Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (12): 2349-2359.doi: 10.16420/j.issn.0513-353x.2020-0866
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XIAO Xiao, DING Liang, CONG Yu(), XU Xuling, QIAO Huan, HE Silong, XU Wenjian, XU Tianshun
Received:
2021-05-14
Revised:
2021-09-17
Published:
2022-01-04
Contact:
CONG Yu
E-mail:congy@phagelux.com
CLC Number:
XIAO Xiao, DING Liang, CONG Yu, XU Xuling, QIAO Huan, HE Silong, XU Wenjian, XU Tianshun. Isolation and Identification of a Lytic Bacteriophage Infecting Xanthomonas axonopodis pv. citri[J]. Acta Horticulturae Sinica, 2021, 48(12): 2349-2359.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0866
采样时间 Sampling time | 地理来源 Geographical origin | 菌株编号 Strain No. | 采集部位 Sampling organ | 柑橘品种 Citrus variety | ||
---|---|---|---|---|---|---|
2017 | 10-24 | 广西Guangxi | 武鸣Wuming | Xac-1 | 叶片Leaf | 沃柑Orah |
Xac-2 | 果实Fruit | 沃柑Orah | ||||
10-26 | 崇左Chongzuo | Xac-3 | 叶片Leaf | 茂谷柑Murcott | ||
Xac-4 | 果实Fruit | 茂谷柑Murcott | ||||
10-29 | 鹿寨Luzhai | Xac-5 | 叶片Leaf | 马水橘Mashui orange | ||
Xac-6 | 果实Fruit | 马水橘Mashui orange | ||||
11-02 | 河池Hechi | Xac-7 | 叶片Leaf | 砂糖橘Shatangju | ||
Xac-8 | 果实Fruit | 砂糖橘Shatangju | ||||
2018 | 10-17 | 湖南Hunan | 永州Yongzhou | Xac-9 | 叶片Leaf | 椪柑Ponkan |
Xac-10 | 果实Fruit | 椪柑Ponkan | ||||
10-20 | 麻阳Mayang | Xac-11 | 叶片Leaf | 纽荷尔Newhall | ||
Xac-12 | 果实Fruit | 纽荷尔Newhall | ||||
10-23 | 靖州Jingzhou | Xac-13 | 叶片Leaf | 血橙Blood orange | ||
Xac-14 | 果实Fruit | 血橙Blood orange | ||||
10-28 | 浙江Zhejiang | 苍南Cangnan | Xac-15 | 叶片Leaf | 柚 Pomelo | |
Xac-16 | 果实Fruit | 柚 Pomelo | ||||
11-02 | 江西Jiangxi | 赣州Ganzhou | Xac-17 | 叶片Leaf | 脐橙Navel orange |
Table 1 Isolation statistics of Xac
采样时间 Sampling time | 地理来源 Geographical origin | 菌株编号 Strain No. | 采集部位 Sampling organ | 柑橘品种 Citrus variety | ||
---|---|---|---|---|---|---|
2017 | 10-24 | 广西Guangxi | 武鸣Wuming | Xac-1 | 叶片Leaf | 沃柑Orah |
Xac-2 | 果实Fruit | 沃柑Orah | ||||
10-26 | 崇左Chongzuo | Xac-3 | 叶片Leaf | 茂谷柑Murcott | ||
Xac-4 | 果实Fruit | 茂谷柑Murcott | ||||
10-29 | 鹿寨Luzhai | Xac-5 | 叶片Leaf | 马水橘Mashui orange | ||
Xac-6 | 果实Fruit | 马水橘Mashui orange | ||||
11-02 | 河池Hechi | Xac-7 | 叶片Leaf | 砂糖橘Shatangju | ||
Xac-8 | 果实Fruit | 砂糖橘Shatangju | ||||
2018 | 10-17 | 湖南Hunan | 永州Yongzhou | Xac-9 | 叶片Leaf | 椪柑Ponkan |
Xac-10 | 果实Fruit | 椪柑Ponkan | ||||
10-20 | 麻阳Mayang | Xac-11 | 叶片Leaf | 纽荷尔Newhall | ||
Xac-12 | 果实Fruit | 纽荷尔Newhall | ||||
10-23 | 靖州Jingzhou | Xac-13 | 叶片Leaf | 血橙Blood orange | ||
Xac-14 | 果实Fruit | 血橙Blood orange | ||||
