Effects of Bjerkandera adusta‘M1’on Gray Mold Prevention and Growth Promotion in Tomato

doi:10.16420/j.issn.0513-353x.2020-0949

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 960-972.doi: 10.16420/j.issn.0513-353x.2020-0949

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Effects of Bjerkandera adusta‘M1’on Gray Mold Prevention and Growth Promotion in Tomato

ZHANG Jingjie, ZHU Yunyun, LU Yifan, LI Yong^*()

College of Resources and Environment,Southwest University,Chongqing 400715,China

Received:2020-11-30 Revised:2021-03-22 Online:2021-05-25 Published:2021-06-07
Contact: LI Yong E-mail:liyonmgwf@swu.edu.cn

Abstract

Abstract:

The biocontrol efficiency of a local isolate of Bjerkandera adusta‘M1’against the gray mold and growth promotion in tomato was studied in order to further understand its potential function in agriculture. The confrontation culture,fermentation broth inhibition and greenhouse pot experiments were conducted to evaluate the inhibitory effect ofBj. adusta‘M1’and its fermentation broth on theBotrytis cinerea Pers.,and their biocontrol efficiency against the tomato gray mold and plant growth promotion. The growth of Bo. cinerea was obviously suppressed by Bj. adusta‘M1’through the observations of their confrontation culture. Results from the optical and scanning electron microscopic observations showed that the hyphae ofBo. cinerea were interspersed and twined by Bj. adusta‘M1’,and resulted in hyphal swelling, atrophy and deformation,indicating a strong mycoparasitism. Results from the plate antagonism test indicated that the inhibition rate of theBj. adusta‘M1’fermentation broth againstBo. cinereareached up to 47.90%,which was equivalent to that of the chemical procymidone. Compared to the Bo. cinereaspore suspension application,the greenhouse pot experiment showed that the disease index of gray mold was significantly decrease,and 55.2% control effect was thus obtained;the activity of leaf peroxidase,superoxide dismutase and catalase was significantly increased by 29.57%,136.15% and 534.13%,respectively. Meanwhile,the malondialdehyde content and the relative electrolyte leakage rate were significantly reduced by 19.98% and 51.10%,indicating a reduction of the pathogen damage to plants. Plant height,stem thickness,aboveground biomass and leaf chlorophyll content in tomato plants were increased significantly by 63.92%,38.04%,87.08%,and 35.02%,respectively. These results demonstrate that Bj. adusta‘M1’,and its fermentation broth can inhibit theBo. cinerea growth and effectively control the gray mold,while promoting the tomato growth. This study concludes that Bj. adusta‘M1’has a great potential to control gray mold in the field.

Key words: tomato, Bjerkandera adusta, Botrytis cinerea, gray mold, plant growth

CLC Number:

S641.2

ZHANG Jingjie, ZHU Yunyun, LU Yifan, LI Yong. Effects of Bjerkandera adusta‘M1’on Gray Mold Prevention and Growth Promotion in Tomato[J]. Acta Horticulturae Sinica, 2021, 48(5): 960-972.

Figures/Tables 7

Fig. 1 Confront culture test of Bjerkandera adusta‘M1’andBotrytis cinerea A:Picture of the confrontation,the left side of the plate is Bj. adusta‘M1’,and the right side isBo. cinerea;B:Picture of control,which is only inoculated withBo. Cinerea.

Fig. 2 Antagonistic effect of Bjerkandera adusta‘M1’fermentation broth(BAFB)and Procymidone(Pr)onBotrytis cinerea in culture medium

Fig. 3 Effects of Bjerkandera adusta‘M1’on hyphal morphology ofBotrytis cinerea under optical microscope A:Normal hyphae of Bj. adusta‘M1’;B:Normal hyphae ofBo. cinerea;C-F:Abnormal hyphae of confrontation zone;Bj.:Hyphae of Bj. adusta‘M1’;Bo.:Hyphae of Bo. cinereaPers.;a:Swelling deformation;b:Winding;c:Penetration;d:Distortion and atrophy.

Fig. 4 The mycoparasitism of strain‘M1’againstBotrytis cinerea under scanning electron microscope Bj.:Hyphae of Bj. adusta‘M1’;Bo.:Hyphae of Bo. cinerea;a:Winding;b:Distortion and atrophy;c:Penetration.

Table 1 Control effect of sprayingBjerkandera adusta‘M1’fermentation broth(BAFB)and procymidone(Pr)on tomato plants inoculated withBotrytis cinerea(BCS)

处理 Treatment	病情指数 Disease index	防效/% Control effect
蒸馏水Sterile water	0 c	—
BCS	77.3 ± 4.99 a	—
BAFB + BCS	35.1 ± 6.00 b	55.2 ± 5.20 a
Pr + BCS	30.0 ± 6.48 b	61.4 ± 7.15 a

Fig. 5 Effects of spraying Bjerkandera adusta‘M1’fermentation broth(BAFB)and procymidone(Pr)on defense enzymes,malondialdehyde content and cell membrane permeability of tomato plants inoculated withBotrytis cinerea(BCS) Vertical bars represent mean ± SE value(n = 3),different lowercase letters in the same bar represent significant differences(P< 0.05)among different treatments under Duncan’s range test.

Fig. 6 Effect of spraying Bjerkandera adusta‘M1’fermentation broth(BAFB)and procymidone(Pr)on the growth of tomato plants inoculated withBotrytis cinerea(BCS) Vertical bars represent mean ±SE value(n= 3),different lowercase letters in the same bar represent significant differences(P< 0.05).among different treatments under Duncan’s range test.

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Effects of Bjerkandera adusta‘M1’on Gray Mold Prevention and Growth Promotion in Tomato

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