Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (1): 187-199.doi: 10.16420/j.issn.0513-353x.2020-0781
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SONG Yun, JIA Mengjun, CAO Yaping, LI Zheng, HE Jiaxin, WANG Yongfei, ZHANG Xinrui, QIAO Yonggang()
Received:
2021-04-28
Revised:
2021-09-09
Online:
2022-01-25
Published:
2022-01-24
Contact:
QIAO Yonggang
E-mail:sxndqyg@126.com
CLC Number:
SONG Yun, JIA Mengjun, CAO Yaping, LI Zheng, HE Jiaxin, WANG Yongfei, ZHANG Xinrui, QIAO Yonggang. Analysis on Chloroplast Genomic Characteristics of Forsythia suspensa[J]. Acta Horticulturae Sinica, 2022, 49(1): 187-199.
类型 Type | 大小/bp Size | 基因数量 Number of genes | GC/% | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
LSC | SSC | IR | 总数 Total | Protein | tRNA | rRNA | 总计 Total | LSC | SSC | IR | 平均 Mean | |
长花柱 Long-styled morph | 87 142 | 17 810 | 25 717 | 156 386 | 80 | 30 | 4 | 114 | 35.8 | 31.8 | 43.2 | 37.8 |
短花柱 Short style type | 87 595 | 17 781 | 25 717 | 156 810 | 80 | 30 | 4 | 114 | 35.8 | 31.9 | 43.2 | 37.8 |
Table 1 Statistics on the chloroplast gene annotation of heterostyly Forsythia suspense
类型 Type | 大小/bp Size | 基因数量 Number of genes | GC/% | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
LSC | SSC | IR | 总数 Total | Protein | tRNA | rRNA | 总计 Total | LSC | SSC | IR | 平均 Mean | |
长花柱 Long-styled morph | 87 142 | 17 810 | 25 717 | 156 386 | 80 | 30 | 4 | 114 | 35.8 | 31.8 | 43.2 | 37.8 |
短花柱 Short style type | 87 595 | 17 781 | 25 717 | 156 810 | 80 | 30 | 4 | 114 | 35.8 | 31.9 | 43.2 | 37.8 |
功能 Function | 基因家族 Family Name | 基因ID Gene ID |
---|---|---|
光合作用相关基因(45) Photosynthesis related genes | 核酮糖-1,5-二磷酸羧化酶 Rubisco | rbcL |
ATP合酶 ATP Synthase | atpA,atpB,atpE,atpF*,atpH,atpI | |
NADH脱氢酶 NADH Dehydrogenase | ndhA*,ndhB*,ndhC,ndhD,ndhE,ndhF,ndhG,ndhH,ndhI,ndhJ,ndhK | |
细胞色素 Cytochrome | petA,petB*,petD*,petG,petL,petN,ccsA | |
光合系统Ⅰ PhotosystemⅠ | psaA,psaB,psaC,psaI,psaJ | |
光合系统Ⅱ PhotosystemⅡ | psbA,psbB,psbC,psbD,psbE,psbF,psbH,psbI,psbJ,psbK,psbL,psbM,psbN,psbT,psbZ | |
转录翻译相关基因(60) Transcription and translation related genes | 大亚基核糖体 Large Subunit of Ribosome | rpl2*,rpl14,rpl16*,rpl20,rpl22,rpl23,rpl32,rpl33,rpl36 |
小亚基核糖体Small Subunit of Ribosome | rps2,rps3,rps4,rps7,rps8,rps11,rps12*,rps14,rps15,rps16*,rps18,rps19 | |
DNA依赖性RNA聚合酶 DNA Dependent RNA Polymerase | rpoA,rpoB,rpoC1*,rpoC2 | |
