Comparative Analysis of the Photosystem Activity of Triploid Loquats and Their Tetraploid,Diploid Parents

doi:10.16420/j.issn.0513-353x.2020-0251

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 37-48.doi: 10.16420/j.issn.0513-353x.2020-0251

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Comparative Analysis of the Photosystem Activity of Triploid Loquats and Their Tetraploid,Diploid Parents

LIU Mingxiu¹^,², WANG Hongding¹, ZHANG Yuna¹, ZHANG Bangyan¹, LIU Song¹, LI Yunjia¹, DANG Jiangbo¹, HE Qiao¹, LIANG Guolu¹, GUO Qigao¹^,^**()

¹College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
²College of Animal Science and Technology,Southwest University,Chongqing 400715,China

Received:2020-09-23 Revised:2020-12-23 Online:2021-01-25 Published:2021-01-29
Contact: GUO Qigao E-mail:qgguo@126.com

Abstract

Abstract:

In order to explore the characteristics of photosynthetic efficiency and photosystem activity of triploid loquat,the triploidy progeny F₁（2n = 3x = 51）of B431 × GZ1 and B431 × GZ23,and their corresponding parents B431（2n = 4x = 68）,GZ1 & GZ23（2n = 2x = 34）were used to determine net photosynthetic rate（P_n）,the OJIP curve,then OJIP curve was analyzed by JIP-test in March,July,December of two consecutive years（2018-2019）. The results showed that the P_n of two triploid loquats was significantly higher than that of their diploid paternal parents,respectively;there was no significant difference with that of tetraploid maternal parent in July,and the difference with tetraploid maternal parent varied with different hybridized combination in March,December. And the difference of P_n may be mainly related to non-stomatal restriction factors. The JIP-test analysis showed that triploid loquat F₁ had higher ABS/CS_m,TR_o/CS_m,ET_o/CS_m and RE_o/CS_m,PI_abs,F_v/F_m and lower DI_o/CS_m ratio than their diploid paternal parents,respectively;while there was no significant difference with that of tetraploid maternal parent. Therefore,the higher activity of the triploid photosystem is a basic reason for its higher light utilization efficiency and regulation ability.

Key words: loquat, triploid, net photosynthetic rate, photosystem, the fast chlorophyll fluorescence induction kinetics

CLC Number:

S667.3

LIU Mingxiu, WANG Hongding, ZHANG Yuna, ZHANG Bangyan, LIU Song, LI Yunjia, DANG Jiangbo, HE Qiao, LIANG Guolu, GUO Qigao. Comparative Analysis of the Photosystem Activity of Triploid Loquats and Their Tetraploid,Diploid Parents[J]. Acta Horticulturae Sinica, 2021, 48(1): 37-48.

Figures/Tables 8

References 40

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材料编号 Material	倍性 Ploidy
B431（龙泉1号Longquan 1）	四倍体 Tetraploid（2n = 4x = 68）
GZ1（贵州1号Guizhou 1）	二倍体 Diploid（2n = 2x = 34）
GZ23（贵州23号Guizhou 23）	二倍体 Diploid（2n = 2x = 34）
B431 × GZ1	三倍体 Triploid（2n = 3x = 51）
B431 × GZ23	三倍体 Triploid（2n = 3x = 51）

材料编号 Material	倍性 Ploidy
B431（龙泉1号Longquan 1）	四倍体 Tetraploid（2n = 4x = 68）
GZ1（贵州1号Guizhou 1）	二倍体 Diploid（2n = 2x = 34）
GZ23（贵州23号Guizhou 23）	二倍体 Diploid（2n = 2x = 34）
B431 × GZ1	三倍体 Triploid（2n = 3x = 51）
B431 × GZ23	三倍体 Triploid（2n = 3x = 51）

2018		2019
日期/（M-D）Date	℃	日期/（M-D）Date	℃
03-20	12 ~ 23	03-27	15 ~ 20
03-21	12 ~ 23	03-28	14 ~ 24
03-22	10 ~ 25	03-29	12 ~ 28
07-19	27 ~ 41	07-27	29 ~ 37
07-20	30 ~ 40	07-28	29 ~ 38
07-21	28 ~ 41	07-29	27 ~ 38
12-16	3 ~ 15	12-05	7 ~ 13
12-17	3 ~ 15	12-06	5 ~ 15
12-18	6 ~ 11	12-07	6 ~ 15

2018		2019
日期/（M-D）Date	℃	日期/（M-D）Date	℃
03-20	12 ~ 23	03-27	15 ~ 20
03-21	12 ~ 23	03-28	14 ~ 24
03-22	10 ~ 25	03-29	12 ~ 28
07-19	27 ~ 41	07-27	29 ~ 37
07-20	30 ~ 40	07-28	29 ~ 38
07-21	28 ~ 41	07-29	27 ~ 38
12-16	3 ~ 15	12-05	7 ~ 13
12-17	3 ~ 15	12-06	5 ~ 15
12-18	6 ~ 11	12-07	6 ~ 15

