Changes of Fruit Abscission and Carbohydrate,ABA and Related Genes Expression in the Pericarp and Fruit Abscission Zone of Longan Under Starvation Stress

doi:10.16420/j.issn.0513-353x.2020-0538

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1457-1469.doi: 10.16420/j.issn.0513-353x.2020-0538

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Changes of Fruit Abscission and Carbohydrate,ABA and Related Genes Expression in the Pericarp and Fruit Abscission Zone of Longan Under Starvation Stress

YANG Weihai¹, ZENG Lizhen¹, XIAO Qiusheng¹, SHI Shengyou²^,^*()

¹College of Life Science and Resources and Environment,Yichun University,Yichun,Jiangxi 336000,China
²South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091,China

Received:2021-01-17 Revised:2021-04-06 Online:2021-08-25 Published:2021-09-06
Contact: SHI Shengyou E-mail:ssy7299@163.com

Abstract

Abstract:

In order to explore the regulation mechanism of longan fruit abscission induced by “starvation stress”,a treatment of girdling and defoliation was made on the bearing shoots of longan cultivar‘Chuliang’at the early stage of fruit development,simulating starvation stress induced by interrupting carbon supply to fruit cluster,and the levels of carbohydrate,abscisic acid（ABA）and related genes expression in the pericarp and the pedicel abscission zone were investigated at 0,1,3,5 and 6 d after treatment,respectively. The results showed that a severe fruit drop occurred at 5-6 d after starvation stress treatment,the contents of total soluble sugar and its components（glucose,fructose,sucrose）in pericarp were significantly decreased,and the reduction was relatively large during 3 d after treatment,with the decreases of sucrose and glucose significantly larger than fructose. Meanwhile,the similar changes in the levels of total soluble sugar and sucrose also occurred in abscission zone. After being treated,ABA content did not change obviously in pericarp,while increased significantly in abscission zone. Starvation stress generally decreased the expression of genes related to carbohydrate and ABA in the pericarp and abscission zone,the expressions of genes involved in sugar metabolism（NI,SS1,SPS1）,sugar signaling（HXK2,TPS1,SnRK2）and ABA metabolism（AAO3,NCED,BG）were significantly decreased during the early stage of fruit shedding,whereas NI,SS1,HXK2,SnRK2,AAO3 and NCED genes were expressed with up-regulation in the period of severe fruit drop,resulting in the sharply decreased sucrose,occurring of sugar signal and ABA accumulation. These results suggested that a crosstalk between sugar and ABA may be related to longan fruitlet abscission induced by carbohydrate starvation.

Key words: longan, girdling and defoliation, starvation stress, carbohydrate, abscisic acid, pericarp, abscission zone, fruit abscission

CLC Number:

S667.2

YANG Weihai, ZENG Lizhen, XIAO Qiusheng, SHI Shengyou. Changes of Fruit Abscission and Carbohydrate,ABA and Related Genes Expression in the Pericarp and Fruit Abscission Zone of Longan Under Starvation Stress[J]. Acta Horticulturae Sinica, 2021, 48(8): 1457-1469.

Figures/Tables 7

Table 1 The primer sequences used for qRT-PCR analysis

基因Gene	正向引物（5′-3′）Forward primer sequence	反向引物（5′-3′）Reverse primer sequence
Actin	TGCTATCCTTCGGTTGGACC	CGGACGATTTCCCGTTCAG
NI	GATTTTGAGGTTATGTCTCGCTG	GATTGTTCAGTGCTCGGATAAGA
SS1	AATTCCTTAATCGCCACCTCT	AGCATCATATTCTTGCCCTTG
SPS1	ATGGTGACAAAAATGAAGGTGG	TGCCTTTTGTGCTCCTCCA
HXK2	AGTGCTTTTGAAGATGGCTGAA	ATCCAACGGCTTGAAGACCT
TPS1	GGTCTTTGTGGGAGGCGTAT	CGGACTGTGAAGAAAGAACCC
SnRK2	GGCCAAAAGATTGATGAACATG	TTGCCTCGTCCTCGCTAAA
BG	AAAGGAACCCCAATGAGACC	ACCGTCATAGGTGAAAATCCC
AAO3	GACTGGGGTGCTTTACACTTTG	CGACTTCCATAGATAGCCTAGACG
NCED	ACTTGTGGAATGCTTGGGAG	CCTGATTTCGGACAAAACACTC

Fig. 1 Effect of girdling and defoliation treatment on fruitlet abscission of longan * indicated significant difference compared with the control（α = 0.05）. The same below.

Fig. 2 Changes in the contents of total soluble sugar and its composition in the longan pericarp after girdling and defoliation treatment

Fig. 3 Changes in the contents of total soluble sugar and its composition in the pedicel abscission zone of longan after girdling and defoliation treatment

Fig. 4 Changes of ABA content in the pericarp（A）and pedicel abscission zone（B）of longan after girdling and defoliation treatment

Fig. 5 Changes in the expression levels of genes involved in sugar metabolism and signaling and ABA metabolism in the pericarp of longan after girdling and defoliation treatment

Fig. 6 Changes in the expression levels of genes involved in sugar metabolism and signaling and ABA metabolism in the fruit abscission zone of longan after girdling and defoliation treatment

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Changes of Fruit Abscission and Carbohydrate,ABA and Related Genes Expression in the Pericarp and Fruit Abscission Zone of Longan Under Starvation Stress

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