核糖体RNA rRNA Genes | rrn5,rrn4.5,rrn16,rrn23 | |
转运RNA tRNA Genes | trnA-UGC*,trnC-GCA,trnD-GUC,trnE-UUC,trnF-GAA,trnfM-CAU,trnG-GCC*,trnG-UCC,trnH-GUG,trnI-CAU,trnI-GAU*,trnK-UUU*,trnL-CAA,trnL-UAA*,trnL-UAG,trnM-CAU,trnN-GUU,trnP-UGG,trnQ-UUG,trnR-ACG,trnR-UCU,trnS-GCU,trnS-GGA,trnS-UGA,trnT-GGU,trnT-UGU,trnV-GAC,trnV-UAC*,trnW-CCA,trnY-GUA | |
翻译起始因子 Translation Initiation Factor | infA | |
其他基因(4) Other genes | 乙酰辅酶A羧化酶 Acety1-CoA carboxylase | accD |
包膜蛋白 Envelop membrane protein | cemA | |
成熟酶 Maturase | matK | |
clpP样蛋白酶 clpP-like protease | clpP* | |
功能未知基因(5) Unknown | 保守开放阅读框Conserved open reading frames | ycf1,ycf2,ycf3*,ycf4,ycf15 |
Table 2 Functional classification of heterostyly Forsythia suspensa chloroplast genes
功能 Function | 基因家族 Family Name | 基因ID Gene ID |
---|---|---|
光合作用相关基因(45) Photosynthesis related genes | 核酮糖-1,5-二磷酸羧化酶 Rubisco | rbcL |
ATP合酶 ATP Synthase | atpA,atpB,atpE,atpF*,atpH,atpI | |
NADH脱氢酶 NADH Dehydrogenase | ndhA*,ndhB*,ndhC,ndhD,ndhE,ndhF,ndhG,ndhH,ndhI,ndhJ,ndhK | |
细胞色素 Cytochrome | petA,petB*,petD*,petG,petL,petN,ccsA | |
光合系统Ⅰ PhotosystemⅠ | psaA,psaB,psaC,psaI,psaJ | |
光合系统Ⅱ PhotosystemⅡ | psbA,psbB,psbC,psbD,psbE,psbF,psbH,psbI,psbJ,psbK,psbL,psbM,psbN,psbT,psbZ | |
转录翻译相关基因(60) Transcription and translation related genes | 大亚基核糖体 Large Subunit of Ribosome | rpl2*,rpl14,rpl16*,rpl20,rpl22,rpl23,rpl32,rpl33,rpl36 |
小亚基核糖体Small Subunit of Ribosome | rps2,rps3,rps4,rps7,rps8,rps11,rps12*,rps14,rps15,rps16*,rps18,rps19 | |
DNA依赖性RNA聚合酶 DNA Dependent RNA Polymerase | rpoA,rpoB,rpoC1*,rpoC2 | |
核糖体RNA rRNA Genes | rrn5,rrn4.5,rrn16,rrn23 | |
转运RNA tRNA Genes | trnA-UGC*,trnC-GCA,trnD-GUC,trnE-UUC,trnF-GAA,trnfM-CAU,trnG-GCC*,trnG-UCC,trnH-GUG,trnI-CAU,trnI-GAU*,trnK-UUU*,trnL-CAA,trnL-UAA*,trnL-UAG,trnM-CAU,trnN-GUU,trnP-UGG,trnQ-UUG,trnR-ACG,trnR-UCU,trnS-GCU,trnS-GGA,trnS-UGA,trnT-GGU,trnT-UGU,trnV-GAC,trnV-UAC*,trnW-CCA,trnY-GUA | |
翻译起始因子 Translation Initiation Factor | infA | |
其他基因(4) Other genes | 乙酰辅酶A羧化酶 Acety1-CoA carboxylase | accD |
包膜蛋白 Envelop membrane protein | cemA | |
成熟酶 Maturase | matK | |
clpP样蛋白酶 clpP-like protease | clpP* | |
功能未知基因(5) Unknown | 保守开放阅读框Conserved open reading frames | ycf1,ycf2,ycf3*,ycf4,ycf15 |
差异 Difference | 长花柱/bp Long style type site | 短花柱/bp Short style type site |
---|---|---|
