Abstract:

In order to understand and to control the wilt disease observed on Lilium brownii var. viridulum,the wilt were collected to isolate and to identify its pathogen. Combinating morphological method with molecular identification of rDNA-ITS,EF-1α,mtSSU and IGS genes,the pathogen was identified as Fusarium commune. Growth response of the pathogen to different light treatments,temperature,pH,carbon sources,and nitrogen sources were also studied. The results showed that the most suitable condition for mycelial growth of the pathogen was 25-30 ℃,pH 8.0,lactose source and peptone source;and the most suitable condition for sporulation was 24 h dark,30 ℃,pH 10.0,lactose source and peptone source. In addition,the conditions of 24 light,24 h dark and 12 h light-dark cycle were all suitable for mycelial growth. In the aspect of sensitivity,the results revealed that the inhibitory effects of the five fungicides on the pathogen had significant differences in mycelial growth. 75% trifloxystrobin·tebuconazole water dispersible granules had stronger inhibitory effect than the others leading to the 50% of maximal effect（EC₅₀）of 0.294 mg·L ^-1;30% hymexazol aqueous solution had the minimum inhibitory effect,which led to the EC₅₀ value of 39.359 mg·L^-1.

Key words: Lilium brownii var. viridulum, Fusarium commune, growth characteristics, fungicides, sensitivity