKY35	GWHPAAYW021006	Ⅱc	207	23 478.24	5.57	40	38	18	111
NcWRKY36	GWHPAAYW021007	Ⅱc	336	38 210.70	7.54	39	58	29	210
NcWRKY37	GWHPAAYW021021	Ⅱc	252	27 873.25	8.78	73	42	42	117
NcWRKY38	GWHPAAYW021561	Ⅱd	300	33 420.36	9.71	77	31	13	179
NcWRKY39	GWHPAAYW023351	Ⅰ	653	69 898.00	5.94	64	75	21	493
NcWRKY40	GWHPAAYW023893	Ⅱb	536	57 654.27	6.48	149	44	16	327
NcWRKY41	GWHPAAYW024187	Ⅱc	194	22 010.58	8.97	57	24	11	102
NcWRKY42	GWHPAAYW024283	Ⅰ	614	66 224.72	6.19	79	73	31	431
NcWRKY43	GWHPAAYW024356	Ⅰ	207	22 640.31	7.08	32	41	6	128
NcWRKY44	GWHPAAYW024753	Ⅰ	541	57 521.59	7.34	55	70	26	390
NcWRKY45	GWHPAAYW025120	Ⅰ	376	40 730.26	6.54	36	55	15	270
NcWRKY46	GWHPAAYW025126	Ⅰ	314	34 201.39	8.97	38	41	14	221
NcWRKY47	GWHPAAYW025139	Ⅰ	231	25 176.13	6.06	45	47	16	123
NcWRKY48	GWHPAAYW025454	Ⅱb	496	53 858.63	5.15	126	56	23	291
NcWRKY49	GWHPAAYW025832	Ⅰ	703	75 743.55	5.72	102	65	23	513
名称 Name	位点 Locus	亚家族 Subfamily	蛋白质长度/aa Protein length	分子量 Molecular Weight	pI	α-螺旋 α-helix	延伸链 Extended strand	β-折叠 β-turn	无规则卷曲 Random coil
NcWRKY50	GWHPAAYW026703	Ⅱe	94	10 236.55	9.99	22	19	9	44
NcWRKY51	GWHPAAYW027586	Ⅱd	289	31 308.83	9.85	58	26	12	193
NcWRKY52	GWHPAAYW027773	Ⅲ	366	40 600.28	5.83	92	37	10	227
NcWRKY53	GWHPAAYW004306	Ⅱb	473	51 009.43	5.11	153	61	20	239
NcWRKY54	GWHPAAYW005049	Ⅱb	540	57 498.21	6.36	134	57	18	331
NcWRKY55	GWHPAAYW005095	Ⅰ	605	65 201.75	7.75	57	59	12	477
NcWRKY56	GWHPAAYW005338	Ⅲ	263	29 278.76	5.46	86	26	14	137
NcWRKY57	GWHPAAYW005461	Ⅱe	255	28 218.34	5.41	41	35	12	167
NcWRKY58	GWHPAAYW005491	Ⅱc	315	34 590.63	6.27	80	27	8	200
NcWRKY59	GWHPAAYW006427	Ⅱc	317	35 052.79	6.42	57	30	11	219
NcWRKY60	GWHPAAYW006549	Ⅱe	431	47 359.75	5.77	78	32	19	302
NcWRKY61	GWHPAAYW007012	Ⅱb	557	59 663.54	6.11	156	39	11	351
NcWRKY62	GWHPAAYW008691	Ⅱd	1 144	126 555.82	8.82	383	213	90	458
NcWRKY63	GWHPAAYW009733	Ⅱd	73	8 382.87	9.82	9	15	4	45
NcWRKY64	GWHPAAYW009825	Ⅱe	122	13 701.70	10.19	24	16	12	70
NcWRKY65	GWHPAAYW009961	Ⅱc	199	21 660.89	6.94	46	27	6	120
NcWRKY66	GWHPAAYW009962	Ⅱe	290	31 222.80	5.11	72	19	11	188
NcWRKY67	GWHPAAYW010546	Ⅱc	230	25 695.79	6.59	47	40	9	134
NcWRKY68	GWHPAAYW010567	Ⅱb	1 076	114 597.89	5.94	404	153	63	456
NcWRKY69	GWHPAAYW010914	Ⅱe	445	47 724.61	5.12	87	35	21	302