SNP1 | - | 4 705(A) |
SNP2 | 6 923(T) | - |
SNP3 | - | 6 973(T) |
SNP4 | 21 955(G) | 21 956(A) |
SNP5 | 21 964(A) | 21 964(C) |
SNP6 | - | 31 777(C) |
Gap1 | - | 31 805 ~ 32 018 |
SNP7 | 32 238(G) | 32 454(T) |
SNP8 | 32 243(G) | 32 459(T) |
Gap2 | - | 32 624 ~ 32 859 |
SNP9 | - | 60 082(C) |
SNP10 | 115 112(A) | - |
Gap3 | 115 316 ~ 115 343 | - |
SNP11 | 115 705(G) | 115 528(C) |
SNP12 | 115 919(A) | 116 343(G) |
Table 3 Sequence differences of chloroplast genome of heterostyly Forsythia suspensa
差异 Difference | 长花柱/bp Long style type site | 短花柱/bp Short style type site |
---|---|---|
SNP1 | - | 4 705(A) |
SNP2 | 6 923(T) | - |
SNP3 | - | 6 973(T) |
SNP4 | 21 955(G) | 21 956(A) |
SNP5 | 21 964(A) | 21 964(C) |
SNP6 | - | 31 777(C) |
Gap1 | - | 31 805 ~ 32 018 |
SNP7 | 32 238(G) | 32 454(T) |
SNP8 | 32 243(G) | 32 459(T) |
Gap2 | - | 32 624 ~ 32 859 |
SNP9 | - | 60 082(C) |
SNP10 | 115 112(A) | - |
Gap3 | 115 316 ~ 115 343 | - |
SNP11 | 115 705(G) | 115 528(C) |
SNP12 | 115 919(A) | 116 343(G) |
名称 Name | 引物序列(5′-3′) Primer sequences | 验证结果 Validation result | |
---|---|---|---|
SNP1 | F:TAGACGAAGTAATCAAACAAT | SNP1长短花柱均有A Both long and short styles at SNP1 locus have A | |
R:TATCTAGTGTCAATCCAAGAC | |||
SNP2,SNP3 | F:TTGGACAGTAAGGATCACATT | SNP2长短花柱均有T Both long and short styles at SNP2 locus have T | |
R:TATCCTATCCCTTTTATTCAC | SNP3长短花柱均无T Both long and short styles at SNP3 locus have no T | ||
SNP4,SNP5 | F:TGGCAGTTCCGTCTATCACTTTATT | SNP4长短花柱均为G Both long and short styles at SNP4 locus are G | |
R:TAGTCCTTTGTGGCTCCGGTGGCGA | SNP5长短花柱均为A Both long and short styles at SNP5 locus are A | ||
Gap1,SNP6 | F:TTATCCATACCGAATCCAAAC | Gap1不存在 Gap1 does not exist | |
R:AGAGTAAAAAACAGATCAATCAAAG | SNP6长短花柱均有C Both long and short styles at SNP6 locus have C | ||
Gap2,SNP7,SNP8 | F:AATTGAGGTTACACAAAACCGGAGC | Gap2不存在 Gap2 does not exist | |
R:TTCCGAAATACCCCATACGAAATAC | SNP7长短花柱均为G, SNP8长短花柱均为G Both long and short styles at SNP7 locus are G; Both long and short styles at SNP8 locus are G; | ||
SNP9 | F:CAAAATCTAAGACTAAATCCAAATCG | SNP9长短花柱均无C Both long and short styles at SNP9 locus have no C | |
R:CAGAATAAACATAGAAATGCAATCAC | |||
Gap3,SNP10, SNP11 | F:CTGGAAGTGGAACAAAAGGTA | 长短花柱均有Gap3序列 Both long and short styles have Gap3 sequence | |
