Genome-wide Identification and Expression Analysis of DRB Gene Family in Dimocarpus longan

doi:10.16420/j.issn.0513-353x.2020-0526

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 921-933.doi: 10.16420/j.issn.0513-353x.2020-0526

• Research Papers • Previous Articles Next Articles

Genome-wide Identification and Expression Analysis of DRB Gene Family in Dimocarpus longan

CAI Roudi, LI Xue, CHEN Yan, XU Xiaoping, CHEN Xiaohui, LAI Zhongxiong^*(), LIN Yuling^*()

Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Received:2020-12-23 Revised:2021-03-22 Online:2021-05-25 Published:2021-06-07
Contact: LAI Zhongxiong,LIN Yuling E-mail:Laizx01@163.com;buliang84@163.com

Abstract

Abstract:

In order to understand the potential functions of DRB(dsRNA-binding proteins)in longan,the bioinformatics analysis was carried out to identify members of the DRB gene family,and then their expression patterns were analyzed. The results showed that the DlDRB gene family contained eight members with two or three DSRM(double-stranded RNA binding motif)domains and could be distributed into five subgroups. The numbers of introns varied from one to seven. DlDRBs contain six kinds of motifs. The promoters ofDlDRBscontain a large number of light response elements,hormone response elements and stress response elements. DlDRBswere expressed in all stages of longan somatic embryogenesis and different organs. Under ABA,SA and MeJA treatments,the expression ofDlDRBsis clearly inhibited. This study indicated that members of the DlDRBs were highly conserved,they may be involved in the growth and development of somatic embryogenesis and organs in longan,and may be related to the response processes of ABA,SA and MeJA.

Key words: Dimocarpus longan, DRB gene family, genome-wide identification, promoter, bioinformatics, expression analysis

CLC Number:

S667.2

CAI Roudi, LI Xue, CHEN Yan, XU Xiaoping, CHEN Xiaohui, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of DRB Gene Family in Dimocarpus longan[J]. Acta Horticulturae Sinica, 2021, 48(5): 921-933.

