Identification of Pigment Components and Key Genes in Carotenoid Pathway in Mutants of Chimeric Ananas comosus var. bracteatus

doi:10.16420/j.issn.0513-353x.2021-0355

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1081-1091.doi: 10.16420/j.issn.0513-353x.2021-0355

• Research Papers • Previous Articles Next Articles

Identification of Pigment Components and Key Genes in Carotenoid Pathway in Mutants of Chimeric Ananas comosus var. bracteatus

ZHOU Xuzixin, YANG Wei, MAO Meiqin, XUE Yanbin, MA Jun()

College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China

Received:2021-06-15 Revised:2021-09-07 Online:2022-05-25 Published:2022-05-25
Contact: MA Jun E-mail:junma365@hotmail.com

Abstract

Abstract:

In order to explore how the phenotype of yellow mutants of chimeric Ananas comosus var. bracteatus formed,chimeral leaves in red stage and yellow mutants were taken in this study for comprehensive and comparative analysis using CIEL^*a^*b^* coloration,pigment determination,histocytological observation,LC-MS/MS detection and illumina RNA-seq. Results of CIEL^*a^*b^* indicated that the color of the green central part and red margin part of the chimeric leaves was consisted of green-yellow and red-yellow color,respectively. The yellow mutant showed bright yellow color with extremely slight green. The significant decrease of total chlorophyll content,Chl.a/b ratio,total anthocyanin content and the significant increase of the proportion of carotenoid and Chl.b of the yellow mutant leaf resulted in the loss of green and red color and turned to yellow. The yellow pigments were scattered in a bit of mesophyll cells of the yellow mutant leaf while the other leaf cells were transparent and colorless. Lutein accounted for 79% of the 12 detected carotenoids content which is the dominant pigment of the yellow mutant leaf. The decrease of PSY1 expression and phytoene,carotenoid,lutein content indicated that PSY1 probably was a key gene responsible for the carotenoid biosynthesis limitation in the yellow mutant of chimeric Ananas comosus var. bracteatus.

Key words: Ananas comosus var. bracteatus, yellow mutant, carotenoid, metabolome, transcriptome

CLC Number:

S668.3

ZHOU Xuzixin, YANG Wei, MAO Meiqin, XUE Yanbin, MA Jun. Identification of Pigment Components and Key Genes in Carotenoid Pathway in Mutants of Chimeric Ananas comosus var. bracteatus[J]. Acta Horticulturae Sinica, 2022, 49(5): 1081-1091.

Figures/Tables 6

References 39

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样品 Sample	部位Position	L^*	a^*	b^*
金边红苞凤梨	中央绿色 Green central part	55.69 ± 1.95 c	-5.42 ± 0.25 c	17.23 ± 1.55 b
Acomosus comosus	边缘红色 Red margin part	67.62 ± 1.30 b	23.48 ± 2.89 a	17.52 ± 0.83 b
金黄色突变体Yellow mutant		72.61 ± 3.23 a	-0.30 ± 0.67 b	31.13 ± 0.54 a

样品 Sample	部位Position	L^*	a^*	b^*
金边红苞凤梨	中央绿色 Green central part	55.69 ± 1.95 c	-5.42 ± 0.25 c	17.23 ± 1.55 b
Acomosus comosus	边缘红色 Red margin part	67.62 ± 1.30 b	23.48 ± 2.89 a	17.52 ± 0.83 b
金黄色突变体Yellow mutant		72.61 ± 3.23 a	-0.30 ± 0.67 b	31.13 ± 0.54 a

