Identification of TGA Gene Family in Peach and Analysis of Expression Mode Involved in a BABA-Induced Disease Resistance

doi:10.16420/j.issn.0513-353x.2020-1000

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 265-280.doi: 10.16420/j.issn.0513-353x.2020-1000

• Research Papers • Previous Articles Next Articles

Identification of TGA Gene Family in Peach and Analysis of Expression Mode Involved in a BABA-Induced Disease Resistance

LI Chunhong¹^,², WANG Kaituo¹^,²^,^*(), LEI Changyi¹, XU Feng³, JI Nana², JIANG Yongbo¹

¹College of Life Science and Engineering,Chongqing Three Gorges University,Chongqing 404000,China
²College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China
³College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo,Zhejiang 315211,China

Received:2021-10-30 Revised:2022-01-18 Online:2022-02-25 Published:2022-02-28
Contact: WANG Kaituo E-mail:wangkaituo83@gmail.com

Abstract

Abstract:

The highly conserved amino acid sequences of BRLZ（PF07716）and DOG1（PF14144）domains were used to identify the TGA family members,and the results showed that 15 members of the TGA family from the peach genome could be identified through bioinformatics analysis. The TGAs were unevenly distributed on the 1st,2rd,6th and 7th Prunus persica chromosomes. The protein parameters of the determined TGA TFs listed as follows：the sizes ranging from 333 to 546 aa,molecular weights ranging from 37.07 to 61.47 kD,the isoelectric points ranging from 6.01 to 8.59,respectively. And all members of the TGA family were located in the nucleus. According to the phylogenetic relationship, the TGA family of Arabidopsis thaliana,Glycine max,Solanum lycopersicum,Oryza sativa and Prunus persica was divided into five subfamilies,among which the peach TGA family members were mainly distributed inⅠ,Ⅱ and Ⅳ clades. Moreover,the promoter regions of the TGA members contained at least one plant hormone or stress response-related elements. In addition,the RNA-sequencing data exhibited that the expressions of the 15 TGA family members were potentiated by BABA treatment and/or Rhizopus stolonifer infection,and PpTGA1-1 expression was rapidly up-regulated within 12 h after stimulation. Further investigation indicated that the physical interaction between PpTGA1-1 and PpNPR1 provided the DNA binding capacity required by PpNPR1 for its activation of pathogenesis-related genes（PR）and consequently induced the priming resistance in harvested peaches. Collectively,a distinct member of TGA gene family in Prunus persica,particularly in PpTGA1-1,directly modified the PpNPR1 and thus exerted a crucial regulatory function for defensive response.

Key words: Prunus persica, TGA, transcription factor, β-aminobutyric acid, induced resistance, priming, Rhizopus rot

CLC Number:

S662.1

LI Chunhong, WANG Kaituo, LEI Changyi, XU Feng, JI Nana, JIANG Yongbo. Identification of TGA Gene Family in Peach and Analysis of Expression Mode Involved in a BABA-Induced Disease Resistance[J]. Acta Horticulturae Sinica, 2022, 49(2): 265-280.

Figures/Tables 13

References 44

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所用载体 Vector	引物名称 Primer name	寡核苷酸引物对 Oligonucleotide primers
pGADT7	PpNPR1-SmaI-F PpNPR1-BamHI-R	GGCCAGTGAATTCCACCCGGGCCCGGGATGGAATTCAAAGCCGGAGTC CAGCTCGAGCTCGATGGATCCGGATCCTTGATTGAGGGTGAGCATTCCA
pGBKT7	PpTGA1-1-SmaI-F PpTGA1-1-BamHI-R	CATGGAGGCCGAATTCCCGGGATGAATTCTCCATCCACCCAGTT ATGCGGCCGCTGCAGGTCGACGGCAGGCTCACGAGGACG

所用载体 Vector	引物名称 Primer name	寡核苷酸引物对 Oligonucleotide primers
pGADT7	PpNPR1-SmaI-F PpNPR1-BamHI-R	GGCCAGTGAATTCCACCCGGGCCCGGGATGGAATTCAAAGCCGGAGTC CAGCTCGAGCTCGATGGATCCGGATCCTTGATTGAGGGTGAGCATTCCA
pGBKT7	PpTGA1-1-SmaI-F PpTGA1-1-BamHI-R	CATGGAGGCCGAATTCCCGGGATGAATTCTCCATCCACCCAGTT ATGCGGCCGCTGCAGGTCGACGGCAGGCTCACGAGGACG