R:TGTTCAAAATGAATCCGTGCT | SNP10长短花柱均有A, SNP11长短花柱均无该位碱基 Both long and short styles at SNP10 locus have A while SNP11 have no it | ||
SNP12 | F:GGAATAATCTGAATCGACTTG | SNP12长短花柱均为G Both long and short styles at SNP12 locus are G | |
R:CAGAAAGTTAGACAAAAGGGT | 长花柱115947位、短花柱116371位均缺少T No.115947 long style and No.116371 short style lack T |
Table 4 PCR verification result statistics
名称 Name | 引物序列(5′-3′) Primer sequences | 验证结果 Validation result | |
---|---|---|---|
SNP1 | F:TAGACGAAGTAATCAAACAAT | SNP1长短花柱均有A Both long and short styles at SNP1 locus have A | |
R:TATCTAGTGTCAATCCAAGAC | |||
SNP2,SNP3 | F:TTGGACAGTAAGGATCACATT | SNP2长短花柱均有T Both long and short styles at SNP2 locus have T | |
R:TATCCTATCCCTTTTATTCAC | SNP3长短花柱均无T Both long and short styles at SNP3 locus have no T | ||
SNP4,SNP5 | F:TGGCAGTTCCGTCTATCACTTTATT | SNP4长短花柱均为G Both long and short styles at SNP4 locus are G | |
R:TAGTCCTTTGTGGCTCCGGTGGCGA | SNP5长短花柱均为A Both long and short styles at SNP5 locus are A | ||
Gap1,SNP6 | F:TTATCCATACCGAATCCAAAC | Gap1不存在 Gap1 does not exist | |
R:AGAGTAAAAAACAGATCAATCAAAG | SNP6长短花柱均有C Both long and short styles at SNP6 locus have C | ||
Gap2,SNP7,SNP8 | F:AATTGAGGTTACACAAAACCGGAGC | Gap2不存在 Gap2 does not exist | |
R:TTCCGAAATACCCCATACGAAATAC | SNP7长短花柱均为G, SNP8长短花柱均为G Both long and short styles at SNP7 locus are G; Both long and short styles at SNP8 locus are G; | ||
SNP9 | F:CAAAATCTAAGACTAAATCCAAATCG | SNP9长短花柱均无C Both long and short styles at SNP9 locus have no C | |
R:CAGAATAAACATAGAAATGCAATCAC | |||
Gap3,SNP10, SNP11 | F:CTGGAAGTGGAACAAAAGGTA | 长短花柱均有Gap3序列 Both long and short styles have Gap3 sequence | |
R:TGTTCAAAATGAATCCGTGCT | SNP10长短花柱均有A, SNP11长短花柱均无该位碱基 Both long and short styles at SNP10 locus have A while SNP11 have no it | ||
SNP12 | F:GGAATAATCTGAATCGACTTG | SNP12长短花柱均为G Both long and short styles at SNP12 locus are G | |
R:CAGAAAGTTAGACAAAAGGGT | 长花柱115947位、短花柱116371位均缺少T No.115947 long style and No.116371 short style lack T |
SSR类型 SSR Type | SSR重复碱基 SSR Repeat Base | SSR起始位置 SSR Start | SSR区域 SSR Region | SSR位置 SSR Location |
---|---|---|---|---|
单核苷酸(37) Mono-nucleotide | (A)10 | 4 842 | LSC | IGS |
(T)12 | 9 030 | LSC | IGS | |
(T)10 | 11 138 | LSC | IGS | |
(A)13 | 12 717 | LSC | IGS | |
(T)13 | 14 085 | LSC | IGS | |
(A)10 | 15 717 | LSC | IGS | |
(A)10 | 16 489 | LSC | IGS | |
(T)11 | 19 653 | LSC | CDS | |
(T)12 | 20 191 | LSC | CDS | |