Figures/Tables 8

References 39

[1]	Chang K Y, Ramos A. 2005. The double-stranded RNA-binding motif,a versatile macro-molecular docking platform. FEBS Journal, 272(9):2109-2117. doi: 10.1111/ejb.2005.272.issue-9 URL
[2]	Chen Xiaohui. 2018. Cloning and function analysis of DlDCLs in somatic embryogenesis of Dimocarpus longan Lour. [M. D. Dissertation]. Fuzhou:Fujian Agriculture and Forestry University. (in Chinese)
	陈晓慧. 2018. 龙眼体胚发生过程中DlDCLs的克隆与功能分析[硕士论文]. 福州:福建农林大学.
[3]	Chen Xiaohui, Bai Yu, Chen Xu, Li Hansheng, Lin Yuling, Lai Zhongxiong. 2017b. Molecular characterization and expression patterns of the DCL gene family during somatic embryogenesis in Dimocarpus longan Lour . Acta Botanica Boreali-Occidentalia Sinica, 37(11):120-2129. (in Chinese)
	陈晓慧, 白玉, 陈旭, 李汉生, 林玉玲, 赖钟雄. 2017b. 龙眼体胚发生过程中DCL基因的分子特性及表达分析. 西北植物学报, 37(11):2120-2129.
[4]	Chen Xiaohui, Bai Yu, Li Hansheng, Chen Xu, Liu Yuling, Lai Zhongxiong. 2017a. Promoter analysis and spatial and temporal expression of the DCL family gene in Dimocarpus longan Lour . Acta Botanica Boreali-Occidentalia Sinica, 37(10):1926-1933. (in Chinese)
	陈晓慧, 白玉, 李汉生, 陈旭, 林玉玲, 赖钟雄. 2017a. 龙眼DCL家族基因的启动子分析及时空表达. 西北植物学报, 37(10):1926-1933.
[5]	Clavel M, Pélissier T, Montavon T, Tschopp M A, Pouch-Pélissier M N, Descombin J, Jean V, Dunoyer P, Bousquet-Antonelli C, Deragon J M. 2016. Evolutionary history of double-stranded RNA binding proteins in plants;identification of new cofactors involved in easiRNA biogenesis. Plant Molecular Biology, 91(1-2):131-147. doi: 10.1007/s11103-016-0448-9 URL
[6]	Doornbos R F, Geraats B P, Kuramae E E, van Loon L C, Bakker P A. 2011. Effects of jasmonic acid,ethylene,and salicylic acid signaling on the rhizosphere bacterial community ofArabidopsis thaliana. Molecular Plant Microbe Interactions, 24(4):395-407. doi: 10.1094/MPMI-05-10-0115 URL
[7]	Eamens A L, Smith N A, Curtin S J, Wang M B, Waterhouse P M. 2009. The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes. Cold Spring Harbor Laboratory Press, 15(12):2219-2235.
[8]	Eamens A L, Kim K W, Curtin S J, Waterhouse P M. 2012a. DRB2 is required for microRNA biogenesis inArabidopsis thaliana. PLoS ONE, 7(4):e35933. doi: 10.1371/journal.pone.0035933 URL
[9]	Eamens A L, Wook K K, Waterhouse P M. 2012b. DRB2,DRB3 and DRB5 function in a non-canonical microRNA pathway inArabidopsis thaliana. Plant Signaling & Behavior, 7(10):1224-1229.
[10]	Feng L, Xia R, Liu Y L. 2019. Comprehensive characterization of miRNA and PHAS loci in the diploid strawberry(Fragaria vesca)genome. Horticultural Plant Journal, 5(6):255-267. doi: 10.1016/j.hpj.2019.11.004
[11]	Fukudome A, Kanaya A, Egami M, Nakazawa Y, Hiraguri A, Moriyama H, Fukuhara T. 2011. Specific requirement of DRB4,a dsRNA-binding protein,for the in vitro dsRNA-cleaving activity of Arabidopsis Dicer-like 4. RNA, 17:750-760. doi: 10.1261/rna.2455411 pmid: 21270136
[12]	Glazebrook J. 2005. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens-annual review of phytopathology. Peronospora Pseudomonas Erysiphe Alternaria Botrytis, 43(1):205.
[13]	Hiraguri A, Itoh R, Kondo N, Nomura Y, Aizawa D, Murai Y, Koiwa H, Seki M, Shinozaki K, Fukuhara T. 2005. Specific interactions between Dicer-like proteins and HYL1/DRB- family dsRNA-binding proteins inArabidopsis thaliana. Plant Molecular Biology, 57(2):173-188. doi: 10.1007/s11103-004-6853-5 URL
