Studies on the Regreening Mechanism of the Iron-deficiency Chlorosis Leaves induced by GA3 in‘Whangkeumbae’（Pyrus pyrifolia Nakai）

doi:10.16420/j.issn.0513-353x.2020-0285

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 254-264.doi: 10.16420/j.issn.0513-353x.2020-0285

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Studies on the Regreening Mechanism of the Iron-deficiency Chlorosis Leaves induced by GA₃ in‘Whangkeumbae’（Pyrus pyrifolia Nakai）

JIA Bing, GUO Guoling, WANG Youyu, YE Zhenfeng, LIU Li, LIU Pu, HENG Wei, ZHU Liwu^*()

College of Horticulture,Anhui Agricultural University,Hefei 230036,China

Received:2020-06-06 Revised:2020-08-27 Online:2021-02-25 Published:2021-03-09
Contact: ZHU Liwu E-mail:zhuliwu@ahau.edu.cn

Abstract

Abstract:

In order to reveal the regreening effect and physiological mechanism of the iron-deficiency chlorosis leaves induced by GA₃,the iron-deficiency chlorosis and normal plants of‘Whangkeumbae’（Pyrus pyrifolia Nakai）were used as the experiment materials,and GA₃ solutions of 50,100,200 and 400 mg · L^-1 was sprayed on chlorosis leaves during the growth period. The contents of Fe,GA₃,GA₄,IAA and JA in leaves were determined,the chloroplast ultrastructure was observed and photographed under an electron microscope,and the expression levels of genes related to Fe metabolism or GA signal transduction were analyzed by fluorescence quantitative PCR. The results showed that on the 9th day after treatment with exogenous GA₃,the chlorosis leaves showed different degrees of regreening,with obvious vein regreening and partial body regreening. On the 12th day of treatment with 400 mg · L^-1 GA₃,the regreening of chlorosis leaves were the most obvious. The Fe content in regreening leaves were significantly higher than that in control with water on the 9th and 12th day after GA₃treatment. Exogenous GA₃ could induce the expressions of FER1,FER2,FER3,FRO2,IRT1 and FD1 in chlorosis leaves. On the 12th day,the expression levels of FER2 and IRT1 in each treatment were more than 10 and 40 times higher than that in the control group,indicating that exogenous GA₃ promoted the expression of genes related to iron storage,reduction and transport in leaves. Exogenous GA₃ treatment significantly increased the contents of endogenous GA₃ and GA₄in leaves and rapidly induced the expression of GID11 and GID13. Among them,the expression level of GID11 was 76.45 times higher than that of the control group on the 3rd day after the treatment with 50 mg · L^-1 GA₃. On the 12th day after treatment with 400 mg · L^-1 GA₃,the thylakoid granule lamellae in leaves were clearer than that of chlorosis leaves,with fewer fractured lamellae. The results preliminarily revealed regreening mechanism of chlorosis leaves induced by GA₃in‘Whangkeumbae’.

Key words: pear, leaf regreening, Fe metabolism, GA transduction gene, qRT-PCR

CLC Number:

S661.2

JIA Bing, GUO Guoling, WANG Youyu, YE Zhenfeng, LIU Li, LIU Pu, HENG Wei, ZHU Liwu. Studies on the Regreening Mechanism of the Iron-deficiency Chlorosis Leaves induced by GA₃ in‘Whangkeumbae’（Pyrus pyrifolia Nakai）[J]. Acta Horticulturae Sinica, 2021, 48(2): 254-264.

Figures/Tables 7

References 32

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基因 Gene	引物序列 Primer sequence	基因 Gene	引物序列 Primer sequence
FER1	Forword：AGGTCTCTCTTGCCCGTCAG	GA2ox	Forword：AATCGCTCTGTTCATCC
FER1	Reverse：GCAACGTTGTCCCTGTCAAA		Reverse：GGAAATAACGCAGAAGAG
FER2	Forword：TTCTCGCCTCTGACCTCCTC	DELLA4	Forword：ACAGCATACAGACCCGTCCAT
FER2	Reverse：CGAAATAGGCGTACATGGCA	DELLA4	Reverse：TGTACCCCAACATCGCCAGAA
FER3	Forword：TGCTTTTGTTGTCCCTGTGG	GID11	Forword：AGCGGAATTCCTTGACCGGAA
FER3	Reverse：TCCTCTTTCTTCCTCGCTCG	GID11	Reverse：CACCGCTCTCTCAGGATCAACA
FRO2	Forword：TCATTGCGGTGTTGGGTTGT	GID13	Forword：TTCCGGTTGACGGGGTTTTCT
FRO2	Reverse：ATCGCTTCTTCTTTCTGTCATTGCT	GID13	Reverse：GAAGCTTCCGCCGTGGAAAAA
IRT1	Forword：GTCTGAGGAGGACGAGTGC	GID15	Forword：ATCATCGGGGGACGATTCGAT
IRT1	Reverse：TGGAAGGCTGGTATGGAAC	GID15	Reverse：TCATCCTGGTCAACGCAGTCA
FD1	Forword：GGTCAGCACCTCCTTCATCC	GID16	Forword：TCGAGCCAGAACGTGGTTGAA
FD1	Reverse：TACGTCCTCAGGGCACTCAA	GID16	Reverse：TGCATTCAGAACCATGGCAGC

基因 Gene	引物序列 Primer sequence	基因 Gene	引物序列 Primer sequence
FER1	Forword：AGGTCTCTCTTGCCCGTCAG	GA2ox	Forword：AATCGCTCTGTTCATCC
FER1	Reverse：GCAACGTTGTCCCTGTCAAA		Reverse：GGAAATAACGCAGAAGAG
FER2	Forword：TTCTCGCCTCTGACCTCCTC	DELLA4	Forword：ACAGCATACAGACCCGTCCAT
FER2	Reverse：CGAAATAGGCGTACATGGCA	DELLA4	Reverse：TGTACCCCAACATCGCCAGAA
FER3	Forword：TGCTTTTGTTGTCCCTGTGG	GID11	Forword：AGCGGAATTCCTTGACCGGAA
FER3	Reverse：TCCTCTTTCTTCCTCGCTCG	GID11	Reverse：CACCGCTCTCTCAGGATCAACA
FRO2	Forword：TCATTGCGGTGTTGGGTTGT	GID13	Forword：TTCCGGTTGACGGGGTTTTCT
FRO2	Reverse：ATCGCTTCTTCTTTCTGTCATTGCT	GID13	Reverse：GAAGCTTCCGCCGTGGAAAAA
IRT1	Forword：GTCTGAGGAGGACGAGTGC	GID15	Forword：ATCATCGGGGGACGATTCGAT
IRT1	Reverse：TGGAAGGCTGGTATGGAAC	GID15	Reverse：TCATCCTGGTCAACGCAGTCA
FD1	Forword：GGTCAGCACCTCCTTCATCC	GID16	Forword：TCGAGCCAGAACGTGGTTGAA
FD1	Reverse：TACGTCCTCAGGGCACTCAA	GID16	Reverse：TGCATTCAGAACCATGGCAGC

Studies on the Regreening Mechanism of the Iron-deficiency Chlorosis Leaves induced by GA₃ in‘Whangkeumbae’（Pyrus pyrifolia Nakai）

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