Characteristic and Relative Expression Pattern Analysis of FWL/PLAC8 Family in Blueberry

doi:10.16420/j.issn.0513-353x.2020-0151

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 336-346.doi: 10.16420/j.issn.0513-353x.2020-0151

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Characteristic and Relative Expression Pattern Analysis of FWL/PLAC8 Family in Blueberry

YU Lei¹, ZHOU Ya¹, ZONG Yu¹^,², ZHANG Ying¹, QIU Jiaqi¹, LI Yongqiang¹^,², YANG Li¹^,²^,^*(), GUO Weidong¹^,²^,^*()

¹College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004,China
²Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants,Zhejiang Normal University,Jinhua,Zhejiang 321004,China

Received:2020-05-08 Revised:2020-08-25 Online:2021-02-25 Published:2021-03-09
Contact: YANG Li,GUO Weidong E-mail:yangli@zjnu.edu.cn;gwd@zjnu.cn

Abstract

Abstract:

Fruit size and weight are the most vital agronomic characters for consumers,breeders and researchers,and fruit weight（FW）was reported as an important quantitative trait locus affecting tomato and other fruit weight and size remarkably. In this study,the growth curves during Vaccinium corymbosum ‘O’Neal’（large-size fruit）and‘Bluerain’（small-size fruit）flower bud enlargement and fruit development,and conventionally physical indexes of mature fruits were measured and analyzed. The FWL family with conserved domain PLAC8 in the blueberry genome was identified,and the gene structure, chromosome location,conserved motifs of VcFWL/PLAC8 were analyzed. The relative expression level of VcFWL/PLAC8 genes during‘O’Neal’and‘Bluerain’flower bud enlargement and fruit development was detected by real-time fluorescence quantitative PCR. The results showed that the fruit weight of‘O’Neal’was dramatically higher than that of‘Bluerain’from stage S2,and the locule number,seed number and weight per fruit of‘O’Neal’mature fruits were also notably higher than those of‘Bluerain’. The VcFWL/PLAC8 family contained 11 members with highly conserved motifs in the‘Draper’genome,which can be divided into three clusters. qRT-PCR analysis showed that the expression patterns of A and C subfamilies were consistant with opposite to the cell proliferation trend,while the expression level of B subfamily was relatively low. These results suggested that VcFWL/PLAC8 genes might be involved in fruit growth and development through different regulatory mechanisms and pathways.

Key words: blueberry, FWL/PLAC8, Vaccinium corymbosum, fruit size, fruit development

CLC Number:

S663.9

YU Lei, ZHOU Ya, ZONG Yu, ZHANG Ying, QIU Jiaqi, LI Yongqiang, YANG Li, GUO Weidong. Characteristic and Relative Expression Pattern Analysis of FWL/PLAC8 Family in Blueberry[J]. Acta Horticulturae Sinica, 2021, 48(2): 336-346.

Figures/Tables 8

References 41

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引物名称 Primer name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer	扩增长度/bp Predicted length
A subfamily	TCAGACATCCTTCCCACTAACC	CCGTCGCAGGAGACGTAG	138
B subfamily	CCAGGGTTTCGCTCTCCTTCC	AGCTCCCTTGGCCTGCGG	186
C subfamily	TCTGCGAAAGGTCTGGAGAT	CATGGCAACCAACATTTCAG	285
VcGAPDH	CGGCTACTTACGAGCAAATCAA	TTCAGTGTAGCCCAAAATTCCTTT	80

引物名称 Primer name	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer	扩增长度/bp Predicted length
A subfamily	TCAGACATCCTTCCCACTAACC	CCGTCGCAGGAGACGTAG	138
B subfamily	CCAGGGTTTCGCTCTCCTTCC	AGCTCCCTTGGCCTGCGG	186
C subfamily	TCTGCGAAAGGTCTGGAGAT	CATGGCAACCAACATTTCAG	285
VcGAPDH	CGGCTACTTACGAGCAAATCAA	TTCAGTGTAGCCCAAAATTCCTTT	80

品种 Cultivar	百粒种子质量/g Weight / 100 seeds	单果种子数 Seed number	单果种子质量/g Seed weight pre fruit	心室数比例/% Locule number
品种 Cultivar	百粒种子质量/g Weight / 100 seeds	单果种子数 Seed number	单果种子质量/g Seed weight pre fruit	4	5	6	7
奥尼尔O’Neal	0.04 ± 0.00	83.00 ± 4.06^**	0.036 ± 0.007^**		57.39	21.09	16.52
蓝雨Bluerain	0.04 ± 0.00	30.42 ± 8.31	0.012 ± 0.004	1.64	98.36

品种 Cultivar	百粒种子质量/g Weight / 100 seeds	单果种子数 Seed number	单果种子质量/g Seed weight pre fruit	心室数比例/% Locule number
品种 Cultivar	百粒种子质量/g Weight / 100 seeds	单果种子数 Seed number	单果种子质量/g Seed weight pre fruit	4	5	6	7
奥尼尔O’Neal	0.04 ± 0.00	83.00 ± 4.06^**	0.036 ± 0.007^**		57.39	21.09	16.52
蓝雨Bluerain	0.04 ± 0.00	30.42 ± 8.31	0.012 ± 0.004	1.64	98.36

基因名称 Gene name	染色体位置 Chromosome location	CDS/bp	外显子数 Exon number	大小/aa Size	分子量/kD Molecular weight	等电点 pI
gene-169.8	VaccDscaff5：16978476..16983996-	561	3	186	20.39	5.60
gene-244.19	VaccDscaff8：24434249..24439769+	693	2	230	25.72	4.92
gene-228.26	VaccDscaff10：22828768..22834442+	672	4	223	24.22	5.35
gene-180.23	VaccDscaff1：18011712..18017230-	777	3	258	27.84	8.12
gene-48.40	VaccDscaff27：4876429..4880014+	741	3	246	26.38	5.40
gene-85.17	VaccDscaff17：8569670..8572170+	657	2	218	23.42	8.54
gene-85.18	VaccDscaff17：8572400..8573369+	396	2	131	14.60	6.27
gene-305.30	VaccDscaff34：30578948..30582561-	738	3	246	26.40	5.37
gene-296.30	VaccDscaff29：29591240..29601268-	603	3	200	21.34	4.76
gene-308.41	VaccDscaff26：30828379..30838346-	603	3	200	21.34	4.76
gene-316.28	VaccDscaff21：31596223..31606319-	603	3	200	21.30	4.76
VcFW2.2	v.corymbosum_GDV_reftransV1_0004680	732	/	243	26.32	5.97

Characteristic and Relative Expression Pattern Analysis of FWL/PLAC8 Family in Blueberry

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