(A)21 | 29 932 | LSC | IGS | |
(A)10 | 31 831 | LSC | IGS | |
(A)10 | 31 968 | LSC | IGS | |
(A)17 | 33 139 | LSC | IGS | |
(T)10 | 33 817 | LSC | IGS | |
(T)13 | 34 464 | LSC | IGS | |
(T)12 | 37 624 | LSC | IGS | |
(A)10 | 38 003 | LSC | IGS | |
(A)15 | 44 538 | LSC | IGS | |
(A)16 | 46 593 | LSC | intron | |
(A)11 | 46 852 | LSC | IGS | |
(T)14 | 48 603 | LSC | IGS | |
(T)10 | 49 569 | LSC | IGS | |
(T)12 | 50 845 | LSC | IGS | |
(T)10 | 59 736 | LSC | IGS | |
(T)10 | 61 617 | LSC | IGS | |
(T)10 | 63 559 | LSC | IGS | |
(T)10 | 66 245 | LSC | IGS | |
(T)10 | 69 156 | LSC | IGS | |
(T)11 | 72 217 | LSC | IGS | |
(T)10 | 73 677 | LSC | intron | |
(A)11 | 73 841 | LSC | intron | |
(T)10 | 74 697 | LSC | intron | |
(T)12 | 81 215 | LSC | CDS | |
(T)13 | 83 627 | LSC | IGS | |
(A)10 | 85 352 | LSC | intron | |
(T)17 | 86 822 | LSC | IGS | |
(T)17 | 117 894 | SSC | IGS | |
二核苷酸(7) Di-nucleotide | (AT)5 | 4 622 | LSC | IGS |
(AT)5 | 6 532 | LSC | IGS | |
(AT)5 | 21 032 | LSC | CDS | |
(TA)6 | 48 745 | LSC | IGS | |
(TA)6 | 49 264 | LSC | IGS | |
(TA)5 | 69 573 | LSC | IGS | |
(TA)6 | 69 885 | LSC | IGS | |
三核苷酸(1)Tri-nucleotide | (CCT)4 | 69 325 | LSC | IGS |
四核苷酸(5) Tetra-nucleotide | (AAAT)4 | 9 260 | LSC | IGS |
(TCTT)3 | 31 167 | LSC | IGS | |
(TTTA)3 | 55 057 | LSC | IGS | |
(GAAA)3 | 63 543 | LSC | IGS | |
(AAAG)3 | 73 367 | LSC | intron | |
五核苷酸(1) Penta-nucleotide | (TCTAT)3 | 9 434 | LSC | IGS |
复合型 Compound | - | 17 431 | LSC | IGS |
- | 38 479 | LSC | IGS | |
- | 57 033 | LSC | CDS-IGS | |
- | 71 524 | LSC | IGS | |
- | 78 279 | LSC | intron |
Table 5 The chloroplast genome SSR loci distributionin in heterostyly Forsythia suspensa
SSR类型 SSR Type | SSR重复碱基 SSR Repeat Base | SSR起始位置 SSR Start | SSR区域 SSR Region | SSR位置 SSR Location |
---|---|---|---|---|
单核苷酸(37) Mono-nucleotide | (A)10 | 4 842 | LSC | IGS |
(T)12 | 9 030 | LSC | IGS | |
(T)10 | 11 138 | LSC | IGS | |
(A)13 | 12 717 | LSC | IGS | |
(T)13 | 14 085 | LSC | IGS | |
(A)10 | 15 717 | LSC | IGS | |
(A)10 | 16 489 | LSC | IGS | |
(T)11 | 19 653 | LSC | CDS | |
(T)12 | 20 191 | LSC | CDS | |
(A)21 | 29 932 | LSC | IGS | |
(A)10 | 31 831 | LSC | IGS | |
(A)10 | 31 968 | LSC | IGS | |
(A)17 | 33 139 | LSC | IGS | |
(T)10 | 33 817 | LSC | IGS | |
(T)13 | 34 464 | LSC | IGS | |
(T)12 | 37 624 | LSC | IGS | |
