QTL Mapping of Bolting and Flowering Traits Based on High Density Genetic Map of Radish

doi:10.16420/j.issn.0513-353x.2021-0201

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (7): 1273-1281.doi: 10.16420/j.issn.0513-353x.2021-0201

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QTL Mapping of Bolting and Flowering Traits Based on High Density Genetic Map of Radish

GAN Caixia¹^,^*(), CUI Lei¹, PANG Wenxing², WANG Aihua³, YU Xiaoqing¹, DENG Xiaohui¹^,^*(), SONG Liping³, PIAO Zhongyun²

¹Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement,Wuhan 430070,China
²College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China
³Wuhan Academy of Agricultural Sciences,Wuhan 430065,China

Received:2021-02-23 Revised:2021-05-26 Online:2021-07-25 Published:2021-08-10
Contact: GAN Caixia,DENG Xiaohui E-mail:gancaixiazhuanye@126.com;15927275107@163.com

Abstract

Abstract:

The late-bolting genes in radish were localized,and the candidate genes regulating flowering were explored. The main results are listed below：a high-density genetic linkage map was constructed and two QTL loci related to flowering and bolting were detected. Based on radish gene annotation and gene sequence alignment analysis,we detected five genes with the sequence variation between the parental lines,indicated those genes associated with bolting and flowering in radish. However,the detailed functions of the candidate gene during the radish bolting and flowering need further studies.

Key words: Raphanus sativus, bolting, flowering, QTL, candidate gene

CLC Number:

S631.1

GAN Caixia, CUI Lei, PANG Wenxing, WANG Aihua, YU Xiaoqing, DENG Xiaohui, SONG Liping, PIAO Zhongyun. QTL Mapping of Bolting and Flowering Traits Based on High Density Genetic Map of Radish[J]. Acta Horticulturae Sinica, 2021, 48(7): 1273-1281.

Figures/Tables 7

References 28

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播种年份/季节 Year/Season	世代 Generation	植株数量 Number of plants	抽薹时间/d Bolting time	开花时间/d Flowering time
2015/冬 2015/Winter	P₁	30	205	214
	P₂	30	138	155
	F₁	30	170	187
	F₂	129	180（150 ~ 193）	188（169 ~ 202）
2016/秋 2016/Autumn	P₁	30	207	220
	P₂	30	154	172
	F₁	30	167	184
	F_2︰3	97	171 ~ 195	185 ~ 207
2016/冬 2016/Winter	P₁	30	185	195
	P₂	30	140	147
	F₁	30	160	171
	F_2︰3	97	153 ~ 172	158 ~ 182

播种年份/季节 Year/Season	世代 Generation	植株数量 Number of plants	抽薹时间/d Bolting time	开花时间/d Flowering time
2015/冬 2015/Winter	P₁	30	205	214
	P₂	30	138	155
	F₁	30	170	187
	F₂	129	180（150 ~ 193）	188（169 ~ 202）
2016/秋 2016/Autumn	P₁	30	207	220
	P₂	30	154	172
	F₁	30	167	184
	F_2︰3	97	171 ~ 195	185 ~ 207
2016/冬 2016/Winter	P₁	30	185	195
	P₂	30	140	147
	F₁	30	160	171
	F_2︰3	97	153 ~ 172	158 ~ 182

连锁群 LG	QTL	季节 Season	置信区间/cM Confidence interval	标记区间 Marker interval	LOD		加性效应 Additive	显性效应 Dominance	R²/%
R01	qBT1	秋Autumn	3.61 ~ 11.51	R01_48351323 ~ R01_45976870	2.83		-0.20	-8.09	0.02
R09	qBT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	9.62		-12.04	11.67	0.60
R09	qBT2	冬Winter	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	13.37		-32.37	44.39	0.62
R01	qFT1	秋Autumn	6.67 ~ 8.52	R01_47574115 ~ R01_47530802	3.67	-0.07		-12.14	0.03
R09	qFT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	11.90	-18.05		12.08	0.86
		冬Winter			12.66	-38.79		41.38	0.59

连锁群 LG	QTL	季节 Season	置信区间/cM Confidence interval	标记区间 Marker interval	LOD		加性效应 Additive	显性效应 Dominance	R²/%
R01	qBT1	秋Autumn	3.61 ~ 11.51	R01_48351323 ~ R01_45976870	2.83		-0.20	-8.09	0.02
R09	qBT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	9.62		-12.04	11.67	0.60
R09	qBT2	冬Winter	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	13.37		-32.37	44.39	0.62
R01	qFT1	秋Autumn	6.67 ~ 8.52	R01_47574115 ~ R01_47530802	3.67	-0.07		-12.14	0.03
R09	qFT2	秋Autumn	28.49 ~ 59.65 28.49 ~ 59.65	R09_6700570 ~ R09_12999583 R09_6700570 ~ R09_12999583	11.90	-18.05		12.08	0.86
		冬Winter			12.66	-38.79		41.38	0.59

QTL Mapping of Bolting and Flowering Traits Based on High Density Genetic Map of Radish

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