Abstract: In order to reveal the functions of banana germin-like protein（GLP），the banana GLP （MaGLP）gene family was identified. Series of bioinformatics analysis was performed to analyze the MaGLPs’ structures，chromosomal distributions and cis-elements and transcription factor binding sites （TFBS）distributions in their promoter sequences and the characteristics of their encoded proteins such as physical and chemical characteristics，motifs，and phylogenetic relationship. Additionally，based on our previous RNA-seq data，the expression of the MaGLP family members in leaves treated by 4 ℃ and 45 ℃，roots treated by Fusarium oxysporum f. sp. cubense tropical race 4（FocTR4），and fruits at different mature stages under natural condition and ethylene treatment were studied. Totally，we identified 44 MaGLP family members distributing in all the banana chromosomes except chromosome 10. The coding sequence of these genes ranges from 567 bp to 2 103 bp. Most MaGLP genes contained 1 – 2 exons，and most MaGLP proteins owned signal peptide and were predicted to be located in extracellular part. Phylogenetic analysis showed that MaGLPs could be divided into 8 subfamilies，of which subfamily M was banana specific. Prediction results of cis-acting elements and TFBS showed that there were multiple plant hormone- and stress-responsive elements and seven kinds of TFBS in the promoter regions of MaGLPs. Expression analysis revealed that the expression of MaGLP members varied a lot in different organs and were regulated by various stresses. For instance，the expression of MaGLP5-1 and MaGLP9-8 was found to be responsive to various treatments. MaGLP1-2 and MaGLP9-4 expression could be inhibited by both high and low temperature treatments，while MaGLP9-6 was induced. MaGLP5-4 expression was influenced by high temperature and FocTR4. MaGLP4-4 was found to be only high temperature responsive. Our present study indicated that MaGLPs played important roles in the stress responses of banana.

Key words: banana, germin-like protein, gene family analysis, stress response, gene expression