Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

doi:10.16420/j.issn.0513-353x.2021-0202

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 758-768.doi: 10.16420/j.issn.0513-353x.2021-0202

• Research Papers • Previous Articles Next Articles

Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

ZHAO Jianrong¹, YANG Yuan¹^,², QIN Gaihua²^,^*(), LIU Chunyan², YU Qing²^,³, JIA Botao², SU Ying²^,³, CAO Zhen², LI Jiyu²^,^*()

1. College of Resource and Environment,Anhui Science and Technology University,Fengyang,Anhui 233100,China
2. Key Laboratory of Horticultural Crop Genetic Improvement and Eco-physiology of Anhui Province,Key Laboratory of Fruit Quality and Developmental Biology,Institute of Horticulture Research,Anhui Academy of Agricultural Sciences,Hefei 230001,China
3. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China

Received:2021-11-23 Revised:2022-01-12 Online:2022-04-25 Published:2022-04-24
Contact: QIN Gaihua,LI Jiyu E-mail:qghahstu@163.com;lijiyugx@163.com

Abstract

Abstract:

In order to fully understand the mechanism of K⁺ uptake,bioinformatics analysis and function study of related genes of HAK/KUP/KT family in pomegranate were carried out in this study. Eighteen of HAK/KUP/KT family genes each encoded 689-837 amino acids were identified from the pomegranate genome. The promoter region contains several regulatory elements related to stress,and the gene-coding proteins contain a K⁺ transport domain（GVVYGDLGTSPLY）. Phylogenetic analysis showed that the HAK/KUP/KT family genes of pomegranate,Arabidopsis thaliana,grape and pear could be divided into four clusters（Ⅰ-Ⅳ）. RNA-seq analysis showed that most genes of cluster Ⅱ and cluster Ⅲ were expressed in different tissues of pomegranate,while PgrHAK5 in clusterⅠwas only specifically expressed in roots. qRT-PCR analysis showed that PgrHAK5 was highly expressed in roots,and its expression was induced by potassium deficiency stress. Subcellular localization indicated that the protein encoded by PgrHAK5 was localized on the plasma membrane,and PgrHAK5 can complement the defect of the yeast K⁺ transport mutant CY162,which suggest that PgrHAK5 play an important role in K⁺ transport in pomegranate roots.

Key words: pomegranate, potassium transporters, PgrHAK5, functional

CLC Number:

S665.4

ZHAO Jianrong, YANG Yuan, QIN Gaihua, LIU Chunyan, YU Qing, JIA Botao, SU Ying, CAO Zhen, LI Jiyu. Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate[J]. Acta Horticulturae Sinica, 2022, 49(4): 758-768.

