The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development

doi:10.16420/j.issn.0513-353x.2020-0388

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 289-299.doi: 10.16420/j.issn.0513-353x.2020-0388

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The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development

XIE Guofang¹^,², LIU Na², SONG Yi², GUAN Chunhua², ZHANG Mingsheng¹^,^*()

¹Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region（Ministry of Education）,Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering（CICMEAB）,College of Life Sciences/Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,China
²Food and Pharmaceutical Engineering Institute/Guizhou Engineering Research Center for Fruit Processing,Guiyang University,Guiyang 550005,China

Received:2020-07-01 Revised:2020-11-03 Online:2021-02-25 Published:2021-03-09
Contact: ZHANG Mingsheng E-mail:mshzhang@163.com

Abstract

Abstract:

The cell wall metabolism of fresh common bean（Phaseolus vulgaris L.）seriously affects the edible quality. The contents of endogenous hormones,the main components and enzymes related to cell wall metabolism were analyzed in four developmental stages in beans‘Dabaibang’,and the relationship between endogenous hormones and cell wall metabolism was analyzed. The results showed that the content of GA₃ and 6-BA decreased,the content of ethylene（ET）and JA decreased firstly and then increased during the development of pods. The pods was mainly in elongation growth before the 10th day after anthesis,and the pods was mainly in thickening growth after the 10th day after anthesis. Sucrose synthetase（SuSy）promoted sucrose synthesis before 10 days after anthesis. Sucrose content decreased and cellulose content increased with the decrease of SuSy and cellulase（Cx）activity. G-lignin deposition in xylem at 5 days after anthesis,G-lignin and S-lignin were deposited in phloem,gradually deepened and diffused to both sides after the 10th day after anthersis. S-lignin was only deposited in phloem. The high phenylalanine ammonia lyase（PAL）activity provided the substrate for lignin biosynthesis at the 5th day after anthesis,and then the increase of cinnamyl alcohol dehydrogenase（CAD）activity promoted lignin synthesis,the increase of superoxide dismutase（SOD）activity promoted the conversion of $\mathrm{O}_{2}^{\overline{·}}$ to H₂O₂,and the polymerization of lignin with peroxidase（POD）activity. Polygalacturonase（PG）activity,sodium-carbonate-soluble-pectin（SSP）and water-soluble-pectin（WSP）content increased firstly and then decreased,pectin methylesterase（PME）activity decreased,chelator-soluble-pectin（CSP）content decreased firstly and then increased. The correlation analysis showed that JA positively regulated pod development and cellulose metabolism,while ET,GA₃,6-BA and ABA were the opposite. JA regulates lignin polymerization by inhibiting the conversion of $\mathrm{O}_{2}^{\overline{·}}$ to H₂O₂ and POD activity. ET,GA₃,6-BA and ABA promoted lignin synthesis by enhancing PAL and POD activity. IAA and SA were involved in regulation of pectin metabolism.

Key words: Phaseolus vulgaris, bean development, endogenous hormones, cell wall metabolism

CLC Number:

S643.1

XIE Guofang, LIU Na, SONG Yi, GUAN Chunhua, ZHANG Mingsheng. The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development[J]. Acta Horticulturae Sinica, 2021, 48(2): 289-299.

Figures/Tables 7

References 36

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花后天数 Days after anthesis	乙烯释放速率/ （mg · kg^-1· h^-1） ET	ABA/ （μg · g^-1 FW）	GA₃/ （μg · g^-1 FW）	6-BA/ （μg · g^-1 FW）	JA/ （μg · g^-1 FW）	SA/ （μg · g^-1 FW）	IAA/ （μg · g^-1 FW）
5	86.22 ± 1.18 a	0.15 ± 0.00 a	1.42 ± 0.06 a	52.93 ± 1.39 a	1.22 ± 0.02 c	15.82 ± 0.33 b	0.09 ± 0.00 b
10	19.77 ± 0.41 b	0.12 ± 0.00 c	0.47 ± 0.02 b	7.15 ± 0.24 b	1.11 ± 0.00 c	10.98 ± 0.51 c	0.08 ± 0.00 b
15	16.97 ± 0.24 c	0.13 ± 0.00 b	0.30 ± 0.02 c	6.34 ± 0.26 b	2.14 ± 0.05 b	49.31 ± 0.73 a	0.88 ± 0.06 a
20	21.36 ± 0.82 b	0.11 ± 0.00 d	0.14 ± 0.01 d	0.51 ± 0.04 c	2.69 ± 0.04 a	8.50 ± 0.28 d	0.11 ± 0.01 b