[14]	Jakubiec A, Yang S W, Chua N H. 2011. Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection. The Plant Journal, 69(1):14-25. doi: 10.1111/tpj.2011.69.issue-1 URL
[15]	Joseph L P, Jackson M J, Eamens A L. 2019. DRB1,DRB2 and DRB4 are required for appropriate regulation of the microRNA399/PHOSPHATE2 expression module inArabidopsis thaliana. Plants(Basel), 8(5):124.
[16]	Kiyota E, Okada R, Kondo N, Hiraguri A, Moriyama H, Fukuhara T. 2011. An Arabidopsis RNase III-like protein,AtRTL2,cleaves double-stranded RNAin vitro. Journal of Plant Research, 124(3):405-414. doi: 10.1007/s10265-010-0382-x URL
[17]	Kurihara Y, Takashi Y, Watanabe Y. 2006. The interaction between DCL1 and HYL1 is important for efficient and precise processing of pri-miRNA in plant microRNA biogenesis. RNA(New York), 12(2):206-212.
[18]	Kwon T. 2016. A double-stranded RNA binding protein,HYL1,regulates plant immunity via the jasmonic acid pathway. Journal of Plant Biology, 59(5):506-514. doi: 10.1007/s12374-016-0303-1 URL
[19]	Lai Zhongxiong, Pan Liangzhen, Chen Zhenguang. 1997. Establishment and maintenance of longan embryogenic cell lines. Journal of Fujian Agricultural University,(2):33-40. (in Chinese)
	赖钟雄, 潘良镇, 陈振光. 1997. 龙眼胚性细胞系的建立与保持. 福建农业大学学报,(2):33-40.
[20]	Lim G H, Hoey T, Zhu S, Clavel M, Yu K, Navarre D, Kachroo A, Deragon J M, Kachroo P. 2018. COP1,a negative regulator of photomorphogenesis,positively regulates plant disease resistance via double-stranded RNA binding proteins. PLoS Pathogens, 14(3):e1006894.
[21]	Lin Y L, Lai Z X. 2010. Reference gene selection for qPCR analysis during somatic embryogenesis in longan tree. Plant Science, 178(4):359-365. doi: 10.1016/j.plantsci.2010.02.005 URL
[22]	Lin Y, Min J, Lai R, Wu Z, Chen Y, Yu L, Cheng C, Jin Y, Tian Q, Liu Q, Liu W, Zhang C, Lin L, Zhang D, Thu M, Zhang Z, Liu S, Zhong C, Fang X, Wang J, Yang H, Varshney RK, Yin Y, Lai Z. 2017. Genome-wide sequencing of longan(Dimocarpus longan Lour.)provides insights into molecular basis of its polyphenol-rich characteristics. Gigascience, 6(5):1-14.
[23]	Manavella P A, Hagmann J, Ott F, Laubinger S, Franz M, Macek B, Weigel D. 2012. Fast-forward genetics identifies plant CPL phosphatases as regulators of miRNA processing factor HYL1. Cell, 151(4):859-870. doi: S0092-8674(12)01238-X pmid: 23141542
[24]	Masliah G, Barraud P, Allain F H. 2013. RNA recognition by double-stranded RNA binding domains:a matter of shape and sequence. Cellular and Molecular Life Sciences, 70(11):1875-1895.
[25]	Montavon T, Kwon Y, Zimmermann A, Hammann P, Vincent T, Cognat V, Bergdoll M, Michel F, Dunoyer P. 2018. Characterization of DCL4 missense alleles provides insights into its ability to process distinct classes of dsRNA substrates. The Plant Journal, 95(2):204-218. doi: 10.1111/tpj.13941 pmid: 29682831
[26]	Morel G M. 1960. Producing virus-free Cymbidium. American Orchid Soc Bulletin, 29(1):495-497.
[27]	Pélissier T, Clavel M, Chaparro C, Pouch-Pélissier M N, Vaucheret H, Deragon J M. 2011. Double-stranded RNA binding proteins DRB2 and DRB4 have an antagonistic impact on polymerase IV-dependent siRNA levels inArabidopsis. RNA(New York), 17(8):1502-1510.
