High Temperature Treatment Generates Unreduced Pollen in Paeonia suffruticosa

doi:10.16420/j.issn.0513-353x.2020-0815

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 581-589.doi: 10.16420/j.issn.0513-353x.2020-0815

• Research Papers • Previous Articles Next Articles

High Temperature Treatment Generates Unreduced Pollen in Paeonia suffruticosa

CHENG Shiping^*(), YAO Pengqiang, GENG Xining, LIU Chunyang, XIE Lihua

Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Woody Plant,School of Chemistry and Environmental Engineering,Pingdingshan University,Pingdingshan,Henan 467000,China

Received:2021-06-22 Revised:2022-01-24 Online:2022-03-25 Published:2022-03-25
Contact: CHENG Shiping E-mail:shipingcheng@163.com

Abstract

Abstract:

In this research,five-year Paeonia suffruticosa‘Fengdan’was chosen as an experimental material. On the basis of mastering meiosis process of pollen mother cells,“a non-in-vitro branch bud heat treatment device of trees”（ZL200610113448.X）was used to implement pollen chromosome doubling of P. suffruticosa under high-temperature processing,a suitable technique for high temperature-induced pollen chromosome doubling in P. suffruticosa was established. The findings indicated that meiosis process of pollen mother cells had correlation with the external form of flower buds and anther color changes. When flower buds remained between“small wind bell”stage to“big wind bell”stage,namely the diameter of flower buds was 13 mm,meiosis process of pollen mother cells reached diplonema—metaphaseⅠ. The treatment combination of 40 ℃ for 4 h produced the greatest amount of diploid pollen（up to 21.98% of the total yield）.

Key words: Paeonia suffruticosa, triploid breeding, meiosis process, 2n pollen, high temperature

CLC Number:

S685.11

CHENG Shiping, YAO Pengqiang, GENG Xining, LIU Chunyang, XIE Lihua. High Temperature Treatment Generates Unreduced Pollen in Paeonia suffruticosa[J]. Acta Horticulturae Sinica, 2022, 49(3): 581-589.

Figures/Tables 5

References 39

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温度/℃ Temperature	时间/h Duration	花粉总数 Total number of pollen	2n花粉总数 The number of 2n pollen	花粉直径/μm Diameter of pollen		2n花粉诱导率/% 2n pollen yield
温度/℃ Temperature	时间/h Duration	花粉总数 Total number of pollen	2n花粉总数 The number of 2n pollen	单倍型花粉 Haploid pollen	2n花粉 2n pollen	2n花粉诱导率/% 2n pollen yield
38	2	421	22	40.32 ± 0.24	56.50 ± 2.64	5.19 ± 0.58
	4	408	29	40.44 ± 0.64	57.16 ± 4.46	7.06 ± 0.77
	6	412	28	38.58 ± 0.36	58.32 ± 5.72	6.77 ± 0.64
40	2	406	45	38.72 ± 0.46	58.70 ± 2.64	11.17 ± 0.87
	4	414	91	40.60 ± 0.32	59.04 ± 3.18	21.98 ± 3.65
	6	403	67	37.56 ± 0.44	58.50 ± 2.54	16.58 ± 0.98
42	2	421	43	38.46 ± 1.80	59.16 ± 2.50	10.16 ± 0.95
	4	413	51	40.36 ± 1.50	59.52 ± 4.66	12.29 ± 0.67
	6	418	48	40.66 ± 1.23	59.76 ± 5.12	11.32 ± 1.74
44	2	423	32	36.22 ± 1.12	58.32 ± 2.70	7.50 ± 1.13
	4	416	41	39.26 ± 0.56	59.16 ± 4.42	9.86 ± 0.41
	6	409	36	40.24 ± 1.10	59.70 ± 2.64	8.97 ± 2.43
46	2	421	21	40.44 ± 0.70	58.24 ± 4.42	4.92 ± 0.68
	4	432	26	38.64 ± 0.50	59.76 ± 3.12	6.04 ± 0.36
	6	412	18	41.10 ± 1.36	59.56 ± 4.10	4.35 ± 0.40
对照Control	—	409	0	39.44 ± 0.82	—	0

温度/℃ Temperature	时间/h Duration	花粉总数 Total number of pollen	2n花粉总数 The number of 2n pollen	花粉直径/μm Diameter of pollen		2n花粉诱导率/% 2n pollen yield
温度/℃ Temperature	时间/h Duration	花粉总数 Total number of pollen	2n花粉总数 The number of 2n pollen	单倍型花粉 Haploid pollen	2n花粉 2n pollen	2n花粉诱导率/% 2n pollen yield
38	2	421	22	40.32 ± 0.24	56.50 ± 2.64	5.19 ± 0.58
	4	408	29	40.44 ± 0.64	57.16 ± 4.46	7.06 ± 0.77
	6	412	28	38.58 ± 0.36	58.32 ± 5.72	6.77 ± 0.64
40	2	406	45	38.72 ± 0.46	58.70 ± 2.64	11.17 ± 0.87
	4	414	91	40.60 ± 0.32	59.04 ± 3.18	21.98 ± 3.65
	6	403	67	37.56 ± 0.44	58.50 ± 2.54	16.58 ± 0.98
42	2	421	43	38.46 ± 1.80	59.16 ± 2.50	10.16 ± 0.95
	4	413	51	40.36 ± 1.50	59.52 ± 4.66	12.29 ± 0.67
	6	418	48	40.66 ± 1.23	59.76 ± 5.12	11.32 ± 1.74
44	2	423	32	36.22 ± 1.12	58.32 ± 2.70	7.50 ± 1.13
	4	416	41	39.26 ± 0.56	59.16 ± 4.42	9.86 ± 0.41
	6	409	36	40.24 ± 1.10	59.70 ± 2.64	8.97 ± 2.43
46	2	421	21	40.44 ± 0.70	58.24 ± 4.42	4.92 ± 0.68
	4	432	26	38.64 ± 0.50	59.76 ± 3.12	6.04 ± 0.36
	6	412	18	41.10 ± 1.36	59.56 ± 4.10	4.35 ± 0.40
对照Control	—	409	0	39.44 ± 0.82	—	0

类型Type	萌发率/% Germination rate	花粉管长度/μm Pollen tube length
单倍型花粉 Haploid pollen	70.94 ± 3.74	115.64 ± 36.94
2n花粉 2n pollen	82.27 ± 5.22	133.54 ± 30.02

类型Type	萌发率/% Germination rate	花粉管长度/μm Pollen tube length
单倍型花粉 Haploid pollen	70.94 ± 3.74	115.64 ± 36.94
2n花粉 2n pollen	82.27 ± 5.22	133.54 ± 30.02

High Temperature Treatment Generates Unreduced Pollen in Paeonia suffruticosa

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