花后天数 Days after anthesis	乙烯释放速率/ （mg · kg^-1· h^-1） ET	ABA/ （μg · g^-1 FW）	GA₃/ （μg · g^-1 FW）	6-BA/ （μg · g^-1 FW）	JA/ （μg · g^-1 FW）	SA/ （μg · g^-1 FW）	IAA/ （μg · g^-1 FW）
5	86.22 ± 1.18 a	0.15 ± 0.00 a	1.42 ± 0.06 a	52.93 ± 1.39 a	1.22 ± 0.02 c	15.82 ± 0.33 b	0.09 ± 0.00 b
10	19.77 ± 0.41 b	0.12 ± 0.00 c	0.47 ± 0.02 b	7.15 ± 0.24 b	1.11 ± 0.00 c	10.98 ± 0.51 c	0.08 ± 0.00 b
15	16.97 ± 0.24 c	0.13 ± 0.00 b	0.30 ± 0.02 c	6.34 ± 0.26 b	2.14 ± 0.05 b	49.31 ± 0.73 a	0.88 ± 0.06 a
20	21.36 ± 0.82 b	0.11 ± 0.00 d	0.14 ± 0.01 d	0.51 ± 0.04 c	2.69 ± 0.04 a	8.50 ± 0.28 d	0.11 ± 0.01 b

花后天数 Days after anthesis	豆荚长度/cm Pod length	单荚豆粒质量/g Seed weight per pod	豆荚厚度/mm Pod thickness	豆荚直径/mm Pod diameter	单荚质量/g Weight per pod	豆荚相对厚度/% Relative thickness of pod
5	11.90 ± 0.37 c	0 d	2.67 ± 0.02 c	5.34 ± 0.03 d	2.90 ± 0.12 d	100.00 ± 0.00 a
10	19.08 ± 0.09 b	0.70 ± 0.06 c	3.02 ± 0.04 b	9.79 ± 0.16 c	13.60 ± 0.32 c	61.65 ± 0.55 b
15	20.62 ± 0.25 b	1.23 ± 0.07 b	3.15 ± 0.10 b	11.28 ± 0.21 b	18.07 ± 0.47 b	55.86 ± 0.93 c
20	22.41 ± 0.84 a	1.83 ± 0.09 a	3.77 ± 0.07 a	13.76 ± 0.17 a	23.43 ± 1.10 a	54.77 ± 0.74 c

花后天数 Days after anthesis	豆荚长度/cm Pod length	单荚豆粒质量/g Seed weight per pod	豆荚厚度/mm Pod thickness	豆荚直径/mm Pod diameter	单荚质量/g Weight per pod	豆荚相对厚度/% Relative thickness of pod
5	11.90 ± 0.37 c	0 d	2.67 ± 0.02 c	5.34 ± 0.03 d	2.90 ± 0.12 d	100.00 ± 0.00 a
10	19.08 ± 0.09 b	0.70 ± 0.06 c	3.02 ± 0.04 b	9.79 ± 0.16 c	13.60 ± 0.32 c	61.65 ± 0.55 b
15	20.62 ± 0.25 b	1.23 ± 0.07 b	3.15 ± 0.10 b	11.28 ± 0.21 b	18.07 ± 0.47 b	55.86 ± 0.93 c
20	22.41 ± 0.84 a	1.83 ± 0.09 a	3.77 ± 0.07 a	13.76 ± 0.17 a	23.43 ± 1.10 a	54.77 ± 0.74 c

花后天数 Days after anthesis	蔗糖/% Sucrose	SuSy活性/（g · kg^-1 FW） SuSy activity	纤维素/（U · g^-1） Cellulose	Cx活性/（U · g^-1） Cx activity
5	2.24 ± 0.03 b	76.50 ± 2.74 a	349.89 ± 6.01 d	1.80 ± 0.08 a
10	2.71 ± 0.03 a	23.16 ± 1.07 b	523.22 ± 5.74 c	1.77 ± 0.00 a
15	2.06 ± 0.08 bc	20.16 ± 1.03 b	618.07 ± 4.26 a	1.24 ± 0.02 b
20	1.83 ± 0.06 c	20.78 ± 1.26 b	599.41 ± 5.83 b	0.48 ± 0.02 c