[28]	Ren Yi-ran, Zhao Qiang, Zhao Xian-yan, Hao Yu-jin, You Chun-xiang. 2016. Expression and function analysis of apple MdDRB1 gene . Acta Horticulturae Sinica, 43(6):1033-1043. (in Chinese)
	任怡然, 赵强, 赵先炎, 郝玉金, 由春香. 2016. 苹果MdDRB1的表达与功能分析. 园艺学报, 43(6):1033-1043.
[29]	Sawano H, Matsuzaki T, Usui T, Tabara M, Fukudome A, Kanaya A, Tanoue D, Hiraguri A, Horiguchi G, Ohtani M, Demura T, Kozaki T, Ishii K, Moriyama H, Fukuhara T. 2017. Double-stranded RNA-binding protein DRB3 negatively regulates anthocyanin biosynthesis by modulating PAP1 expression in Arabidopsis thaliana. Journal of Plant Research, 130(1):45-55. doi: 10.1007/s10265-016-0886-0 URL
[30]	Tang Xiangrong, Wu Hao, Jia Ming, Yu Xuhong, He Yuke. 2002. Isolation and expressional analysis of cDNA encoding a dsRNA binding protein homologue OsRBPof rice . Journal of Plant Physiology and Molecular Biology, 28(1):41-45. (in Chinese)
	唐向荣, 吴昊, 贾明, 余旭红, 何雨科. 2002. 水稻双链RNA结合蛋白同源基因OsRBP的克隆及其表达的分析. 植物生理与分子生物学学报,(1):41-45.
[31]	Vicente R S, Plasencia J. 2011. Salicylic acid beyond defence:its role in plant growth and development. J Exp Bot, 62(10):3321-3338. doi: 10.1093/jxb/err031 URL
[32]	Wang Liping, Jiang Lijuan, Ma Fengwang, Guan Qingmei. 2018. Expression and function analysis of MdHYL1gene in apple under drought tolerance . Journal of Northwest A & F University(Natural Science Edition), 46(7):109-116. (in Chinese)
	王丽平, 姜丽娟, 马锋旺, 管清美. 2018. 苹果HYL1基因在干旱中的表达与功能分析. 西北农林科技大学学报(自然科学版), 46(7):109-116.
[33]	Wu Mei-ting, Yang Xiao-yu, Luo Lin-lin, Mo Bei-xin, Liu Lin. 2018. Recent advances in plant microRNA responses to abiotic stresses. Guangdong Agricultural Sciences, 45(3):69-80. (in Chinese)
	吴美婷, 杨晓玉, 罗淋淋, 莫蓓莘, 刘琳. 2018. 植物microRNA响应非生物胁迫研究进展. 广东农业科学, 45(3):69-80.
[34]	Xavier A, Taline E, Dominique L, Stéphanie B, Nicolas B, Virginie G, Hervé V. 2006. DRB4-dependent TAS3 trans-acting siRNAs control leaf morphology through AGO7. Current Biology, 16(9):927-32 pmid: 16682354
[35]	Yang S W, Chen H Y, Yang J, Machida S, Chua N H, Yuan Y A. 2010. Structure of Arabidopsis HYPONASTIC LEAVES1 and its molecular implications for miRNA processing. Structure, 18:594-605. doi: 10.1016/j.str.2010.02.006 URL
[36]	You Chun-Xiang. 2010. Cloning and characterization of appleMdDRB gene as well as the extopic expression ofArabidopsis AtmiR393a gene in tobacco[Ph. D. Dissertation]. Tai’an:Shandong Agricultural University. (in Chinese)
	由春香. 2010. 苹果MdDRB基因的克隆鉴定和拟南芥AtmiR393α基因在烟草中的异位表达分析[博士论文]. 泰安:山东农业大学.
[37]	You C X, Zhao Q, Wang X F, Xie X B, Feng X M, Zhao L L, Shu H R, Hao Y J. 2014. A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture in apple. Plant Biotechnol J, 12(2):183-192. doi: 10.1111/pbi.12125 URL
[38]	Zhao Xian-yan. 2015. Functional characterization of MdDRB1 and the two related MircoRNA genes[M. D. Dissertation]. Tai’an;Shandong Agricultural University. (in Chinese)
	赵先炎. 2015. 苹果MdDRB1基因及2个相关MircoRNAs编码基因的功能鉴定[硕士论文]. 泰安:山东农业大学.
[39]	Zhu S, Jeong R D, Lim G H, Yu K, Wang C, Chandra-Shekara A C, Navarre D, Klessig D F, Kachroo A, Kachroo P. 2013. Double-stranded RNA-binding protein 4 is required for resistance signaling against viral and bacterial pathogens. Cell Reports, 4(6):1168-1184. doi: 10.1016/j.celrep.2013.08.018 URL