Figures/Tables 6

References 43

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名称 Name	基因号 ID	染色体号 Chr.	氨基酸 aa	蛋白质分子量/kD MW	等电点 pI	不稳定系数II Coefficient of instability	跨膜区 Transmem- brane domain	亚细胞定位 Subcellular localization
PgrHAK1	Pgr022478.1	Chr02	788	88.31	7.58	40.47	13	液泡膜 Vacuolar
PgrHAK2	Pgr025731.1	Chr09	798	88.95	7.87	40.61	13	液泡膜 Vacuolar
PgrHAK3	Pgr028419.1	Chr03	786	87.46	8.84	44.52	12	液泡膜 Vacuolar
PgrHAK4	Pgr017987.1	Chr05	745	83.65	9.15	40.44	12	质膜 Plasma membrane
PgrHAK5	Pgr009913.1	scaffold21	810	90.23	8.63	34.14	12	质膜 Plasma membrane
PgrHAK6	Pgr024378.1	Chr01	783	87.91	8.49	41.77	12	液泡膜 Vacuolar
PgrHAK7	Pgr016451.1	Chr06	757	84.37	5.12	41.43	10	质膜 Plasma membrane
PgrHAK8	Pgr014342.1	Chr07	779	87.06	8.00	37.49	10	液泡膜 Vacuolar
PgrHAK9	Pgr008140.1	Chr09	808	90.09	5.66	41.94	12	液泡膜 Vacuolar
PgrHAK10	Pgr027044.1	Chr03	794	89.00	7.31	42.90	13	液泡膜 Vacuolar
PgrHAK11	Pgr029120.1	Chr07	800	89.40	8.62	35.17	13	质膜 Plasma membrane
PgrHAK12	Pgr009693.1	scaffold21	837	92.45	5.46	43.09	12	液泡膜 Vacuolar
PgrHAK13	Pgr009692.3	scaffold21	697	78.48	8.56	41.39	10	质膜 Plasma membrane
PgrHAK14	Pgr018572.1	Chr08	772	87.36	6.55	36.03	11	液泡膜 Vacuolar
PgrHAK15	Pgr023110.1	Chr03	689	77.41	8.75	32.10	12	质膜 Plasma membrane
PgrHAK16	Pgr011385.2	Chr05	757	84.26	8.86	34.05	12	质膜 Plasma membrane
PgrHAK17	Pgr011384.1	Chr05	799	89.40	8.92	38.17	12	质膜 Plasma membrane
PgrHAK18	Pgr026837.2	Chr01	695	77.11	8.12	41.49	12	质膜 Plasma membrane

名称 Name	基因号 ID	染色体号 Chr.	氨基酸 aa	蛋白质分子量/kD MW	等电点 pI	不稳定系数II Coefficient of instability	跨膜区 Transmem- brane domain	亚细胞定位 Subcellular localization
PgrHAK1	Pgr022478.1	Chr02	788	88.31	7.58	40.47	13	液泡膜 Vacuolar
PgrHAK2	Pgr025731.1	Chr09	798	88.95	7.87	40.61	13	液泡膜 Vacuolar
PgrHAK3	Pgr028419.1	Chr03	786	87.46	8.84	44.52	12	液泡膜 Vacuolar
PgrHAK4	Pgr017987.1	Chr05	745	83.65	9.15	40.44	12	质膜 Plasma membrane
PgrHAK5	Pgr009913.1	scaffold21	810	90.23	8.63	34.14	12	质膜 Plasma membrane
PgrHAK6	Pgr024378.1	Chr01	783	87.91	8.49	41.77	12	液泡膜 Vacuolar
PgrHAK7	Pgr016451.1	Chr06	757	84.37	5.12	41.43	10	质膜 Plasma membrane
PgrHAK8	Pgr014342.1	Chr07	779	87.06	8.00	37.49	10	液泡膜 Vacuolar
PgrHAK9	Pgr008140.1	Chr09	808	90.09	5.66	41.94	12	液泡膜 Vacuolar
PgrHAK10	Pgr027044.1	Chr03	794	89.00	7.31	42.90	13	液泡膜 Vacuolar
PgrHAK11	Pgr029120.1	Chr07	800	89.40	8.62	35.17	13	质膜 Plasma membrane
PgrHAK12	Pgr009693.1	scaffold21	837	92.45	5.46	43.09	12	液泡膜 Vacuolar
PgrHAK13	Pgr009692.3	scaffold21	697	78.48	8.56	41.39	10	质膜 Plasma membrane
PgrHAK14	Pgr018572.1	Chr08	772	87.36	6.55	36.03	11	液泡膜 Vacuolar
PgrHAK15	Pgr023110.1	Chr03	689	77.41	8.75	32.10	12	质膜 Plasma membrane
PgrHAK16	Pgr011385.2	Chr05	757	84.26	8.86	34.05	12	质膜 Plasma membrane
PgrHAK17	Pgr011384.1	Chr05	799	89.40	8.92	38.17	12	质膜 Plasma membrane
PgrHAK18	Pgr026837.2	Chr01	695	77.11	8.12	41.49	12	质膜 Plasma membrane

Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

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