The Relationship Between Changes of Endogenous Hormones and Cell Wall Metabolism of Common Bean During Bean Development

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花后天数/d Days after anthesis	总酚/（g · kg^-1 DW） Total phenols	PAL/（U · g^-1）	CAD/（U · g^-1）	$\mathrm{O}_{2}^{\overline{·}}$/ （mmol · min^-1 · kg^-1）	SOD/（U · g^-1）
5	1.21 ± 0.02 c	31.32 ± 1.77 a	47.29 ± 1.97 c	0.44 ± 0.00 a	509.60 ± 7.44 b
10	1.57 ± 0.05 b	24.32 ± 0.89 b	53.18 ± 2.26 c	0.31 ± 0.01 b	562.80 ± 48.52 b
15	1.41 ± 0.03 b	24.65 ± 1.67 b	70.73 ± 1.55 b	0.31 ± 0.01 b	904.80 ± 47.29 a
20	2.11 ± 0.06 a	33.95 ± 1.51 a	128.80 ± 11.59 a	0.28 ± 0.01 b	859.90 ± 43.25 a
花后天数/d Days after anthesis	H₂O₂/（mol · kg^-1）	POD/（U · g^-1）	G木质素/（μg · g^-1 DW） G-lignin	S木质素/（μg · g^-1 DW） S-lignin
5	1.74 ± 0.01 c	368.65 ± 12.52 a	104.65 ± 1.63 a	50.67 ± 1.10 a
10	2.69 ± 0.04 a	315.24 ± 9.71 b	86.27 ± 0.77 c	44.47 ± 0.29 c
15	2.22 ± 0.07 b	301.38 ± 13.97 b	91.63 ± 1.52 b	45.32 ± 0.31 bc
20	2.71 ± 0.03 a	230.33 ± 10.02 c	94.29 ± 0.72 b	48.77 ± 1.79 ab

花后天数/d Days after anthesis	PG /（U · g^-1）	PME /（U · g^-1）	CSP /（g · kg^-1 DW）	SSP /（g · kg^-1 DW）	WSP/（g · kg^-1 DW）
5	203.90 ± 9.08 b	50.74 ± 0.64 a	0.84 ± 0.01 a	0.79 ± 0.01 b	1.15 ± 0.05 b
10	193.40 ± 7.37 b	30.63 ± 0.41 c	0.78 ± 0.03 a	0.86 ± 0.02 b	1.34 ± 0.05 ab
15	445.00 ± 16.27 a	37.36 ± 3.36 b	0.34 ± 0.03 b	1.05 ± 0.04 a	1.42 ± 0.01 a
20	145.90 ± 5.40 c	33.60 ± 1.18 bc	0.84 ± 0.04 a	0.83 ± 0.02 b	0.88 ± 0.06 c

