Genome-wide Identification and Abiotic Stress Response Analysis of MLP Family Genes in Apple

doi:10.16420/j.issn.0513-353x.2020-0276

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 1-14.doi: 10.16420/j.issn.0513-353x.2020-0276

• Research Papers • Next Articles

Genome-wide Identification and Abiotic Stress Response Analysis of MLP Family Genes in Apple

LI Xinxin¹, HOU Hongmin¹, XU Jihua², SUN Xiaohong², ZHANG Yugang¹^,^**()

¹College of Horticulture,Qingdao Agricultural University,Qingdao Key Laboratory of Genetic Development and Breeding in Horticultural Plants,Qingdao,Shandong 266109,China
²College of Life Sciences,Qingdao Agricultural University,Qingdao 266109,China

Received:2020-05-23 Revised:2020-07-02 Online:2021-01-25 Published:2021-01-29
Contact: ZHANG Yugang E-mail:ygzhang@qau.edu.cn

Abstract

Abstract:

In the apple,13 Major Latex Protein（MLP）genes MdMLP were identified,and through sequence alignment and construction of the MdMLP protein homologous model,it was found that MdMLP contained a typical Gly-rich loop region structure of Bet_v_1 proteins, which was a unique GxxxxxG structure in the MLP family. Comparison of MLP phylogeny and collinearity in different species indicated that MdMLP had similar gene structure,conserved domain and high homology with other Rosaceae MLP members. qRT-PCR analysis showed that all MdMLP genes had different levels of expression in 14 organs of apple‘Xinjiang 1’. At the same time,MdMLP genes responded to ABA,NaCl,PEG,cold（4 ℃）and heat（40 ℃）stress treatments,and the same subgroup genes showed a similar expression trend when exposed to abiotic stress. It suggested that MdMLP may interact with PRSP,SNRK1/2,bHLH and ABA-related transcriptional proteins to participate in the defense against abiotic stress in apple.

Key words: Malus × domestica, MLP genes, genome-wide identification, abiotic stress analysis

CLC Number:

S661.1

LI Xinxin, HOU Hongmin, XU Jihua, SUN Xiaohong, ZHANG Yugang. Genome-wide Identification and Abiotic Stress Response Analysis of MLP Family Genes in Apple[J]. Acta Horticulturae Sinica, 2021, 48(1): 1-14.

Figures/Tables 9

References 38

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基因名称 Gene name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Revers primer sequences
MdMLP1	TAGCGATTGTGGTAAGCTGG	GGAGATGTGGTGTGGTTTGT
MdMLP2	ACGAGAAGTGGGAGGGTAAA	GGAGATGTGGTGTGGTTTGT
MdMLP3	TAGCGATTGTGGTAAGCTGG	GGAGATGTGGTGTGGTTTGT
MdMLP4	GCACGGGAACACAAATGATG	AGACCTCAACTGCAAACTGG
MdMLP5/13	GGCGGTTGCTTATAAGGTGA	AACCTGTTCGTGTGCTTTCT
MdMLP6	CCTTCAGGTCATCCAAGGTG	TTTGGTGTGACTTGAACCGT
MdMLP7	CGAAGGTGATTGGGAGACTG	CGCTTCAACCGTCTCCTTTA
MdMLP810	TTCTAGCAAGGTGGAGACGA	CCACAAGAAGACACAACCCA
MdMLP9	GGAAACACACGGCTCTGTTA	CCGACATGATCCCGATTGTT
MdMLP11	CAAGGCTGCAACTCTGAAAG	TATTCGATCCACAGGTTGGC
MdMLP12	CCCACCTCATCCCAAACATT	GCAATGCAGGTGCTTTCATC
MdActin	ATTCAAGTATGCCTGGGTGC	CAGTCAGCCTGTGATGTTCC

基因名称 Gene name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Revers primer sequences
MdMLP1	TAGCGATTGTGGTAAGCTGG	GGAGATGTGGTGTGGTTTGT
MdMLP2	ACGAGAAGTGGGAGGGTAAA	GGAGATGTGGTGTGGTTTGT
MdMLP3	TAGCGATTGTGGTAAGCTGG	GGAGATGTGGTGTGGTTTGT
MdMLP4	GCACGGGAACACAAATGATG	AGACCTCAACTGCAAACTGG
MdMLP5/13	GGCGGTTGCTTATAAGGTGA	AACCTGTTCGTGTGCTTTCT
MdMLP6	CCTTCAGGTCATCCAAGGTG	TTTGGTGTGACTTGAACCGT
MdMLP7	CGAAGGTGATTGGGAGACTG	CGCTTCAACCGTCTCCTTTA
MdMLP810	TTCTAGCAAGGTGGAGACGA	CCACAAGAAGACACAACCCA
MdMLP9	GGAAACACACGGCTCTGTTA	CCGACATGATCCCGATTGTT
MdMLP11	CAAGGCTGCAACTCTGAAAG	TATTCGATCCACAGGTTGGC
MdMLP12	CCCACCTCATCCCAAACATT	GCAATGCAGGTGCTTTCATC
MdActin	ATTCAAGTATGCCTGGGTGC	CAGTCAGCCTGTGATGTTCC