10-28 | 浙江Zhejiang | 苍南Cangnan | Xac-15 | 叶片Leaf | 柚 Pomelo | |
Xac-16 | 果实Fruit | 柚 Pomelo | ||||
11-02 | 江西Jiangxi | 赣州Ganzhou | Xac-17 | 叶片Leaf | 脐橙Navel orange |
菌株名称 Strain name | 菌株编号 Strain No. | 裂解结果 Lysis result |
---|---|---|
地毯草黄单胞菌柑橘致病变种 Xanthomonas axonopodis pv. citri | Xac-1 | +++ |
Xac-2 | +++ | |
Xac-3 | +++ | |
Xac-4 | +++ | |
Xac-5 | +++ | |
Xac-6 | +++ | |
Xac-7 | +++ | |
Xac-8 | +++ | |
Xac-9 | +++ | |
Xac-10 | +++ | |
Xac-11 | +++ | |
Xac-12 | +++ | |
Xac-13 | +++ | |
Xac-14 | +++ | |
Xac-15 | +++ | |
Xac-16 | +++ | |
Xac-17 | +++ | |
大肠埃希菌Escherichia coli | GIM 1.573 | - |
枯草芽孢杆菌Bacillus subtilis | GIM 1.784 | - |
短小芽孢杆菌Bacillus pumilus | GIM 1.801 | - |
苏云金芽孢杆菌Bacillus thuringiensis | GIM 1.150 | - |
棕色固氮菌Azotobacter vinelandii | GIM 1.802 | - |
Table 2 Lysis ability of phage Xac-P9 to 22 bacteria
菌株名称 Strain name | 菌株编号 Strain No. | 裂解结果 Lysis result |
---|---|---|
地毯草黄单胞菌柑橘致病变种 Xanthomonas axonopodis pv. citri | Xac-1 | +++ |
Xac-2 | +++ | |
Xac-3 | +++ | |
Xac-4 | +++ | |
Xac-5 | +++ | |
Xac-6 | +++ | |
Xac-7 | +++ | |
Xac-8 | +++ | |
Xac-9 | +++ | |
Xac-10 | +++ | |
Xac-11 | +++ | |
Xac-12 | +++ | |
Xac-13 | +++ | |
Xac-14 | +++ | |
Xac-15 | +++ | |
Xac-16 | +++ | |
Xac-17 | +++ | |
大肠埃希菌Escherichia coli | GIM 1.573 | - |
枯草芽孢杆菌Bacillus subtilis | GIM 1.784 | - |
短小芽孢杆菌Bacillus pumilus | GIM 1.801 | - |
苏云金芽孢杆菌Bacillus thuringiensis | GIM 1.150 | - |
棕色固氮菌Azotobacter vinelandii | GIM 1.802 | - |
感染复数 MOI | Xac-P9 噬菌体数(PFU) Number of Xac-P9 phages | 宿主菌数(CFU) Number of bacteria | Xac-P9效价(PFU · mL-1) Titer of Xac-P9 |
---|---|---|---|
1 000 | 1.0 × 109 | 1.0 × 106 | 1.2 × 106 |
100 | 1.0 × 108 | 1.0 × 106 | 3.8 × 106 |
10 | 1.0 × 107 | 1.0 × 106 | 1.2 × 107 |
1 | 1.0 × 106 | 1.0 × 106 | 3.9 × 108 |
0.1 | 1.0 × 105 | 1.0 × 106 | 4.5 × 108 |
0.01 | 1.0 × 104 | 1.0 × 106 | 7.8 × 108 |
0.001 | 1.0 × 103 | 1.0 × 106 | 6.6 × 109 |
0.0001 | 1.0 × 102 | 1.0 × 106 | 4.1 × 109 |
Table 3 Optimal MOI of phage Xac-P9
感染复数 MOI | Xac-P9 噬菌体数(PFU) Number of Xac-P9 phages | 宿主菌数(CFU) Number of bacteria | Xac-P9效价(PFU · mL-1) Titer of Xac-P9 |
---|---|---|---|
1 000 | 1.0 × 109 | 1.0 × 106 | 1.2 × 106 |
100 | 1.0 × 108 | 1.0 × 106 | 3.8 × 106 |
10 | 1.0 × 107 | 1.0 × 106 | 1.2 × 107 |
1 | 1.0 × 106 | 1.0 × 106 | 3.9 × 108 |
0.1 | 1.0 × 105 | 1.0 × 106 | 4.5 × 108 |
0.01 | 1.0 × 104 | 1.0 × 106 | 7.8 × 108 |
0.001 | 1.0 × 103 | 1.0 × 106 | 6.6 × 109 |
0.0001 | 1.0 × 102 | 1.0 × 106 | 4.1 × 109 |
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