基因名称 Gene name	引物名称 Primer name	引物序列(5′-3′) Sequence
DlDRB1	Dlo_011608.1	F:CGGGAACTCAGCAAGAAG;R:ACCAACCATGTGCTCAGTC
DlDRB2	Dlo_032918.1	F:GCCGCAATACACAACAGTC;R:TCGAGATGTCCAAGCTGC
DlDRB3A	Dlo_006566.1	F:ATCTGCTGCTTTGGATGG;R:TGCAATAGGTGGTGGTCTG
DlDRB3B	Dlo_004997.1	F:AGATTCTGGTTTTAGGCTCG;R:GGCCC CAGTTCTCATAGTC
DlDRB3C	Dlo_031650.1	F:AATCAGCACAGATGGACCC;R:CAGGGGGAGCAAATGAAG
DlDRB4A	Dlo_006595.1	F:CAACCATCCCTCCAAGAC;R:CATTGACCTCAGGAGCTTG
DlDRB4B	Dlo_010748.1	F:CACCTCACATGCCGACTTTT;R:TCTCCAACACTCTGCCCAAT
DlDRB6	Dlo_015255.1	F:CGCTGCGTAAAGTGAAGG;R:CCCACAGACAGCTTGCAC

基因名称 Gene name	引物名称 Primer name	引物序列(5′-3′) Sequence
DlDRB1	Dlo_011608.1	F:CGGGAACTCAGCAAGAAG;R:ACCAACCATGTGCTCAGTC
DlDRB2	Dlo_032918.1	F:GCCGCAATACACAACAGTC;R:TCGAGATGTCCAAGCTGC
DlDRB3A	Dlo_006566.1	F:ATCTGCTGCTTTGGATGG;R:TGCAATAGGTGGTGGTCTG
DlDRB3B	Dlo_004997.1	F:AGATTCTGGTTTTAGGCTCG;R:GGCCC CAGTTCTCATAGTC
DlDRB3C	Dlo_031650.1	F:AATCAGCACAGATGGACCC;R:CAGGGGGAGCAAATGAAG
DlDRB4A	Dlo_006595.1	F:CAACCATCCCTCCAAGAC;R:CATTGACCTCAGGAGCTTG
DlDRB4B	Dlo_010748.1	F:CACCTCACATGCCGACTTTT;R:TCTCCAACACTCTGCCCAAT
DlDRB6	Dlo_015255.1	F:CGCTGCGTAAAGTGAAGG;R:CCCACAGACAGCTTGCAC

基因名 Gene name	基因ID Accession No.	CDS/bp	氨基酸数/aa Amino acid	pI	分子量/ kD Mw	染色体位置 Genomic location	注释到拟南芥中 Annotated members of Arabidopsis thaliana	糖基化位点 N-glycosyl sites	亚细胞定位 Subcellular location
DlDRB1	Dlo_011608.1	1 164	387	6.68	41 832.07	scaffold2177;19929;24057;+	AT1G09700.1 (DRB1)	1	线粒体Mitochondrial
DlDRB2	Dlo_032918.1	1 389	462	9.14	50 375.63	scaffold905;239727;242523;-	AT12G28380.1 (DRB2)	1	细胞质Cytoplasmic
DlDRB3A	Dlo_006566.1	1 431	476	9.45	52 055.49	scaffold1488;15186;16811;+	AT3G26932.1 (DRB3)	4	细胞质Cytoplasmic
DlDRB3B	Dlo_004997.1	1 716	571	9.09	63 187.06	scaffold1339;54033;56503;+	AT3G26932.1 (DRB3)	4	细胞质Cytoplasmic
DlDRB3C	Dlo_031650.1	1 749	582	8.66	63 734.75	scaffold839;144409;147126;-	AT3G26932.1 (DRB3)	2	细胞质Cytoplasmic
DlDRB4A	Dlo_006595.1	801	266	6.20	29 386.63	scaffold149;147015;151451;-	AT3G62800.1 (DRB4)	0	细胞核Nuclear
DlDRB4B	Dlo_010748.1	1 599	532	6.45	58 942.26	scaffold202;352210;357863;-	AT3G62800.1 (DRB4)	0	细胞核Nuclear
DlDRB6	Dlo_015255.1	1 371	456	9.42	48 959.58	scaffold288;75960;78995;+	无 No result	2	细胞核Nuclear

