Studies on Transcriptome During Fruiting Body Development of Lentinula edodes

doi:10.16420/j.issn.0513-353x.2021-0660

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 801-815.doi: 10.16420/j.issn.0513-353x.2021-0660

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Studies on Transcriptome During Fruiting Body Development of Lentinula edodes

SHEN Nan, ZHANG Jingcheng, WANG Chengchen, BIAN Yinbing, XIAO Yang^*()

Institute of Applied Mycology,Huazhong Agricultural University,Wuhan 430070,China

Received:2021-08-26 Revised:2022-03-21 Online:2022-04-25 Published:2022-04-24
Contact: XIAO Yang E-mail:xiaoyang@mail.hzau.edu.cn

Abstract

Abstract:

Samples from different stages and tissues（pileus and stipe of fruiting body）during the development of Lentinula edodes were collected and sequenced. By transcriptomic analyses,we identified differentially expressed genes. Then,gene expression trend,gene co-expression network and differentially alternative splicing event were analyzed. Results showed that a large number of CAZyme genes were up-regulated in mycelia browning. Genes associated with signal transduction,such as photoreceptors,and genes related to melanin biosynthesis may also be involved in mycelia browning. Primordium formation may be associated with genes involved in environmental factor sensing,ribosome biogenesis,fungal cell wall remodeling,protein degradation and chromatin modification. Genes associated with DNA replication and protein degradation may play important roles in pileus development,while genes encoding glycoside hydrolases may function in pileus opening. Genes encoding carbonic anhydrase,chitinases,GH55 and cytochrome P450s may serve the functions in stipe elongation.

Key words: Lentinula edodes, fruiting body development, transcriptome, spatiotemporal expression, co-expression network, alternative splicing

CLC Number:

S646.12

SHEN Nan, ZHANG Jingcheng, WANG Chengchen, BIAN Yinbing, XIAO Yang. Studies on Transcriptome During Fruiting Body Development of Lentinula edodes[J]. Acta Horticulturae Sinica, 2022, 49(4): 801-815.

Figures/Tables 7

References 65

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样品	功能 Function	基因编号	基因功能注释
Sample	功能 Function	Gene ID	Gene annotation
MY vs. PR	碳水化合物代谢相关 Related to carbohydrate metabolism	jgi.p\|Lenedo1\|1049584	糖苷水解酶家族10蛋白
			Glycoside hydrolase family 10 protein
		jgi.p\|Lenedo1\|1114896	内切葡聚糖酶V类似蛋白
			Endoglucanase V-like protein
		jgi.p\|Lenedo1\|1038064	糖苷水解酶家族61蛋白
			Glycoside hydrolase family 61 protein
		jgi.p\|Lenedo1\|1159040	糖苷水解酶家族95蛋白
			Glycoside hydrolase family 95 protein
		jgi.p\|Lenedo1\|1158159	糖苷水解酶家族10蛋白
			Glycoside hydrolase family 10 protein
		jgi.p\|Lenedo1\|464359	糖苷水解酶 Glycoside hydrolase
		jgi.p\|Lenedo1\|1073151	糖苷水解酶家族18蛋白
			Glycoside hydrolase family 18 protein
	环境因子感知及信号转导相关 Related to environmental factor perception and signaling	jgi.p\|Lenedo1\|1035471	Rheb小单体GTPase Rheb small monomeric gtpase
		jgi.p\|Lenedo1\|1083501	COP9信号小体复合亚基4
			COP9 signalosome complex subunit 4
		jgi.p\|Lenedo1\|557311	假定水通道蛋白4 Putative aquaporin 4
	细胞壁相关 Related to cell wall	jgi.p\|Lenedo1\|1053705	扩展蛋白 Expansin
	蛋白合成与降解相关 Related to protein synthesis and degradation	jgi.p\|Lenedo1\|1100937	酸性蛋白酶 Acid protease
		jgi.p\|Lenedo1\|1060434	40S核糖体蛋白 S7 40S ribosomal protein S7
		jgi.p\|Lenedo1\|607412	核糖体蛋白L4 Ribosomal protein L4
		jgi.p\|Lenedo1\|1042368	三肽基肽酶A Tripeptidyl peptidase A
		jgi.p\|Lenedo1\|1039990	26S蛋白酶体亚基 P45 26S proteasome subunit P45
		jgi.p\|Lenedo1\|1092580	泛素结合酶E2 Ubiquitin-conjugating enzyme E2
		jgi.p\|Lenedo1\|81445	肽酶s41家族蛋白 Peptidase s41 family protein
		jgi.p\|Lenedo1\|1033416	核糖体蛋白L28e Ribosomal protein L28e
	其他功能 Other functions	jgi.p\|Lenedo1\|1059083	细胞色素P450 Cytochrome P450
		jgi.p\|Lenedo1\|1066578	细胞色素P450 Cytochrome p450
PR vs. D1ST		jgi.p\|Lenedo1\|717494	MFS转运蛋白 MFS general substrate transporter