分支 Branch	类胡萝卜素组分 Carotenoid	金边红苞凤梨Chimeric plant		金黄色突变体 Yellow
分支 Branch	类胡萝卜素组分 Carotenoid	中央绿色Green	边缘红色 Red	金黄色突变体 Yellow
LCYE	叶黄素Lutein	26.98 ± 0.06 a（74%）	23.37 ± 0.08 c（75%）	24.92 ± 0.13 b（79%）
	α-隐黄质α-cryptoxanthin	15.52 ± 0.08 b（< 1%）	14.20 ± 0.19 c（< 1%）	16.46 ± 0.31 a（< 1%）
	α-胡萝卜素α-carotene	20.52 ± 0.12 a（1%）	15.19 ± 0.14 b（< 1%）	16.90 ± 0.27 b（< 1%）
LCYB	β-胡萝卜素β-carotene	23.98 ± 0.03 a（10%）	19.57 ± 0.09 c（5%）	21.19 ± 0.11 b（6%）
	新黄质Neoxanthin	23.76 ± 0.04 a（9%）	18.59 ± 0.10 b（3%）	19.23 ± 0.10 b（2%）
	玉米黄质Zeaxanthin	21.21 ± 0.09 a（1%）	16.58 ± 0.09 b（1%）	16.98 ± 0.10 b（< 1%）
	紫黄质Violaxanthin	22.71 ± 0.15 a（4%）	20.77 ± 0.13 c（13%）	22.01 ± 0.11 b（11%）
	花药黄质Antheraxanthin	19.83 ± 0.14 a（1%）	15.55 ± 0.21 b（< 1%）	17.30 ± 0.11 b（< 1%）
其他Others	八氢番茄红素Phytoene	18.92 ± 0.03a（< 1%）	15.74 ± 0.12 b（< 1%）	15.65 ± 0.13 b（< 1%）
	六氢番茄红素Phytofluene	17.58 ± 0.07 b（< 1%）	17.91 ± 0.10 a（2%）	17.47 ± 0.20 b（1%）
	β-阿朴胡萝卜素醛β-apo-8'-carotenal	16.09 ± 0.04 a（< 1%）	13.47 ± 0.12 c（< 1%）	14.95 ± 0.18 b（< 1%）
	β-隐黄质β- cryptoxanthin	19.22 ± 0.04 a（< 1%）	16.52 ± 0.14 b（1%）	18.76 ± 0.48 a（1%）
	总量 Total	27.32 ± 0.05 a	23.78 ± 0.08 c	25.26 ± 0.11 b

分支 Branch	类胡萝卜素组分 Carotenoid	金边红苞凤梨Chimeric plant		金黄色突变体 Yellow
分支 Branch	类胡萝卜素组分 Carotenoid	中央绿色Green	边缘红色 Red	金黄色突变体 Yellow
LCYE	叶黄素Lutein	26.98 ± 0.06 a（74%）	23.37 ± 0.08 c（75%）	24.92 ± 0.13 b（79%）
	α-隐黄质α-cryptoxanthin	15.52 ± 0.08 b（< 1%）	14.20 ± 0.19 c（< 1%）	16.46 ± 0.31 a（< 1%）
	α-胡萝卜素α-carotene	20.52 ± 0.12 a（1%）	15.19 ± 0.14 b（< 1%）	16.90 ± 0.27 b（< 1%）
LCYB	β-胡萝卜素β-carotene	23.98 ± 0.03 a（10%）	19.57 ± 0.09 c（5%）	21.19 ± 0.11 b（6%）
	新黄质Neoxanthin	23.76 ± 0.04 a（9%）	18.59 ± 0.10 b（3%）	19.23 ± 0.10 b（2%）
	玉米黄质Zeaxanthin	21.21 ± 0.09 a（1%）	16.58 ± 0.09 b（1%）	16.98 ± 0.10 b（< 1%）
	紫黄质Violaxanthin	22.71 ± 0.15 a（4%）	20.77 ± 0.13 c（13%）	22.01 ± 0.11 b（11%）
	花药黄质Antheraxanthin	19.83 ± 0.14 a（1%）	15.55 ± 0.21 b（< 1%）	17.30 ± 0.11 b（< 1%）
其他Others	八氢番茄红素Phytoene	18.92 ± 0.03a（< 1%）	15.74 ± 0.12 b（< 1%）	15.65 ± 0.13 b（< 1%）
	六氢番茄红素Phytofluene	17.58 ± 0.07 b（< 1%）	17.91 ± 0.10 a（2%）	17.47 ± 0.20 b（1%）
	β-阿朴胡萝卜素醛β-apo-8'-carotenal	16.09 ± 0.04 a（< 1%）	13.47 ± 0.12 c（< 1%）	14.95 ± 0.18 b（< 1%）
	β-隐黄质β- cryptoxanthin	19.22 ± 0.04 a（< 1%）	16.52 ± 0.14 b（1%）	18.76 ± 0.48 a（1%）
	总量 Total	27.32 ± 0.05 a	23.78 ± 0.08 c	25.26 ± 0.11 b

Identification of Pigment Components and Key Genes in Carotenoid Pathway in Mutants of Chimeric Ananas comosus var. bracteatus

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