Studies on Flowering Characteristics and Breeding System of Hemerocallis citrina‘Datong Huanghua’

doi:10.16420/j.issn.0513-353x.2020-1024

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1541-1551.doi: 10.16420/j.issn.0513-353x.2020-1024

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Studies on Flowering Characteristics and Breeding System of Hemerocallis citrina‘Datong Huanghua’

LI Sen¹^,²^,³, LIU Juan¹^,²^,³, GONG Feifei¹^,²^,³, WANG Nan¹, DU Wei¹^,²^,³, ZHANG Lingling¹^,²^,³, KANG Xiuping¹^,²^,³, XING Guoming¹^,²^,³^,^*()

¹College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China
²Greenhouse Vegetable Quality Improving Synergy Innovation Center of Shanxi Province,Taigu,Shanxi 030801,China
³Datong Daylily Industrial Development Research Institute,Datong,Shanxi 037004,China

Received:2021-03-03 Revised:2021-07-26 Online:2021-08-25 Published:2021-09-06
Contact: XING Guoming E-mail:xingguoming@163.com

Abstract

Abstract:

The sexual propagation of Hemerocallis citrina‘Datong Huanghua’was determined by the flowering characteristics and breeding system. To promote the process of hybrid breeding,we investigated the flowing traits including the morphological characteristics of flowers,blooming process,pollen vitality changes under varied storage temperatures,stigma receptivity,breeding system types,and hybrid seed setting rate. The results showed that：（1）The flowering period of‘Datong Huanghua’started from mid-to-late June and ended at the end of July or early August. Flower buds at maturity show yellow-green color and turn bright yellow when opened;the florescence last about 12 hours. The pollen grains are oval with clear and deep net-like carvings on the outer wall.（2）With the extension of storage period,the pollen viability exhibited consistent decreasing trends at varied storage temperatures. During the 60-day storage period,the pollen viability was the highest under-80 ℃ storage condition among 25,4,-20 and-40 ℃.（3）After the petals opening,the stigma receptivity increased firstly and reached its maximum at 3 hours;then it started to decrease and lost the receptivity at 30 h.（4）The out crossing index（OCI）of‘Datong Huanghua’was 4 and the ratio of pollen and ovule（P/O）was 2 446.22. The fruit setting rate of natural cross-pollination was significantly higher than that of artificial self-pollination. Therefore,the breeding system of‘Datong Huanghua’is defined as facultative outcrossing type.（5）The multiple parental hybridization experiments revealed that the seed setting rate of‘Datong Huanghua’as male parent was higher than that of female parent.

Key words: Hemerocallis citrina, floral syndrome, stigma receptivity, beeding system, crossbreeding

CLC Number:

S644.3

LI Sen, LIU Juan, GONG Feifei, WANG Nan, DU Wei, ZHANG Lingling, KANG Xiuping, XING Guoming. Studies on Flowering Characteristics and Breeding System of Hemerocallis citrina‘Datong Huanghua’[J]. Acta Horticulturae Sinica, 2021, 48(8): 1541-1551.

Figures/Tables 8

References 29

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类群 Group	种质 Material	种源地 Origin	倍性 Ploidy
夜间开花 Nocturnal flowering	大同黄花 Datong Huanghua	中国山西大同 Datong,Shanxi,China	二倍体 Diplpoid
	六月花 Liuyuehua	中国湖南邵阳 Shaoyang,Hunan,China	二倍体 Diploid
	白花 Baihua	中国湖南邵阳 Shaoyang,Hunan,China	二倍体 Diploid
白天开花 Daytime flowering	蟠龙花 Panlonghua	中国浙江缙云 Jinyun,Zhejiang,China	二倍体 Diploid
	紫泉 Purple Waters	比利时 Belgium	二倍体 Diploid
	台东6号 Taidong 6	中国台湾 Taiwan,China	二倍体 Diploid
	海尔范 Frans Hals	荷兰 The Netherlands	二倍体 Diploid
	摇篮曲 Lullaby Baby	荷兰 The Netherlands	二倍体 Diploid
	历山野生 Lishan Yesheng	中国山西晋城Jincheng,Shanxi,China	三倍体 Triploid
	小石城 Rocket City	比利时Belgium	四倍体 Tetraploid

