Review of Base Editing Technology and its Application in Crop Genetic Improvement

doi:10.16420/j.issn.0513-353x.2020-0540

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (4): 719-732.doi: 10.16420/j.issn.0513-353x.2020-0540

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Review of Base Editing Technology and its Application in Crop Genetic Improvement

LI Guobin, AI Guo, WEI Jing, ZHANG Junhong()

Key Laboratory of Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,College of Horticulture and Forestry Sciences,Wuhan 430070,China

Received:2020-12-23 Online:2021-04-25 Published:2021-04-29
Contact: ZHANG Junhong E-mail:zhangjunhng@mail.hzau.edu.cn

Abstract

Abstract:

The base editing is a new technology developed on the basis of the CRISPR/Cas system that can precisely edit the genome. It includes cytosine base editor（CBE）,adenine base editor（ABE）and the latest prime editing（PE）. The cytosine base editor can convert C/G to T/A at the target site of the genome,the adenine base editor can convert the A/T to G/C,while the prime editing can realize all 12 types nucleotides replacing（C-T,G-A,A-G,T-C,C-A,C-G,G-C,G-T,A-C,A-T,T-A,T-G）,and the bases insertion and deletion. This article makes a systematic introduction of the principles,development processes,respective advantages and disadvantages of the three base editors,and their applications and developments in crop genetic improvement. Meanwhile,It looks forward to the application prospects of base editing technology in crop breeding.

Key words: base editing, cytosine base editor（CBE）, adenine base editor（ABE）, prime editing（PE）, crop development

CLC Number:

LI Guobin, AI Guo, WEI Jing, ZHANG Junhong. Review of Base Editing Technology and its Application in Crop Genetic Improvement[J]. Acta Horticulturae Sinica, 2021, 48(4): 719-732.

Figures/Tables 3

References 63

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物种 Crop name	编辑系统 Base editor	编辑形式 Editor type	PAM	靶基因 Targeted gene	应用 Function	参考文献 Reference
水稻Rice	BE3, Anc689BE4max	C-T	NGG,NG	NRT1.1b,SLR1	氮利用率、株形 Nitrogen use efficiency,Plant architecture	Lu & Zhu, 2017;Wang et al.2019
	CBE-P1-P5, ABE-P1-P5	C-T,A-G	NGG	OsSPL13, OsSPL14, OsSPL16, OsSPL17, OsSPL18, GRF4, OsTOE1, OslDS1, OMTN1, SNB, PMS1, PMS3	株形,产量,抗性 Plant architecture,Yield increase,Improve resistance	Hua et al.2019
	ABE-P1S, ABEmax	A-G	NG,NGG	OsSPL14, OsSPL16, OsSERK2, SLR1, Tms9-1, OsNRT1.1B, OsACC1, OsDEP1, OsSPL17, OsSPL18, SLR1, ALS, EPSPS,	株形,抗除草剂 Plant architecture,Herbicide resistance	Hua et al.2018,2020b;Wang et al.2019
	rBE3,rBE4, rBE5,rBE9	C-T	NGG, NAG, NGA, AGTG, AGCG AGTG, AGCG	OsSERK1, OsSERK2, OsAOS1, OsJAR1, OsJAR2, OsCOL2		Ren et al.2017,2018
	rBE14	A-G	NGG	OsMPK6, OsMPK13, OsSERK2, OsWRKY45, Tms9-1	抗病Pathogen resistance	Yan et al.2018
	rBE22, rBE25	C-T	NG, NAC, NTG, NTT, NCG	OsBZR1		Ren et al.2019;Zhong et al.2019;Wang et al.2020
	rBE23, rBE26	A-G	NG, NAC, NTG, NTT, NCG	OsCERK2, OsGS1	抗病 Pathogen resistance	Ren et al.2019;Wang et al.2020
	pCXUN-BE3	C-T	NGG	OsPDS, OsSBEIIb	品质 Nutritional improvement	Li et al.2017
	A3A-PBE	C-T	NGG, NGA, NGCG, NNGRRT, NNNRRT	OsAAT-T1, OsCDC48, OsDEP1, sNRT1.1B-T1, OsOD, OsEV		Zong et al.2018
	xCas9-epBE	C-T	GA, NG	OsMPK2, OsMPK5, OsALS, OsNRT1.1B, OsWaxy	抗病, 抗除草剂 Pathogen resistance, Herbicide resistance	Zhong et al.2019;Zhang et al.2020
小麦Wheat	A3A-PBE	C-T	NGG, NGA, NGCG, NNGRRT, NNNRRT	TaALS, TaMTL, TaLOX2-T1, TaLOX2-T2	抗除草剂 Herbicide resistance	Zong et al.2018
玉米Maize	PBE（BE3）	C-T	NGG	ZmCENH3		Zong et al.2017
马铃薯 Potato	A3A-PBE	C-T	NGG, NGA, NGCG, NNGRRT, NNNRRT	StGBSS-T6		Zong et al.2018
	CBE	C-T	NGG	StGBSS1	淀粉含量 Starch synthesis	Veillet et al.2019a
拟南芥 Arabidopsis	BE3	C-T	NGG	AtALS	抗除草剂 Herbicide resistance	Chen et al.2017
番茄Tomato	CBE	C-T	NGG	ALS	抗除草剂 Herbicide resistance	Veillet et al.2019b
	Target-AID	C-T	NGG	DELLA, ETR1	株型 Plant architecture	Shimatani et al.2017
油菜Rape	pcABE7.10	A-G	NGG	BnALS, BnPDS	抗除草剂 Herbicide resistance	Kang et al.2018
	CBE	C-T	NGG	BnALS1, BnALS3	抗除草剂 Herbicide resistance	Wu et al.2020
棉花Cotton	GhBE3	C-T	NGG	GhCAL, GhPEPB	株型 Plant architecture	Qin et al.2020
西瓜Watermelon	BE3	C-T	NGG	ClALS	抗除草剂 Herbicide resistance	Tian et al.2018