基因名 Gene name	基因ID Accession No.	CDS/bp	氨基酸数/aa Amino acid	pI	分子量/ kD Mw	染色体位置 Genomic location	注释到拟南芥中 Annotated members of Arabidopsis thaliana	糖基化位点 N-glycosyl sites	亚细胞定位 Subcellular location
DlDRB1	Dlo_011608.1	1 164	387	6.68	41 832.07	scaffold2177;19929;24057;+	AT1G09700.1 (DRB1)	1	线粒体Mitochondrial
DlDRB2	Dlo_032918.1	1 389	462	9.14	50 375.63	scaffold905;239727;242523;-	AT12G28380.1 (DRB2)	1	细胞质Cytoplasmic
DlDRB3A	Dlo_006566.1	1 431	476	9.45	52 055.49	scaffold1488;15186;16811;+	AT3G26932.1 (DRB3)	4	细胞质Cytoplasmic
DlDRB3B	Dlo_004997.1	1 716	571	9.09	63 187.06	scaffold1339;54033;56503;+	AT3G26932.1 (DRB3)	4	细胞质Cytoplasmic
DlDRB3C	Dlo_031650.1	1 749	582	8.66	63 734.75	scaffold839;144409;147126;-	AT3G26932.1 (DRB3)	2	细胞质Cytoplasmic
DlDRB4A	Dlo_006595.1	801	266	6.20	29 386.63	scaffold149;147015;151451;-	AT3G62800.1 (DRB4)	0	细胞核Nuclear
DlDRB4B	Dlo_010748.1	1 599	532	6.45	58 942.26	scaffold202;352210;357863;-	AT3G62800.1 (DRB4)	0	细胞核Nuclear
DlDRB6	Dlo_015255.1	1 371	456	9.42	48 959.58	scaffold288;75960;78995;+	无 No result	2	细胞核Nuclear

Genome-wide Identification and Expression Analysis of DRB Gene Family in Dimocarpus longan

