荔枝胚败育差异表达基因cDNA 片段的克隆及序列分析

园艺学报 ›› 2004, Vol. 31 ›› Issue (1): 25-28.

荔枝胚败育差异表达基因cDNA 片段的克隆及序列分析

张以顺¹;向旭²;傅家瑞¹;黄上志¹

(¹ 中山大学生命科学学院, 广州510275; ² 广东省农业科学院果树研究所, 广州510640)

收稿日期:2003-05-22 修回日期:2003-08-25 出版日期:2004-02-25 发布日期:2004-02-25

Cloning and Sequencing of cDNA Fragments Differentially Expressed betweenAborted and Normal Development Embryo in Litchi

Zhang Yishun¹, Xiang Xu² Fu Jiarui¹, Huang Shangzhi¹

(¹Life Science School, Zhongshan University, Guangzhou 510275, China; ²Pomology Institute, Guangdong Academy of Agricultural Science, Guangzhou 510640, China)

Received:2003-05-22 Revised:2003-08-25 Online:2004-02-25 Published:2004-02-25

摘要/Abstract

摘要： 利用抑制消减杂交(Suppressive subtraction hybridization , SSH) 克隆荔枝败育胚的差异表达基因cDNA 片段。分别以荔枝‘桂味’正常发育胚为driver (驱赶子) , 败育胚为tester (检测子) , 建立差异表达cDNA 文库, 代表桂味败育胚特异表达的cDNA。经Virtual Northern 检测, 在桂味正常发育胚/ 败育胚差异表达cDNA 文库中得到3 个阳性克隆, 序列分析表明这3 个克隆对应的两个序列在荔枝中为首次报道。

关键词: 张以顺¹, 向旭², 傅家瑞¹, 黄上志¹

Abstract: The suppressive subtraction hybridization (SSH) technique was used to clone the cDNA fragments differentially expressed between aborted and normal development embryo of ‘Guiwei’varieties. One positive differential expressing cDNA library were constructed using cDNAs of normal development embryo of Guiwei as driver and the cDNAs of aborted embryo of Guiwei varieties as tester. This cDNA library indicated the differential expressing
cDNA fragments in Guiwei aborted embryo and Guiwei normal development embryo. By Virtual Northern blots, 3 positive clones were obtained. Subsequently sequencing analysis showed the 3 clones were corresponding 3 knew gene sequence and the 3 cDNA fragments were first reported in litchi varieties.

Key words: Litchi, Aborted embryo, Suppressive subtraction hybridization (SSH), Virtual Northern blot

中图分类号:

S 667.1

张以顺;向旭;傅家瑞;黄上志. 荔枝胚败育差异表达基因cDNA 片段的克隆及序列分析[J]. 园艺学报, 2004, 31(1): 25-28.

Zhang Yishun;Xiang Xu Fu Jiarui;Huang Shangzhi. Cloning and Sequencing of cDNA Fragments Differentially Expressed betweenAborted and Normal Development Embryo in Litchi[J]. ACTA HORTICULTURAE SINICA, 2004, 31(1): 25-28.

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