基因名称 Gene name	蛋白ID（NCBI） Protein ID	编码序列长度/bp CDS length	蛋白特性 Protein property
基因名称 Gene name	蛋白ID（NCBI） Protein ID	编码序列长度/bp CDS length	氨基酸数 Number of amino acids	分子量/kD MW	等电点 pI	不稳定系数 Instability index	平均亲水性 GRAVY
PpTGA1-1 PpTGA1-2 PpTGA2.2 PpTGA7-1 PpTGA7-2 PpTGA7-3 PpTGA9-1 PpTGA9-2 PpTGA9-3 PpTGA10-1 PpTGA10-2 PpTGA10-3 PpHBP-1b-1 PpHBP-1b-2 PpHBP-1b-3	XP_007205406.1 XP_020421616.1 XP_007218367.2 XP_020411363.1 XP_020411364.1 XP_007222089.2 XP_007205044.2 XP_020422804.1 XP_020422805.1 XP_007204568.1 XP_020423301.1 XP_020423302.1 XP_020423805.1 XP_007226716.1 XP_020413849.1	1 092 1 089 1 002 1 131 1 116 1 104 1 596 1 515 1 467 1 641 1 638 1 641 1 359 1 395 1 221	363 362 333 376 371 367 531 504 488 546 545 546 452 464 406	40.99 40.86 37.07 42.78 42.12 41.67 58.59 55.55 53.87 61.47 61.39 61.47 49.95 51.67 45.14	6.41 6.41 8.59 6.51 6.51 6.37 6.45 6.58 6.32 6.64 6.64 6.64 7.84 6.01 6.38	49.13 48.70 58.49 52.03 51.20 50.29 56.81 56.70 56.54 67.80 67.75 67.80 62.22 40.31 41.33	-0.468 -0.459 -0.570 -0.594 -0.587 -0.584 -0.538 -0.555 -0.520 -0.757 -0.757 -0.757 -0.616 -0.539 -0.509

基因名称 Gene name	蛋白ID（NCBI） Protein ID	编码序列长度/bp CDS length	蛋白特性 Protein property
基因名称 Gene name	蛋白ID（NCBI） Protein ID	编码序列长度/bp CDS length	氨基酸数 Number of amino acids	分子量/kD MW	等电点 pI	不稳定系数 Instability index	平均亲水性 GRAVY
PpTGA1-1 PpTGA1-2 PpTGA2.2 PpTGA7-1 PpTGA7-2 PpTGA7-3 PpTGA9-1 PpTGA9-2 PpTGA9-3 PpTGA10-1 PpTGA10-2 PpTGA10-3 PpHBP-1b-1 PpHBP-1b-2 PpHBP-1b-3	XP_007205406.1 XP_020421616.1 XP_007218367.2 XP_020411363.1 XP_020411364.1 XP_007222089.2 XP_007205044.2 XP_020422804.1 XP_020422805.1 XP_007204568.1 XP_020423301.1 XP_020423302.1 XP_020423805.1 XP_007226716.1 XP_020413849.1	1 092 1 089 1 002 1 131 1 116 1 104 1 596 1 515 1 467 1 641 1 638 1 641 1 359 1 395 1 221	363 362 333 376 371 367 531 504 488 546 545 546 452 464 406	40.99 40.86 37.07 42.78 42.12 41.67 58.59 55.55 53.87 61.47 61.39 61.47 49.95 51.67 45.14	6.41 6.41 8.59 6.51 6.51 6.37 6.45 6.58 6.32 6.64 6.64 6.64 7.84 6.01 6.38	49.13 48.70 58.49 52.03 51.20 50.29 56.81 56.70 56.54 67.80 67.75 67.80 62.22 40.31 41.33	-0.468 -0.459 -0.570 -0.594 -0.587 -0.584 -0.538 -0.555 -0.520 -0.757 -0.757 -0.757 -0.616 -0.539 -0.509

Identification of TGA Gene Family in Peach and Analysis of Expression Mode Involved in a BABA-Induced Disease Resistance

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