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园艺学报 ›› 2009, Vol. 36 ›› Issue (8): 1099-1104.

• 果树 • 上一篇    下一篇

贮藏温度对UV-C辐射葡萄果皮白藜芦醇及其糖苷含量的影响

郑先波1,2,4; 李晓东2; 吴本宏2; 李绍华3*   

  1. (1中国农业大学农学与生物技术学院,北京 100193; 2中国科学院植物研究所,北京 100093; 3中国科学院武汉植物园,武汉 430074; 4河南农业大学园艺学院,郑州 450002)
  • 收稿日期:2009-02-17 修回日期:2009-05-07 出版日期:2009-08-25 发布日期:2009-08-25
  • 通讯作者: 郑先波

Effects of Storage Temperature on Resveratrol and Its Glycosides Content in Grape Berries Skin Following UV-C Irradiation

ZHENG Xian-bo1,2,4; LI Xiao-dong2; WU Ben-hong2; LI Shao-hua3*   

  1. (1College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; 2Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 3Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China; 4College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China)
  • Received:2009-02-17 Revised:2009-05-07 Online:2009-08-25 Published:2009-08-25
  • Contact: ZHENG Xian-bo

摘要: 用剂量3.6 kJ·m-2的紫外线C(Ultraviolet C,UV-C)辐射处理‘京蜜’葡萄成熟果实,25 ℃条件下避光孵育24 h后,控制温度为25 ℃和0 ℃贮藏,贮藏后0、0.5、1、2、3、7、10、14、24、34 d取样,HPLC法分析果皮白藜芦醇(Resveratrol,Res)及其糖苷含量。结果表明:UV-C辐射后25 ℃条件下孵育24 h能显著提高反式白藜芦醇(trans-Resveratrol, trans-Res)和顺式白藜芦醇苷(cis-Piceid, cis-PD)的含量,不能提高反式白藜芦醇苷(trans Piceid,trans PD)含量。贮藏温度对UV-C辐射葡萄果皮Res及其糖苷含量具有重要影响。无论是对照果实还是UV-C辐射处理果实,果皮中均未检测到顺式白藜芦醇(cis-Resveratrol, cis-Res)。25 ℃和0 ℃贮藏条件下对照果皮(trans-Res trans-PD、cis-PD含量低,变化幅度很小,而UV-C处理果皮中变化幅度很大,绝大部分时间显著高于对照。

关键词: 葡萄, 贮藏, 温度, UV-C辐射, 白藜芦醇, 白藜芦醇苷

Abstract:

The mature fruits of post-harvest ‘Jingmi’grape were irradiated by the dosage of 3.6 kJ·m-2 ultraviolet C (UV-C) and then incubated 24 hours in dark at 25 ℃. The UV-C irradiated berries and control berries were stored at 0 ℃ and 25 ℃. The berries was sampled at 0, 0.5, 1, 2, 3, 7, 10,14, 24 and 34 d. Resveratrol and piceid content in grape berries was determined by HPLC. The results showed: Trans-resveratrol (trans-Res) and cis-Piceid (cis-PD) content in grape berries skin was significantly increased when treated by UV-C irradiation and then incubated 24 h in dark at 25 ℃, but trans-Piceid (trans-PD) content in grape berries skin was not significantly increased. Storage temperature played an important role on the level of Res and its glycosides by UV-C irradiation treatment. cis-Resveratrol (cis-Res)could not detected in skin of UV-C irradiated berries and control berries.trans-Res, trans-PD and cis-PD content of control grape berries skin was low and stable under 25 ℃ and 0 ℃, while UV-C irradiated berries skin was higher than control at mostly sampling time and changed sharply.

Key words: grape, storage temperature, UV-C irradiation, resveratrol, piceid

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