材料 Material	PI_abs		F_v/F_m		V_j		V_i	ABS/CS_m
B431	4.66 ± 0.37 b		0.81 ± 0.01 ab		0.51 ± 0.01 b		0.77 ± 0.01 a	46 340.57 ± 2 330.40 a
B431 × GZ1	6.63 ± 0.56 a		0.82 ± 0.004 a		0.49 ± 0.02 b		0.75 ± 0.01 a	44 771.71 ± 1 745.30 a
B431 × GZ23	4.44 ± 0.31 bc		0.81 ± 0.003 a		0.59 ± 0.01 a		0.77 ± 0.01 a	46 471.86 ± 1 341.40 a
GZ1	3.89 ± 0.29 bc		0.79 ± 0.01 bc		0.53 ± 0.02 ab		0.77 ± 0.01 a	33 239.83 ± 1 129.00 b
GZ23	2.98 ± 0.35 c		0.79 ± 0.001 c		0.56 ± 0.02 ab		0.77 ± 0.01 a	41 298.00 ± 1 108.8 a
材料 Material		Di_o/CS_m		TR_o/CS_m		ET_o/CS_m			RE_o/CS_m
B431		8 657.00 ± 212.33 a		37 774.14 ± 2 171.20 a		18 856.29 ± 1516.10 ab			8 867.13 ± 826.47 a
B431 × GZ1		7 871.86 ± 321.45 a		37 164.43 ± 1 510.20 a		19 353.43 ± 1204.60 a			9 011.00 ± 574.37 a
B431 × GZ23		8 632.43 ± 314.14 a		37 493.30 ± 1 115.4 a		15 833.43 ± 341.60 abc			8 316.29 ± 127.45 ab
GZ1		6 777.83 ± 112.87 b		27 081.50 ± 959.00 b		12 476.17 ± 526.00 c			6 378.33 ± 198.38 b
GZ23		8 741.14 ± 208.63 a		32 831.70 ± 951.10 ab		15 048.29 ± 719.20 bc			7 412.57 ± 342.00 ab

Comparative Analysis of the Photosystem Activity of Triploid Loquats and Their Tetraploid,Diploid Parents

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材料 Material		PI_abs	F_v/F_m		V_j		V_i		ABS/CS_m
B431		5.42 ± 0.46 a	0.81 ± 0.01 a		0.43 ± 0.01 b		0.82 ± 0.004 ab		42 355.70 ± 1 081.60 a
B431 × GZ1		5.06 ± 0.53 a	0.81 ± 0.01 a		0.43 ± 0.01 b		0.78 ± 0.01 b		46 870.00 ± 1 032.00 a
B431 × GZ23		5.78 ± 0.39 a	0.81 ± 0.003 a		0.39 ± 0.02 b		0.80 ± 0.01 ab		43 367.36 ± 1 103.90 a
GZ1		3.68 ± 0.78 ab	0.74 ± 0.04 ab		0.44 ± 0.02 b		0.77 ± 0.01 b		39 826.90 ± 2 267.10 a
GZ23		2.38 ± 0.62 b	0.69 ± 0.04 b		0.52 ± 0.02 a		0.84 ± 0.02 a		40 594.75 ± 3 360.10 a
材料 Material	Di_o/CS_m			TR_o/CS_m		ET_o/CS_m		RE_o/CS_m
B431	7 834.70 ± 197.43 b			34 521.00 ± 1 071.90 a		19 753.30 ± 678.70 ab		6 511.22 ± 182.40 a
B431 × GZ1	8 727.50 ± 236.61 b			38 142.50 ± 1 021.20 a		21 753.40 ± 791.40 a		8 328.40 ± 364.71 a
B431 × GZ23	8 312.51 ± 209.15 b			35 054.80 ± 942.30 a		21 274.46 ± 473.10 a		7 459.00 ± 301.59 a
GZ1	9 501.60 ± 529.11 ab			30 325.30 ± 2 537.90 a		17 236.10 ± 1 775.5 ab		6 578.10 ± 504.70 ab
GZ23	11 058.50 ± 607.40 a			29 536.30 ± 3 599.80 a		14 815.80 ± 2 184.9 b		4 633.50 ± 668.78 b

材料 Material	PI_abs	F_v/F_m		V_j		V_i	ABS/CS_m
B431	2.93 ± 0.65 ab	0.75 ± 0.01 a		0.56 ± 0.02 a		0.83 ± 0.01 a	40 015.83 ± 1 360.30 ab
B431 × GZ1	3.92 ± 0.84 ab	0.77 ± 0.01 a		0.50 ± 0.03 ab		0.76 ± 0.01 b	40 543.30 ± 4 97.40 a
B431 × GZ23	4.23 ± 0.83 a	0.77 ± 0.01 a		0.48 ± 0.03 ab		0.75 ± 0.01 b	40 745.86 ± 1 892.40 a
GZ1	3.83 ± 0.84 ab	0.77 ± 0.01 a		0.44 ± 0.003 b		0.72 ± 0.02 b	34 238.50 ± 2 074.60 b
GZ23	2.74 ± 0.91 b	0.76 ± 0.01 a		0.54 ± 0.03 ab		0.81 ± 0.02 a	38 549.33 ± 1 078.10 ab
材料 Material	Di_o/CS_m		TR_o/CS_m		ET_o/CS_m			RE_o/CS_m
B431	9 137.50 ± 260.03 a		31 968.50 ± 1 267.30 a		13 912.30 ± 961.90 a			6 341.17 ± 412.07 ab
B431 × GZ1	9 038.14 ± 417.55 a		31 440.00 ± 577.80 a		16 061.10 ± 1 073.00 a			7 503.57 ± 356.27 ab
B431 × GZ23	8 994.00 ± 388.00 a		31 611.14 ± 1 492.50 a		16 413.57 ± 718.70 a			8 076.29 ± 146.83 a
GZ1	7 660.50 ± 200.84 a		26 220.00 ± 2 060.20 a		14 881.50 ± 1 161.10 a			7 448.17 ± 430.58 ab
GZ23	8 650.33 ± 364.62 a		31 740.33 ± 1 650.80 a		13 564.83 ±1 360.00 a			6 272.17 ± 699.91 b

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