(A)10 | 38 003 | LSC | IGS | |
(A)15 | 44 538 | LSC | IGS | |
(A)16 | 46 593 | LSC | intron | |
(A)11 | 46 852 | LSC | IGS | |
(T)14 | 48 603 | LSC | IGS | |
(T)10 | 49 569 | LSC | IGS | |
(T)12 | 50 845 | LSC | IGS | |
(T)10 | 59 736 | LSC | IGS | |
(T)10 | 61 617 | LSC | IGS | |
(T)10 | 63 559 | LSC | IGS | |
(T)10 | 66 245 | LSC | IGS | |
(T)10 | 69 156 | LSC | IGS | |
(T)11 | 72 217 | LSC | IGS | |
(T)10 | 73 677 | LSC | intron | |
(A)11 | 73 841 | LSC | intron | |
(T)10 | 74 697 | LSC | intron | |
(T)12 | 81 215 | LSC | CDS | |
(T)13 | 83 627 | LSC | IGS | |
(A)10 | 85 352 | LSC | intron | |
(T)17 | 86 822 | LSC | IGS | |
(T)17 | 117 894 | SSC | IGS | |
二核苷酸(7) Di-nucleotide | (AT)5 | 4 622 | LSC | IGS |
(AT)5 | 6 532 | LSC | IGS | |
(AT)5 | 21 032 | LSC | CDS | |
(TA)6 | 48 745 | LSC | IGS | |
(TA)6 | 49 264 | LSC | IGS | |
(TA)5 | 69 573 | LSC | IGS | |
(TA)6 | 69 885 | LSC | IGS | |
三核苷酸(1)Tri-nucleotide | (CCT)4 | 69 325 | LSC | IGS |
四核苷酸(5) Tetra-nucleotide | (AAAT)4 | 9 260 | LSC | IGS |
(TCTT)3 | 31 167 | LSC | IGS | |
(TTTA)3 | 55 057 | LSC | IGS | |
(GAAA)3 | 63 543 | LSC | IGS | |
(AAAG)3 | 73 367 | LSC | intron | |
五核苷酸(1) Penta-nucleotide | (TCTAT)3 | 9 434 | LSC | IGS |
复合型 Compound | - | 17 431 | LSC | IGS |
- | 38 479 | LSC | IGS | |
- | 57 033 | LSC | CDS-IGS | |
- | 71 524 | LSC | IGS | |
- | 78 279 | LSC | intron |
氨基酸 Amino acid | 密码子 Codon | 数量 Numer | RSCU | 氨基酸 Amino acid | 密码子 Codon | 数量 Numer | RSCU |
---|---|---|---|---|---|---|---|
Phe | UUU | 2 262 | 1.18 | Ser | UCU | 1 233 | 1.52 |
UUC | 1 582 | 0.82 | UCC | 872 | 1.07 | ||
Leu | UUA | 947 | 1.17 | UCA | 966 | 1.19 | |
UUG | 1 044 | 1.29 | UCG | 622 | 0.77 | ||
CUU | 1 043 | 1.29 | AGU | 700 | 0.86 | ||
CUC | 651 | 0.80 | AGC | 479 | 0.59 | ||
CUA | 700 | 0.87 | Pro | CCU | 703 | 1.10 | |
CUG | 469 | 0.58 | CCC | 619 | 0.97 | ||
Ile | AUU | 1 814 | 1.24 | CCA | 827 | 1.30 | |
AUC | 1 173 | 0.80 | CCG | 403 | 0.63 | ||
AUA | 1 412 | 0.96 | Thr | ACU | 707 | 1.22 | |
Met | AUG | 788 | 1.00 | ACC | 606 | 1.05 | |
Val | GUU | 831 | 1.42 | ACA | 663 | 1.15 | |
GUC | 435 | 0.74 | ACG | 334 | 0.58 | ||
GUA | 711 | 1.22 | Ala | GCU | 513 | 1.32 | |
GUG | 359 | 0.61 | GCC | 347 | 0.89 | ||
Tyr | UAU | 1 357 | 1.33 | GCA | 436 | 1.12 | |
UAC | 676 | 0.67 | GCG | 256 | 0.66 | ||
TER | UAA | 1 095 | 1.11 | Cys | UGU | 686 | 1.21 |