分类 Sorts	指标 Index		ET		GA₃		6-BA		IAA		ABA		SA		JA
发育性状 Developmental traits	豆荚长度 Pod length		-0.934^**		-0.984^**		-0.964^**		0.325		-0.824^**		0.146		0.716^**
	豆荚厚度 Pod thickness		-0.870^**		-0.958^**		-0.931^**		0.257		-0.875^**		0.076		0.822^**
	豆荚相对厚度 Relative thickness of pods		0.987^**		0.986^**		0.993^**		-0.392		0.779^**		-0.227		-0.614^*
	单荚质量 Weight per pods		-0.870^**		-0.955^**		-0.927^**		0.298		-0.857^**		0.116		0.827^**
	单荚豆粒质量 Seed weight per pods		-0.784^**		-0.903^**		-0.859^**		0.279		-0.838^**		0.097		0.896^**
纤维素代谢 Cellulose metabolism	蔗糖Sucrose		-0.694^*		-0.689^*		-0.643^*		0.868^**		-0.250		0.790^**		0.602^*
	蔗糖合成酶 SuSy		-0.306		-0.520		-0.456		-0.253		-0.765^**		-0.398		0.813^**
	纤维素酶 Cellulase		-0.901^**		-0.960^**		-0.928^**		0.496		-0.736^**		0.331		0.803^**
	纤维素Cellulose		-0.945^**		-0.969^**		-0.949^**		0.533		-0.728^**		0.376		0.720^**
木质素代谢 Lignin metabolism	总酚 Total phenols		0.885^**		0.909^**		0.944^**		-0.029		0.954^**		0.144		-0.595^*
	PAL		0.699^*		0.548		0.584^*		-0.507		0.177		-0.464		0.051
	CAD		-0.448		-0.649^*		-0.575		-0.045		-0.771^**		-0.195		0.888^**
	G木质素 G-lignin		0.876^**		0.773^**		0.816^**		-0.192		0.513		-0.105		-0.076
	S木质素 S-lignin		0.669^*		0.546		0.577^*		-0.369		0.194		-0.321		0.068
	SOD		0.172		0.324		0.256		0.075		0.399		0.207		-0.491
	$\mathrm{O}_{2}^{\overline{·}}$		-0.467		-0.652^*		-0.601^*		-0.176		-0.868^**		-0.359		0.802^**
	H₂O₂		0.494		0.620^*		0.614^*		0.472		0.912^**		0.620^*		-0.524
	POD		0.696^*		0.844^**		0.777^**		-0.042		0.803^**		0.115		-0.807^**
果胶代谢 Pectin metabolism	PME		0.894^**		0.850^**		0.892^**		-0.016		0.749^**		0.073		-0.312
	PG		-0.488		-0.318		-0.432		-0.287		-0.343		-0.312		-0.430
	CSP		0.408		0.335		0.305		-0.933^**		-0.173		-0.923^**		-0.228
	SSP		-0.556		-0.477		-0.462		0.907^**		-0.005		0.860^**		0.290
	WSP		-0.143		0.041		0.004		0.510		0.457		0.575		-0.444
分类 Sorts		指标 Index		ABA/IAA		GA₃/IAA		GA₃/ABA		ET/ABA		IAA/ET		GA₃/ET
发育性状 Developmental traits		豆荚长度 Pod length		-0.636^*		-0.960^**		-0.980^**		-0.891^**		-0.921^**		-0.293
		豆荚厚度 Pod thickness		-0.619^*		-0.933^**		-0.957^**		-0.813^**		-0.857^**		-0.456
		豆荚相对厚度 Relative thickness of pods		0.647^*		0.969^**		0.975^**		0.956^**		0.974^**		0.134
		单荚质量 Weight per pods		-0.655^*		-0.939^**		-0.955^**		-0.811^**		-0.855^**		-0.448
		单荚豆粒质量 Seed weight per pods		-0.657^*		-0.898^**		-0.911^**		-0.717^**		-0.767^**		-0.578^*
纤维素代谢 Cellulose metabolism		蔗糖Sucrose		-0.925^**		-0.710^**		-0.809^**		-0.726^**		-0.696^*		-0.070
		蔗糖合成酶 SuSy		-0.198		-0.532		-0.453		-0.207		-0.294		-0.864^**
		纤维素酶 Cellulase		-0.794^**		-0.965^**		-0.985^**		-0.867^**		-0.890^**		-0.342
		纤维素Cellulose		-0.781^**		-0.969^**		-0.991^**		-0.930^**		-0.941^**		-0.202
木质素代谢 Lignin metabolism		总酚 Total phenols		0.376		0.881^**		0.829^**		0.807^**		0.863^**		0.253
		PAL		0.415		0.528		0.564		0.762^**		0.713^**		-0.547
		CAD		-0.401		-0.666^*		-0.613^*		-0.360		-0.437		-0.823^**
		G木质素 G-lignin		0.270		0.747^**		0.698^*		0.899^**		0.880^**		-0.328
		S木质素 S-lignin		0.322		0.534		0.536		0.697^*		0.657^*		-0.422
		SOD		0.172		0.338		0.284		0.169		0.211		0.452
		$\mathrm{O}_{2}^{\overline{·}}$		-0.243		-0.653^*		-0.569		-0.398		-0.479		-0.709^**
		H₂O₂		-0.059		0.601^*		0.465		0.414		0.496		0.498
		POD		0.446		0.860^**		0.794^**		0.651^*		0.693^*		0.579^*
果胶代谢 Pectin metabolism		PME		0.257		0.819^**		0.747^**		0.864^**		0.884^**		-0.027
		PG		0.316		-0.261		-0.171		-0.495		-0.490		0.617^*
		CSP		0.815^**		0.356		0.505		0.480		0.417		-0.290
		SSP		-0.812^**		-0.490		-0.623^*		-0.614^*		-0.559		0.223
		WSP		-0.184		0.038		-0.041		-0.204		-0.117		0.675^*

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