基因名称 Gene name	基因组登录号 Gene accession No.（GDR）	编码区/bp CDS	染色体位置 Chromosome location	蛋白质 Protein
基因名称 Gene name	基因组登录号 Gene accession No.（GDR）	编码区/bp CDS	染色体位置 Chromosome location	信号肽 Signal peptide （Sec/SPI）	长度/ aa Length	分子量/ kD Molecular weight	pI	不稳定指数 Instability index	亲水性 Hydropa- thicity
MdMLP1	MD00G1063600	468	Chr00:11768127..11768939（+）	0.0011	155	17.62	5.32	27.52	-0.576
MdMLP2	MD13G1022700	498	Chr13:1627661..1629214（-）	0.0021	165	18.68	5.52	22.52	-0.444
MdMLP3	MD13G1022900	468	Chr13:1645481..1646431（-）	0.0011	155	17.62	5.32	27.52	-0.576
MdMLP4	MD13G1023000	468	Chr13:1657247..1658331（-）	0.0016	155	17.45	5.32	26.36	-0.497
MdMLP5	MD13G1088200	462	Chr13:6177671..6178671（+）	0.0006	153	16.83	5.26	22.52	-0.137
MdMLP6	MD16G1024000	474	Chr16:1729158..1730220（-）	0.0012	157	17.58	8.43	37.24	-0.258
MdMLP7	MD16G1024100	462	Chr16:1731391..1732218（-）	0.0011	153	17.27	4.88	30.74	-0.381
MdMLP8	MD16G1024300	468	Chr16:1742688..1743368（-）	0.0007	155	17.57	4.88	30.00	-0.338
MdMLP9	MD16G1024900	558	Chr16:1792284..1793136（-）	0.0022	185	21.01	6.71	28.99	-0.334
MdMLP10	MD16G1025000	420	Chr16:1802244..1802758（-）	0.0007	139	15.78	5.33	27.31	-0.394
MdMLP11	MD16G1025800	453	Chr16:1839602..1840796（-）	0.0017	150	17.05	5.47	11.39	-0.386
MdMLP12	MD16G1026200	387	Chr16:1862558..1863354（-）	0.0022	128	14.68	6.14	20.19	-0.303
MdMLP13	MD16G1088600	462	Chr16:6094690..6095895（+）	0.0006	153	16.92	4.97	23.39	-0.145

基因名称 Gene name	基因组登录号 Gene accession No.（GDR）	编码区/bp CDS	染色体位置 Chromosome location	蛋白质 Protein
基因名称 Gene name	基因组登录号 Gene accession No.（GDR）	编码区/bp CDS	染色体位置 Chromosome location	信号肽 Signal peptide （Sec/SPI）	长度/ aa Length	分子量/ kD Molecular weight	pI	不稳定指数 Instability index	亲水性 Hydropa- thicity
MdMLP1	MD00G1063600	468	Chr00:11768127..11768939（+）	0.0011	155	17.62	5.32	27.52	-0.576
MdMLP2	MD13G1022700	498	Chr13:1627661..1629214（-）	0.0021	165	18.68	5.52	22.52	-0.444
MdMLP3	MD13G1022900	468	Chr13:1645481..1646431（-）	0.0011	155	17.62	5.32	27.52	-0.576
MdMLP4	MD13G1023000	468	Chr13:1657247..1658331（-）	0.0016	155	17.45	5.32	26.36	-0.497
MdMLP5	MD13G1088200	462	Chr13:6177671..6178671（+）	0.0006	153	16.83	5.26	22.52	-0.137
MdMLP6	MD16G1024000	474	Chr16:1729158..1730220（-）	0.0012	157	17.58	8.43	37.24	-0.258
MdMLP7	MD16G1024100	462	Chr16:1731391..1732218（-）	0.0011	153	17.27	4.88	30.74	-0.381
MdMLP8	MD16G1024300	468	Chr16:1742688..1743368（-）	0.0007	155	17.57	4.88	30.00	-0.338
MdMLP9	MD16G1024900	558	Chr16:1792284..1793136（-）	0.0022	185	21.01	6.71	28.99	-0.334
MdMLP10	MD16G1025000	420	Chr16:1802244..1802758（-）	0.0007	139	15.78	5.33	27.31	-0.394
MdMLP11	MD16G1025800	453	Chr16:1839602..1840796（-）	0.0017	150	17.05	5.47	11.39	-0.386
MdMLP12	MD16G1026200	387	Chr16:1862558..1863354（-）	0.0022	128	14.68	6.14	20.19	-0.303
MdMLP13	MD16G1088600	462	Chr16:6094690..6095895（+）	0.0006	153	16.92	4.97	23.39	-0.145

Genome-wide Identification and Abiotic Stress Response Analysis of MLP Family Genes in Apple

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