类群 Group	种质 Material	种源地 Origin	倍性 Ploidy
夜间开花 Nocturnal flowering	大同黄花 Datong Huanghua	中国山西大同 Datong,Shanxi,China	二倍体 Diplpoid
	六月花 Liuyuehua	中国湖南邵阳 Shaoyang,Hunan,China	二倍体 Diploid
	白花 Baihua	中国湖南邵阳 Shaoyang,Hunan,China	二倍体 Diploid
白天开花 Daytime flowering	蟠龙花 Panlonghua	中国浙江缙云 Jinyun,Zhejiang,China	二倍体 Diploid
	紫泉 Purple Waters	比利时 Belgium	二倍体 Diploid
	台东6号 Taidong 6	中国台湾 Taiwan,China	二倍体 Diploid
	海尔范 Frans Hals	荷兰 The Netherlands	二倍体 Diploid
	摇篮曲 Lullaby Baby	荷兰 The Netherlands	二倍体 Diploid
	历山野生 Lishan Yesheng	中国山西晋城Jincheng,Shanxi,China	三倍体 Triploid
	小石城 Rocket City	比利时Belgium	四倍体 Tetraploid

年份 Date	始花期/（M-D） Initial flowering stage	盛花期/（M-D） Blooming stage	末花期/（M-D） Last flowering stage	群体花期/d Flowering days
2018	06-27	07-05	08-05	40
2019	06-20	06-28	08-01	43
2020	06-15	06-25	07-30	46

年份 Date	始花期/（M-D） Initial flowering stage	盛花期/（M-D） Blooming stage	末花期/（M-D） Last flowering stage	群体花期/d Flowering days
2018	06-27	07-05	08-05	40
2019	06-20	06-28	08-01	43
2020	06-15	06-25	07-30	46

处理方式 Treatment	处理花朵数 Treatment number	坐果数 Fruit set number	坐果率/% Fruit set percentage
无融合生殖Apomixis	30	0	0
人工自花授粉Hand self-pollination	30	1	3.33
自然异花授粉Natural cross-pollination	30	12	40.00
自然授粉Open pollination	30	17	56.67

Studies on Flowering Characteristics and Breeding System of Hemerocallis citrina‘Datong Huanghua’

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Figures/Tables 8

References 29

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母本 Female parent	父本 Male parent	授粉花朵数 Number of pollina- tion	结实果数 Number of fruit set	结实率/% Fruit setting rate	单果种子数 Number of seeds/ Fruit	种子粒数 Number of seeds	发芽粒数 Number of germinated seeds	发芽率/% Germi- nation
大同黄花Datong Huanghua	海尔范 Frans Hals	400	37	9.25	5.75 ± 1.50	157	152	96.82
	台东6号 Taidong 6	700	64	9.14	8.09 ± 2.94	518	492	94.98
	摇篮曲 Lullaby Baby	850	51	6.00	6.78 ± 2.31	346	318	91.91
	白花 Bai Hua	500	26	5.20	3.62 ± 1.69	94	80	85.11
	六月花 Liuyue Hua	181	9	4.97	2.89 ± 1.37	26	26	100.00
	紫泉 Purple Waters	380	16	4.21	5.88 ± 2.15	94	87	92.55
	蟠龙花 Panlong Hua	720	30	4.17	3.50 ± 1.26	105	96	91.43
	历山野生 Lishan Yesheng	100	0	0	0	0	0	0
	小石城 Rocket City	50	0	0	0	0	0	0
六月花 Liuyue Hua	大同黄花Datong Huanghua	213	146	68.54	10.57 ± 3.80	1 543	1221	79.13
历山野生Lishan Yesheng	大同黄花Datong Huanghua	340	140	41.18	9.14 ± 2.97	1 280	1018	79.53
蟠龙花Panlong Hua	大同黄花Datong Huanghua	120	9	7.50	7.78 ± 2.53	70	56	80.00
小石城Rocket City	大同黄花Datong Huanghua	50	0	0	0	0	0	0

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