UAG | 786 | 0.80 | UGC | 449 | 0.79 | ||
UGA | 1 084 | 1.10 | Arg | CGU | 377 | 0.66 | |
His | CAU | 970 | 1.42 | CGC | 222 | 0.39 | |
CAC | 392 | 0.58 | CGA | 597 | 1.05 | ||
Gln | CAA | 1 110 | 1.44 | CGG | 397 | 0.70 | |
CAG | 428 | 0.56 | AGA | 1 177 | 2.08 | ||
Asn | AAU | 1 694 | 1.38 | AGG | 632 | 1.11 | |
AAC | 765 | 0.62 | Gly | GGU | 612 | 1.03 | |
Lys | AAA | 2 114 | 1.34 | GGC | 346 | 0.58 | |
AAG | 1 041 | 0.66 | GGA | 885 | 1.49 | ||
Asp | GAU | 1 089 | 1.44 | GGG | 531 | 0.89 | |
GAC | 426 | 0.56 | Glu | GAA | 1 364 | 1.38 | |
Trp | UGG | 706 | 1.00 | GAG | 613 | 0.62 |
Table 6 Relative synonymous codon usage(RSCU)of chloroplast genome in Forsythia suspensa heterostyly
氨基酸 Amino acid | 密码子 Codon | 数量 Numer | RSCU | 氨基酸 Amino acid | 密码子 Codon | 数量 Numer | RSCU |
---|---|---|---|---|---|---|---|
Phe | UUU | 2 262 | 1.18 | Ser | UCU | 1 233 | 1.52 |
UUC | 1 582 | 0.82 | UCC | 872 | 1.07 | ||
Leu | UUA | 947 | 1.17 | UCA | 966 | 1.19 | |
UUG | 1 044 | 1.29 | UCG | 622 | 0.77 | ||
CUU | 1 043 | 1.29 | AGU | 700 | 0.86 | ||
CUC | 651 | 0.80 | AGC | 479 | 0.59 | ||
CUA | 700 | 0.87 | Pro | CCU | 703 | 1.10 | |
CUG | 469 | 0.58 | CCC | 619 | 0.97 | ||
Ile | AUU | 1 814 | 1.24 | CCA | 827 | 1.30 | |
AUC | 1 173 | 0.80 | CCG | 403 | 0.63 | ||
AUA | 1 412 | 0.96 | Thr | ACU | 707 | 1.22 | |
Met | AUG | 788 | 1.00 | ACC | 606 | 1.05 | |
Val | GUU | 831 | 1.42 | ACA | 663 | 1.15 | |
GUC | 435 | 0.74 | ACG | 334 | 0.58 | ||
GUA | 711 | 1.22 | Ala | GCU | 513 | 1.32 | |
GUG | 359 | 0.61 | GCC | 347 | 0.89 | ||
Tyr | UAU | 1 357 | 1.33 | GCA | 436 | 1.12 | |
UAC | 676 | 0.67 | GCG | 256 | 0.66 | ||
TER | UAA | 1 095 | 1.11 | Cys | UGU | 686 | 1.21 |
UAG | 786 | 0.80 | UGC | 449 | 0.79 | ||
UGA | 1 084 | 1.10 | Arg | CGU | 377 | 0.66 | |
His | CAU | 970 | 1.42 | CGC | 222 | 0.39 | |
CAC | 392 | 0.58 | CGA | 597 | 1.05 | ||
Gln | CAA | 1 110 | 1.44 | CGG | 397 | 0.70 | |
CAG | 428 | 0.56 | AGA | 1 177 | 2.08 | ||
Asn | AAU | 1 694 | 1.38 | AGG | 632 | 1.11 | |
AAC | 765 | 0.62 | Gly | GGU | 612 | 1.03 | |
Lys | AAA | 2 114 | 1.34 | GGC | 346 | 0.58 | |
AAG | 1 041 | 0.66 | GGA | 885 | 1.49 | ||
Asp | GAU | 1 089 | 1.44 | GGG | 531 | 0.89 | |
GAC | 426 | 0.56 | Glu | GAA | 1 364 | 1.38 | |
Trp | UGG | 706 | 1.00 | GAG | 613 | 0.62 |
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