ID	序列	长度/aa
ID	Sequence	Length
motif 1	ILDDGYRWRKYGQKVVKGNPYPRSYYRCT	29
motif 2	PGCPVRKQVZRASEDPSIVITTYEGKHNH	29
motif 3	RPSEDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCQVKKKVERSHDGLVTEIIYKGTHNHPKPQPSRR	67
motif 4	NTNMQVSLNPVMLMPPNQIEVNRALPYSRGPDAVSLDLGVGISQAAENSSLHKQNQAEADSMLRQVHGGNAPSSGINCPVQSAGILPFYGNGIMGNRPDHYRVKEEDGQNFNF	113
motif 5	EREPRVAFQTMSEVD	15
motif 6	LKAEVGRVKEENERLKRMLSRICKSYKALQMQFASLIQS	39
motif 7	AAAVASAJASDPSFTAALAAAJSSIIG	27
motif 8	PLPVQATAMASTTSAAASMLLSGSSSST	28
motif 9	DVPTSSSTNRETSAPMAVQNL	21
motif 10	GRCHCSKRRKSRVKRVIRVPAISSKLAD	28
motif 11	MEEVEEANKAAVECCHRLIAVLAQPQEGLEWKGVMGLTGEALMKFRKVVSLLNHKAGHARFRRGSTQSPPRQKICMENPKLGLDPAAPKVYPEPPQCPPHPQAPNPHFQFLQQMKLQADLLYRRSNSSINLKFDNSSCTPTMSSTRSFMSSLSMDGSVANMDGKGFCLIGPASSQDRISHQQKRKCSGKGEDG	193
motif 12	MSSLNNFHRGTGRFMLGSEVDASHAQQFT	29
motif 13	ECPYVIYYITPQHDCDVGVISAAQIPFVSHGAPCAGRQGFY	41
motif 14	LAEPSPTTGTFSFRPCNAGFNSGLVSNAFSSSWNVSKKHYLGDGSGPGSFVFKPREEASSSVGALAVVNSNPEKNQNFGQCQGYSEPQASIKIEAMAVSAKDLSLSVATSEAPACGAASNSITPANPSSDELNVRRGPDAETQHVSLDQKGAPLTVVE	158
motif 15	SDSLGDEGDEGEPESKRRKME	21
motif 16	VCTDLPAYTPEIFPAAENDSCFQLQDILDFCDPLIDGELFEGLEMDGNLQIPTSDSNQPNKIGDSQAKKQRGECSSRKRK	80
motif 17	QGRAFNQLEABGTPELSSLSTSDDEGDED	29
motif 18	DQAQQPHAKKARVSVRARCD	20
motif 19	SSSASISVSPSYPTITLDLTQTSSPPZF	28
motif 20	GSPVALSLVMLKPPNHIENGRAPLFPTASNVVSLDLGVGIG	41