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 39

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YU Tingting, LI Huan, NING Yuansheng, SONG Jianfei, PENG Lulin, JIA Junqi, ZHANG Weiwei, and YANG Hongqiang. Genome-wide Identification of GRAS Gene Family in Apple and Expression Analysis of Its Response to Auxin [J]. Acta Horticulturae Sinica, 2023, 50(2): 397-409.
[2]	YUAN Xin, XU Yunhe, ZHANG Yupei, SHAN Nan, CHEN Chuying, WAN Chunpeng, KAI Wenbin, ZHAI Xiawan, CHEN Jinyin, GAN Zengyu. Studies on AcAREB1 Regulating the Expression of AcGH3.1 During Postharvest Ripening of Kiwifruit [J]. Acta Horticulturae Sinica, 2023, 50(1): 53-64.
[3]	ZHAO Xueyan, WANG Qi, WANG Li, WANG Fangyuan, WANG Qing, LI Yan. Comparative Transcriptome Analysis of Differential Expression in Different Tissues of Corydalis yanhusuo [J]. Acta Horticulturae Sinica, 2023, 50(1): 177-187.
[4]	GAO Yanlong, WU Yuxia, ZHANG Zhongxing, WANG Shuangcheng, ZHANG Rui, ZHANG De, WANG Yanxiu. Bioinformatics Analysis of Apple ELO Gene Family and Its Expression Analysis Under Low Temperature Stress [J]. Acta Horticulturae Sinica, 2022, 49(8): 1621-1636.
[5]	QIU Ziwen, LIU Linmin, LIN Yongsheng, LIN Xiaojie, LI Yongyu, WU Shaohua, YANG Chao. Cloning and Functional Analysis of the MbEGS Gene from Melaleuca bracteata [J]. Acta Horticulturae Sinica, 2022, 49(8): 1747-1760.
[6]	ZHENG Lin, WANG Shuai, LIU Yunuo, DU Meixia, PENG Aihong, HE Yongrui, CHEN Shanchun, ZOU Xiuping. Gene Cloning and Expression Analysis of NAC Gene in Citrus in Response to Huanglongbing [J]. Acta Horticulturae Sinica, 2022, 49(7): 1441-1457.
[7]	QIAN Jieyu, JIANG Lingli, ZHENG Gang, CHEN Jiahong, LAI Wuhao, XU Menghan, FU Jianxin, ZHANG Chao. Identification and Expression Analysis of MYB Transcription Factors Regulating the Anthocyanin Biosynthesis in Zinnia elegans and Function Research of ZeMYB9 [J]. Acta Horticulturae Sinica, 2022, 49(7): 1505-1518.
[8]	MA Weifeng, LI Yanmei, MA Zonghuan, CHEN Baihong, MAO Juan. Identification of Apple POD Gene Family and Functional Analysis of MdPOD15 Gene [J]. Acta Horticulturae Sinica, 2022, 49(6): 1181-1199.
[9]	ZHANG Kai, MA Mingying, WANG Ping, LI Yi, JIN Yan, SHENG Ling, DENG Ziniu, MA Xianfeng. Identification of HSP20 Family Genes in Citrus and Their Expression in Pathogen Infection Responses Citrus Canker [J]. Acta Horticulturae Sinica, 2022, 49(6): 1213-1232.
[10]	LIANG Chen, SUN Ruyi, XIANG Rui, SUN Yimeng, SHI Xiaoxin, DU Guoqiang, WANG Li. Genome-wide Identification of Grape GRF Family and Expression Analysis [J]. Acta Horticulturae Sinica, 2022, 49(5): 995-1007.
[11]	XIAO Xuechen, LIU Mengyu, JIANG Mengqi, CHEN Yan, XUE Xiaodong, ZHOU Chengzhe, WU Xingjian, WU Junnan, GUO Yinsheng, YEH Kaiwen, LAI Zhongxiong, LIN Yuling. Whole-genome Identification and Expression Analysis of SNAT,ASMT and COMT Families of Melatonin Synthesis Pathway in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(5): 1031-1046.
[12]	GAO Weilin, ZHANG Liman, XUE Chaoling, ZHANG Yao, LIU Mengjun, ZHAO Jin. Expression of E-type MADS-box Genes in Flower and Fruits and Protein Interaction Analysis in Chinese Jujube [J]. Acta Horticulturae Sinica, 2022, 49(4): 739-748.
[13]	WANG Dan, WANG Mi, LIU Jun, ZHOU Xiaohui, LIU Songyu, YANG Yan, ZHUANG Yong. Cloning of U6 Promoters and Establishment of CRISPR/Cas9 Mediated Gene Editing System in Eggplant [J]. Acta Horticulturae Sinica, 2022, 49(4): 791-800.
[14]	LIU Mengyu, JIANG Mengqi, CHEN Yan, ZHANG Shuting, XUE Xiaodong, XIAO Xuechen, LAI Zhongxiong, LIN Yuling. Genome-wide Identification and Expression Analysis of GDSL Esterase/Lipase Genes in Dimocarpus longan [J]. Acta Horticulturae Sinica, 2022, 49(3): 597-612.
[15]	CHEN Jianxin, WEI Yuqian, TANG Jie, ZHU Yongjin, MA Huancheng, WU Jianrong. Molecular Detection and Sequence Analysis of ORSV and CymMV in Cultivated Cymbidium goeringii in Dali,Yunnan [J]. Acta Horticulturae Sinica, 2022, 49(3): 655-662.