Cloning of MhMYB114-Like from Malus halliana and Its Functional Identification of Iron Deficiency Tolerance in Apple Callus

doi:10.16420/j.issn.0513-353x.2020-0208

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 127-136.doi: 10.16420/j.issn.0513-353x.2020-0208

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Cloning of MhMYB114-Like from Malus halliana and Its Functional Identification of Iron Deficiency Tolerance in Apple Callus

ZHANG Zhongxing, CHENG Li, WANG Shuangcheng, ZHANG De, LIU Bing, WANG Yanxiu^*()

College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China

Received:2020-05-06 Revised:2020-07-10 Online:2021-01-25 Published:2021-01-29
Contact: WANG Yanxiu E-mail:wangxy@gsau.edu.cn

Abstract

Abstract:

The MhMYB114-Like gene（GenBank accession number:LOC103405832）was cloned from apple rootstock Malus halliana. The gene contains a full open reading frame of 602 bp,which encodes 200 amino acids,and the protein has an isoelectric point of 9.38. The phylogenetic tree analysis showed that apple MhMYB114-Like was the closest relative to wild strawberry. Promoter cis-element prediction of apple MhMYB114-Like showed that there were drought,light,auxin,yellowing and other responsive regulatory elements. Fluorescence quantitative PCR analysis demonstrated that the expression level of MhMYB114-Like gene was greatly increased in the leaves and roots of Malus halliana under iron deficiency condition,and the expression was obviously induced by iron deficiency stress. In addition,The genetic transformation of MhMYB114-Like into apple‘Orin’callus and resistance analysis by measuring related physiological indexes showed that the overexpression of MhMYB114-Like could significantly improve the resistance of transgenic callus to iron deficiency stress. Therefore,MhMYB114-Like is involved in the response to iron deficiency and play a crucial role in resisting iron deficiency stress.

Key words: apple, MhMYB114-Like, functional identification, iron deficiency stress, gene expression

CLC Number:

S661.1

ZHANG Zhongxing, CHENG Li, WANG Shuangcheng, ZHANG De, LIU Bing, WANG Yanxiu. Cloning of MhMYB114-Like from Malus halliana and Its Functional Identification of Iron Deficiency Tolerance in Apple Callus[J]. Acta Horticulturae Sinica, 2021, 48(1): 127-136.

Figures/Tables 9

References 25

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元件名称 cis-Acting element	核心序列 Sequence	位点功能 Function of site	位置 Location
GT1CONSENSUS	GRWAAW	光响应元件cis-Acting element involved in light responsiveness	-586
DPBFCOREDCDC3	ACACNNG	脱落酸响应cis-Acting element involved in abscisic ascid responsiveness	-619
LTRECOREATCOR15	CCGAC	耐寒响应 cis-Acting element involved in resistance cold responsiveness	-1 549
CIACADIANLELHC	CAANNNNATC	生理节律响应cis-Acting element involved in circadian responsiveness	-1 203
ACGTATERD1	ACGT	黄化响应元件cis-Acting element involved in chlorisis responsiveness	-804
DOFCOREZM	AAAG	碳代谢响应元件cis-Acting element involved in carbon metabolism	+ 704
CATATGGMSAUR	CATATG	生长素响应元件cis-Acting element involved in the auxin responsiveness	+ 1 217
SREATMSD	TTATCC	糖响应元件cis-Acting element involved in sugar responsiveness	+ 946
PYRIMIDINEBOXHVEPB1	TTTTTTCC	激素调控元件cis-Acting element involved in hormone responsiveness	+ 1 877
ARR1AT	NGATT	细胞分裂素响应cis-Acting element involved in cytokinin responsiveness	+ 1 991
MYB2CONSENSUSAT	YAACKG	耐旱响应cis-Acting element involved in drought-tolerant responsiveness	+ 1 049

元件名称 cis-Acting element	核心序列 Sequence	位点功能 Function of site	位置 Location
GT1CONSENSUS	GRWAAW	光响应元件cis-Acting element involved in light responsiveness	-586
DPBFCOREDCDC3	ACACNNG	脱落酸响应cis-Acting element involved in abscisic ascid responsiveness	-619
LTRECOREATCOR15	CCGAC	耐寒响应 cis-Acting element involved in resistance cold responsiveness	-1 549
CIACADIANLELHC	CAANNNNATC	生理节律响应cis-Acting element involved in circadian responsiveness	-1 203
ACGTATERD1	ACGT	黄化响应元件cis-Acting element involved in chlorisis responsiveness	-804
DOFCOREZM	AAAG	碳代谢响应元件cis-Acting element involved in carbon metabolism	+ 704
CATATGGMSAUR	CATATG	生长素响应元件cis-Acting element involved in the auxin responsiveness	+ 1 217
SREATMSD	TTATCC	糖响应元件cis-Acting element involved in sugar responsiveness	+ 946
PYRIMIDINEBOXHVEPB1	TTTTTTCC	激素调控元件cis-Acting element involved in hormone responsiveness	+ 1 877
ARR1AT	NGATT	细胞分裂素响应cis-Acting element involved in cytokinin responsiveness	+ 1 991
MYB2CONSENSUSAT	YAACKG	耐旱响应cis-Acting element involved in drought-tolerant responsiveness	+ 1 049

Cloning of MhMYB114-Like from Malus halliana and Its Functional Identification of Iron Deficiency Tolerance in Apple Callus

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