The Heterologous Expression of Oncidium OnGI Promotes Flowering in Arabidopsis thaliana

doi:10.16420/j.issn.0513-353x.2021-0182

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 841-850.doi: 10.16420/j.issn.0513-353x.2021-0182

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The Heterologous Expression of Oncidium OnGI Promotes Flowering in Arabidopsis thaliana

FANG Nengyan, FAN Ronghui, LUO Yuanhua, KONG Lan, LIN Rongyan, YE Xiuxian, LIN Bing, ZHONG Huaiqin^*(), HUANG Minling^*()

Fujian Engineering Research Center of Characteristic Floriculture,Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China

Received:2021-11-01 Revised:2022-03-22 Online:2022-04-25 Published:2022-04-24
Contact: ZHONG Huaiqin,HUANG Minling E-mail:zhqeast@163.com;huangml618@163.com

Abstract

Abstract:

In this study,it was found that long-day condition could promote the flower bud differentiation of Oncidium under different photoperiod treatments. The OnGI gene of Oncidium was cloned,and its coding region was 3 483 bp long,encoding 1 161 amino acids. The results of multiple sequence alignment showed that it had the highest homology with Dendrobium nobile DnGI（86.57%）. The phylogenetic tree analysis showed that it was classified into the same group with other orchids. Tissue expression analysis showed that the expression of OnGI was the highest in flowers,especially at the initial stage of bud formation,and in leaves at different growth stages,the expression of OnGI peaked at the stage of flower bud differentiation. Under different photoperiod conditions,the expression pattern of OnGI was different,similar to that of Arabidopsis thaliana. The results of subcellular localization showed that OnGI was localized in the nucleus. Overexpression of OnGI in Arabidopsis thaliana could lead to earlier flowering. Based on the above results,we speculate that there may be a similar photoperiod regulation mechanism of GI expression in Arabidopsis thaliana and to regulate plant flowering.

Key words: Oncidium, OnGI, photoperiod, expression, flowering

CLC Number:

S682.31

FANG Nengyan, FAN Ronghui, LUO Yuanhua, KONG Lan, LIN Rongyan, YE Xiuxian, LIN Bing, ZHONG Huaiqin, HUANG Minling. The Heterologous Expression of Oncidium OnGI Promotes Flowering in Arabidopsis thaliana[J]. Acta Horticulturae Sinica, 2022, 49(4): 841-850.

Figures/Tables 8

Table 1 Sequences of primers

用途 Application	引物名称 Primer name	引物序列（5′-3′） Sequence
克隆 Clone	OnGI-F	ATGTCTGCATCGTGTCAGAAGTG
	OnGI-R	CTAGCTGGGAATACGAAAACCCAA
转基因植株验证 Validation of transgenic plants	pEarleyGate104-Ver-F	CAGATCTCGAGCTCAAGCTTCGAAT
转基因植株验证 Validation of transgenic plants	pEarleyGate104-Ver-R	ACAACGTGCACAACAGAATTGAAAGC
qPCR	OnGI1-qF	GCAAGAGATGATGAGCAAGG
	OnGI1-qR	TAAGTGTCTCAGGGCTTCC
	OnActin-qF	AATGTGCCTGCTATGTATGTTGCT
	OnActin-qR	GGGCATATCCTTCGTAGATTGT
半定量PCR	RT-OnGI-F	AGCAAGGTCAAAGAATGTGGAGGAT
Semi-quantitative RT-PCR	RT-OnGI-R	TGTCTGAATGCCAGTTGGTGATGTA
	RT-AtGI-F	CTGTCTTTCTCCGTTGTTTCACTGT
	RT-AtGI-R	TCATTCCGTTCTTCTCTGTTGTTGG
	RT-AtTUB-F	CTCAAGAGGTTCTCAGCAGTA
	RT-AtTUB-R	TCACCTTCTTCATCCGCAGTT

Fig. 1 Effect of photoperiod on bud formation rate of Oncidium

Fig. 2 Agarose electrophoresis of OnGI amplification products

Fig. 3 Phylogenetic analysis of the GI proteins from different plant species Percentage represents protein site coverage.

Fig. 4 Expression analysis of OnGI A. Different tissues;B. In leaves at different developmental stages;C. In flowers at different developmental stages. Different lowercase letters represent significant difference（P < 0.05）.

Fig. 5 Expression analysis of OnGI in leaves under different photoperiods

Fig. 6 Subcellular localization of OnGI

Fig. 7 The expression of OnGI in transgenic Arabidopsis thaliana plants（A）and the phenotype（B,C） WT:Wild type;OE-1-OE-3:OnGI overexpression of transgenic Arabidopsis thaliana lines.

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The Heterologous Expression of Oncidium OnGI Promotes Flowering in Arabidopsis thaliana

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