物种 Crop name	编辑系统 Base editor	编辑形式 Editor type	PAM	靶基因 Targeted gene	应用 Function	参考文献 Reference
水稻Rice	BE3, Anc689BE4max	C-T	NGG,NG	NRT1.1b,SLR1	氮利用率、株形 Nitrogen use efficiency,Plant architecture	Lu & Zhu, 2017;Wang et al.2019
	CBE-P1-P5, ABE-P1-P5	C-T,A-G	NGG	OsSPL13, OsSPL14, OsSPL16, OsSPL17, OsSPL18, GRF4, OsTOE1, OslDS1, OMTN1, SNB, PMS1, PMS3	株形,产量,抗性 Plant architecture,Yield increase,Improve resistance	Hua et al.2019
	ABE-P1S, ABEmax	A-G	NG,NGG	OsSPL14, OsSPL16, OsSERK2, SLR1, Tms9-1, OsNRT1.1B, OsACC1, OsDEP1, OsSPL17, OsSPL18, SLR1, ALS, EPSPS,	株形,抗除草剂 Plant architecture,Herbicide resistance	Hua et al.2018,2020b;Wang et al.2019
	rBE3,rBE4, rBE5,rBE9	C-T	NGG, NAG, NGA, AGTG, AGCG AGTG, AGCG	OsSERK1, OsSERK2, OsAOS1, OsJAR1, OsJAR2, OsCOL2		Ren et al.2017,2018
	rBE14	A-G	NGG	OsMPK6, OsMPK13, OsSERK2, OsWRKY45, Tms9-1	抗病Pathogen resistance	Yan et al.2018
	rBE22, rBE25	C-T	NG, NAC, NTG, NTT, NCG	OsBZR1		Ren et al.2019;Zhong et al.2019;Wang et al.2020
	rBE23, rBE26	A-G	NG, NAC, NTG, NTT, NCG	OsCERK2, OsGS1	抗病 Pathogen resistance	Ren et al.2019;Wang et al.2020
	pCXUN-BE3	C-T	NGG	OsPDS, OsSBEIIb	品质 Nutritional improvement	Li et al.2017
	A3A-PBE	C-T	NGG, NGA, NGCG, NNGRRT, NNNRRT	OsAAT-T1, OsCDC48, OsDEP1, sNRT1.1B-T1, OsOD, OsEV		Zong et al.2018
	xCas9-epBE	C-T	GA, NG	OsMPK2, OsMPK5, OsALS, OsNRT1.1B, OsWaxy	抗病, 抗除草剂 Pathogen resistance, Herbicide resistance	Zhong et al.2019;Zhang et al.2020
小麦Wheat	A3A-PBE	C-T	NGG, NGA, NGCG, NNGRRT, NNNRRT	TaALS, TaMTL, TaLOX2-T1, TaLOX2-T2	抗除草剂 Herbicide resistance	Zong et al.2018
玉米Maize	PBE（BE3）	C-T	NGG	ZmCENH3		Zong et al.2017
马铃薯 Potato	A3A-PBE	C-T	NGG, NGA, NGCG, NNGRRT, NNNRRT	StGBSS-T6		Zong et al.2018
	CBE	C-T	NGG	StGBSS1	淀粉含量 Starch synthesis	Veillet et al.2019a
拟南芥 Arabidopsis	BE3	C-T	NGG	AtALS	抗除草剂 Herbicide resistance	Chen et al.2017
番茄Tomato	CBE	C-T	NGG	ALS	抗除草剂 Herbicide resistance	Veillet et al.2019b
	Target-AID	C-T	NGG	DELLA, ETR1	株型 Plant architecture	Shimatani et al.2017
油菜Rape	pcABE7.10	A-G	NGG	BnALS, BnPDS	抗除草剂 Herbicide resistance	Kang et al.2018
	CBE	C-T	NGG	BnALS1, BnALS3	抗除草剂 Herbicide resistance	Wu et al.2020
棉花Cotton	GhBE3	C-T	NGG	GhCAL, GhPEPB	株型 Plant architecture	Qin et al.2020
西瓜Watermelon	BE3	C-T	NGG	ClALS	抗除草剂 Herbicide resistance	Tian et al.2018

Review of Base Editing Technology and its Application in Crop Genetic Improvement

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