ID	序列	长度/aa
ID	Sequence	Length
motif 1	ILDDGYRWRKYGQKVVKGNPYPRSYYRCT	29
motif 2	PGCPVRKQVZRASEDPSIVITTYEGKHNH	29
motif 3	RPSEDGYNWRKYGQKQVKGSEFPRSYYKCTHPNCQVKKKVERSHDGLVTEIIYKGTHNHPKPQPSRR	67
motif 4	NTNMQVSLNPVMLMPPNQIEVNRALPYSRGPDAVSLDLGVGISQAAENSSLHKQNQAEADSMLRQVHGGNAPSSGINCPVQSAGILPFYGNGIMGNRPDHYRVKEEDGQNFNF	113
motif 5	EREPRVAFQTMSEVD	15
motif 6	LKAEVGRVKEENERLKRMLSRICKSYKALQMQFASLIQS	39
motif 7	AAAVASAJASDPSFTAALAAAJSSIIG	27
motif 8	PLPVQATAMASTTSAAASMLLSGSSSST	28
motif 9	DVPTSSSTNRETSAPMAVQNL	21
motif 10	GRCHCSKRRKSRVKRVIRVPAISSKLAD	28
motif 11	MEEVEEANKAAVECCHRLIAVLAQPQEGLEWKGVMGLTGEALMKFRKVVSLLNHKAGHARFRRGSTQSPPRQKICMENPKLGLDPAAPKVYPEPPQCPPHPQAPNPHFQFLQQMKLQADLLYRRSNSSINLKFDNSSCTPTMSSTRSFMSSLSMDGSVANMDGKGFCLIGPASSQDRISHQQKRKCSGKGEDG	193
motif 12	MSSLNNFHRGTGRFMLGSEVDASHAQQFT	29
motif 13	ECPYVIYYITPQHDCDVGVISAAQIPFVSHGAPCAGRQGFY	41
motif 14	LAEPSPTTGTFSFRPCNAGFNSGLVSNAFSSSWNVSKKHYLGDGSGPGSFVFKPREEASSSVGALAVVNSNPEKNQNFGQCQGYSEPQASIKIEAMAVSAKDLSLSVATSEAPACGAASNSITPANPSSDELNVRRGPDAETQHVSLDQKGAPLTVVE	158
motif 15	SDSLGDEGDEGEPESKRRKME	21
motif 16	VCTDLPAYTPEIFPAAENDSCFQLQDILDFCDPLIDGELFEGLEMDGNLQIPTSDSNQPNKIGDSQAKKQRGECSSRKRK	80
motif 17	QGRAFNQLEABGTPELSSLSTSDDEGDED	29
motif 18	DQAQQPHAKKARVSVRARCD	20
motif 19	SSSASISVSPSYPTITLDLTQTSSPPZF	28
motif 20	GSPVALSLVMLKPPNHIENGRAPLFPTASNVVSLDLGVGIG	41

复制类型	复制基因1	复制基因2	亚家族	Ka	Ks	Ka/Ks
Duplicated type	Duplicated gene 1	Duplicated gene 2	Subfamily	Ka	Ks	Ka/Ks
片段复制	NcWRKY02	NcWRKY08	Ⅲ	0.6235	1.6690	0.3735
Segmental duplication	NcWRKY01	NcWRKY38	Ⅱd	0.0884	0.9775	0.0905
	NcWRKY03	NcWRKY37	Ⅱc	0.3228	2.0820	0.1549
	NcWRKY06	NcWRKY14	Ⅰ	0.2384	0.9139	0.2609
	NcWRKY09	NcWRKY25	Ⅰ	0.3501	1.0760	0.3252
	NcWRKY07	NcWRKY41	Ⅱc	0.2282	2.1670	0.1052
	NcWRKY05	NcWRKY59	Ⅱc	0.2031	1.0310	0.1969
	NcWRKY11	NcWRKY66	Ⅱe	0.2233	1.4330	0.1557
	NcWRKY12	NcWRKY65	Ⅱc	0.3091	1.8660	0.1655
	NcWRKY16	NcWRKY51	Ⅱd	0.1750	0.9797	0.1786
	NcWRKY30	NcWRKY58	Ⅱc	0.3375	0.9217	0.3662
	NcWRKY28	NcWRKY56	Ⅲ	0.3797	1.0670	0.3556
	NcWRKY31	NcWRKY68	Ⅱb	0.3438	1.4880	0.2309
	NcWRKY48	NcWRKY53	Ⅱb	0.2196	1.0670	0.2058
	NcWRKY50	NcWRKY64	Ⅱe	0.2693	1.3330	0.2020
串联复制 Tandem duplication	NcWRKY18	NcWRKY20	Ⅱa	0.1617	0.6405	0.2525

Genomic Identification and Molecular Evolution of the WRKY Family in Nymphaea colorata

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