样品	功能 Function	基因编号	基因功能注释
Sample	功能 Function	Gene ID	Gene annotation
MY vs. PR	碳水化合物代谢相关 Related to carbohydrate metabolism	jgi.p\|Lenedo1\|1049584	糖苷水解酶家族10蛋白
			Glycoside hydrolase family 10 protein
		jgi.p\|Lenedo1\|1114896	内切葡聚糖酶V类似蛋白
			Endoglucanase V-like protein
		jgi.p\|Lenedo1\|1038064	糖苷水解酶家族61蛋白
			Glycoside hydrolase family 61 protein
		jgi.p\|Lenedo1\|1159040	糖苷水解酶家族95蛋白
			Glycoside hydrolase family 95 protein
		jgi.p\|Lenedo1\|1158159	糖苷水解酶家族10蛋白
			Glycoside hydrolase family 10 protein
		jgi.p\|Lenedo1\|464359	糖苷水解酶 Glycoside hydrolase
		jgi.p\|Lenedo1\|1073151	糖苷水解酶家族18蛋白
			Glycoside hydrolase family 18 protein
	环境因子感知及信号转导相关 Related to environmental factor perception and signaling	jgi.p\|Lenedo1\|1035471	Rheb小单体GTPase Rheb small monomeric gtpase
		jgi.p\|Lenedo1\|1083501	COP9信号小体复合亚基4
			COP9 signalosome complex subunit 4
		jgi.p\|Lenedo1\|557311	假定水通道蛋白4 Putative aquaporin 4
	细胞壁相关 Related to cell wall	jgi.p\|Lenedo1\|1053705	扩展蛋白 Expansin
	蛋白合成与降解相关 Related to protein synthesis and degradation	jgi.p\|Lenedo1\|1100937	酸性蛋白酶 Acid protease
		jgi.p\|Lenedo1\|1060434	40S核糖体蛋白 S7 40S ribosomal protein S7
		jgi.p\|Lenedo1\|607412	核糖体蛋白L4 Ribosomal protein L4
		jgi.p\|Lenedo1\|1042368	三肽基肽酶A Tripeptidyl peptidase A
		jgi.p\|Lenedo1\|1039990	26S蛋白酶体亚基 P45 26S proteasome subunit P45
		jgi.p\|Lenedo1\|1092580	泛素结合酶E2 Ubiquitin-conjugating enzyme E2
		jgi.p\|Lenedo1\|81445	肽酶s41家族蛋白 Peptidase s41 family protein
		jgi.p\|Lenedo1\|1033416	核糖体蛋白L28e Ribosomal protein L28e
	其他功能 Other functions	jgi.p\|Lenedo1\|1059083	细胞色素P450 Cytochrome P450
		jgi.p\|Lenedo1\|1066578	细胞色素P450 Cytochrome p450
PR vs. D1ST		jgi.p\|Lenedo1\|717494	MFS转运蛋白 MFS general substrate transporter

Studies on Transcriptome During Fruiting Body Development of Lentinula edodes

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