Anatomical Observation on the Graft Union Between Potato and Wolfberry

doi:10.16420/j.issn.0513-353x.2021-0198

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 868-874.doi: 10.16420/j.issn.0513-353x.2021-0198

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Anatomical Observation on the Graft Union Between Potato and Wolfberry

QI Lipan¹^,², LI Yue², WANG Lei², FENG Yan², WANG Kuan², YIN Jiang², GUO Huachun¹^,^**()

1. Tuber and Root Crop Research Institute,Yunnan Agricultural University,Kunming 650201,China
2. Rain-fed Agricultrue Research Center,Hebei North University,Zhangjiakou,Hebei 075000,China

Received:2021-09-28 Revised:2022-01-18 Online:2022-04-25 Published:2022-04-24
Contact: GUO Huachun E-mail:ynghc@126.com

Abstract

Abstract:

In order to reveal the graft union process between potato and wolfberry,anatomical observation of potato/wolfberry grafting system was carried out using paraffin section technique. The results showed that the potato/wolfberry grafting system grew vigorously and could complete the entire reproductive process. The union process of potato/wolfberry grafting could be divided into four stages:(1) isolation layer generation period,5 d after grafting,an isolation layer was formed at the grafting interface;(2) callus formation period,9 d after grafting,the isolation layer cells dedifferentiated,finally callus was formed;(3) callus differentiation stage,13 d after grafting,the callus differentiated,forming callus bridge;(4) new vascular tissue formation period,17 d after grafting,new phloem,xylem and vascular bundle bridges were formed which made the rootstock and scion fully combined,and the union process was finished.

Key words: potato, wolfberry, paraffin section, grafting union

CLC Number:

S532

QI Lipan, LI Yue, WANG Lei, FENG Yan, WANG Kuan, YIN Jiang, GUO Huachun. Anatomical Observation on the Graft Union Between Potato and Wolfberry[J]. Acta Horticulturae Sinica, 2022, 49(4): 868-874.

Figures/Tables 7

Fig. 1 Sampling diagram

Fig. 2 Observation of growth morphology of potato after grafting with wolfberry 1:Grafting for 1 d;2:The leaves of grafted plants became larger;3:Grafted plants union;4:Scion grow aerial potatoes;5:Budding stem in the scion base;6:Scion internodes became shorter;7:Scion flowering;8:Enlarged fertilized ovary.

Fig. 3 Microstructure of wolfberry stem and potato stem Ep:epidermis;Pd:pericyte;Ph:phloem;Xy:xylem;Co:Collenchyma;Pi:pith;Si T:Sieve tube;Ph P:phloem parenchyma cells;Pi R:Pith rays;V:Vessels.

Fig. 4 Microstructure at the grafting interface after 5d of grafting St:Rootstock;Sc:Scion;IL:Isolation layer.

Fig. 5 Microstructure at the grafting interface after grafting for 9 d Xy:Xylem;Ci:Callus;Ph:Phloem;St:Rootstock;Sc:Scion.

Fig. 6 Microstructure at the grafting interface after grafting for 13 d Xy:Xylem;Ci:Callus;Ph:Phloem;Ca:Cambium;St:Rootstock;Sc:Scion.

Fig. 7 Microstructure at grafting interface after grafting for 17 d Scx:Scion xylem;Vb:Vascular bundle bridge;Stp:Rootstock phloem;Xy:Xylem;Ph:Phloem;Ca:Cambium;Pi:Pith.

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Anatomical Observation on the Graft Union Between Potato and Wolfberry

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