Effects of Citrus Yellow Vein Clearing Virus on Photosynthetic Characteristics and Chloroplast Ultrastructure of Lemon

doi:10.16420/j.issn.0513-353x.2021-0391

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 861-867.doi: 10.16420/j.issn.0513-353x.2021-0391

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Effects of Citrus Yellow Vein Clearing Virus on Photosynthetic Characteristics and Chloroplast Ultrastructure of Lemon

WANG Ying¹, QIN Yangyang¹, ZENG Ting¹, LIAO Ping¹, ZHANG Wei², ZHOU Yan¹^,^*(), ZHOU Changyong¹^,^*()

1. National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China
2. Plant Protection Station,Department of Agriculture and Rural Affairs of Sichuan Province,Chengdu 610041,China

Received:2021-06-15 Revised:2021-09-07 Online:2022-04-25 Published:2022-04-24
Contact: ZHOU Yan,ZHOU Changyong E-mail:zhouyan@cric.cn;zhoucy@cric.cn

Abstract

Abstract:

Three-year-old Eureka lemon seedlings citrus yellow vein clearing virus（CYVCV）infection were used as test materials. After the seedlings showed typical symptoms such as yellowing and vein clearing,the following parameters as chlorophyll content,chlorophyll fluorescence,photosynthetic parameters and enzyme activities of lemon leaves etc.,were measured or monitored,respectively. The results showed that chlorophyll content,net photosynthetic rate（P_n）,stomatal conductance（G_s）,potential photochemical efficiency（F_v/F_o）and maximum photochemical efficiency（F_v/F_m）in CYVCV infected lemon had been significantly reduced,whereas the initial fluorescence（F_o）,catalase activity（CAT）and malondialdehyde（MDA）content had been significantly increased. There was no significant changes in the activities of peroxidase（POD）and superoxide（SOD）dismutase. After infection it was found that,the chloroplasts swelled and deformed due to the increase of starch grains,the stroma and grana lamellas were loose by using transmission electron microscope（TEM）,and a large number of osmophilic granules were observed. The results indicated that CYVCV infection destroyed the chloroplast structure and reduced the photosynthesis in lemon leaf.

Key words: Citrus, Citrus yellow vein clearing virus, lemon, photosynthesis, chloroplast ultrastructure

CLC Number:

S666.1

WANG Ying, QIN Yangyang, ZENG Ting, LIAO Ping, ZHANG Wei, ZHOU Yan, ZHOU Changyong. Effects of Citrus Yellow Vein Clearing Virus on Photosynthetic Characteristics and Chloroplast Ultrastructure of Lemon[J]. Acta Horticulturae Sinica, 2022, 49(4): 861-867.

Figures/Tables 4

References 36

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处理 Treatment	Chl.a/ （mg · g^-1）	Chl. b/ （mg · g^-1）	Chl. a+b/ （mg · g^-1）	Chl. a/b	P_n/ （mol · m^-2· s^-1）	G_s/ (mol · m^-2 · s^-1)	C_i/ （mol · mol^-1）
对照Control	1.558 ± 0.066	0.839 ± 0.066	2.380 ± 0.118	1.864 ± 0.074	7.833 ± 0.462	86.33 ± 9.074	264.3 ± 18.50
病株 Infected plant	0.239 ± 0.014^**	0.181 ± 0.007^**	0.421 ± 0.021^**	1.316 ± 0.030^**	5.200 ± 0.781^*	58.33 ± 5.508^*	282.0 ± 17.35

处理 Treatment	Chl.a/ （mg · g^-1）	Chl. b/ （mg · g^-1）	Chl. a+b/ （mg · g^-1）	Chl. a/b	P_n/ （mol · m^-2· s^-1）	G_s/ (mol · m^-2 · s^-1)	C_i/ （mol · mol^-1）
对照Control	1.558 ± 0.066	0.839 ± 0.066	2.380 ± 0.118	1.864 ± 0.074	7.833 ± 0.462	86.33 ± 9.074	264.3 ± 18.50
病株 Infected plant	0.239 ± 0.014^**	0.181 ± 0.007^**	0.421 ± 0.021^**	1.316 ± 0.030^**	5.200 ± 0.781^*	58.33 ± 5.508^*	282.0 ± 17.35

处理 Treatment	初始荧光 F_o	潜在光化学效率 F_v/F_o	最大光化学效率 F_v/F_m	光淬灭系数 q_P	非光淬灭系数 q_N
对照 Control	447.8 ± 18.36	3.061 ± 0.326a	0.7470 ± 0.019	1.006 ± 0.014	0.027 ± 0.005
病株 Infected plant	586.8 ± 12.35 a^*	2.090 ± 0.127^*	0.6536 ± 0.012^*	0.907 ± 0.024^**	0.013 ± 0.001^*

处理 Treatment	初始荧光 F_o	潜在光化学效率 F_v/F_o	最大光化学效率 F_v/F_m	光淬灭系数 q_P	非光淬灭系数 q_N
对照 Control	447.8 ± 18.36	3.061 ± 0.326a	0.7470 ± 0.019	1.006 ± 0.014	0.027 ± 0.005
病株 Infected plant	586.8 ± 12.35 a^*	2.090 ± 0.127^*	0.6536 ± 0.012^*	0.907 ± 0.024^**	0.013 ± 0.001^*

处理 Treatment	CAT/（nmol · g^-1 FW）	SOD/（U · g^-1 FW）	POD/（U · g^-1 FW）	MDA/（nmol · g^-1 FW）
健康对照Control	842.9 ± 54.99	132.8 ± 1.083	309.1 ± 22.68	28.82 ± 0.106
感病Infected plant	1 580.0 ± 180.2^*	184.6 ± 17.35	297.2 ± 6.291	103.9 ± 8.377^**

Effects of Citrus Yellow Vein Clearing Virus on Photosynthetic Characteristics and Chloroplast Ultrastructure of Lemon

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