β - 氨基丁酸诱导辣椒细胞色素P450基因的克隆及其序列分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 637-643.

β - 氨基丁酸诱导辣椒细胞色素P450基因的克隆及其序列分析

戴素明^1,2 ;周程爱^1,2 ; 谢丙炎^1*
; 肖启明² ;杨宇红¹ ; 尹丽蓉^1,2

(¹中国农业科学院蔬菜花卉研究所, 北京100081; ² 湖南农业大学生物安全科技学院, 长沙410128)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper( Capsicum annuum L. ) Induced by β-aminobutyric Acid ( BABA)

DAI Su-ming^1,2 ; ZHOU Cheng-ai^1,2 ;XIE Bing-yan^1*
, XIAO Qi-ming² ;YANG Yu-hong¹ ;YIN Li-rong^1,2

( ¹ Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²College of Plant Protection, Hunan Agricultural University, Changsha 410128, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要/Abstract

摘要： 用RT2PCR和RACE方法, 从β - 氨基丁酸(β-aminobutyric acid, BABA) 诱导辣椒叶片的基
因表达体系中克隆出CYP92A的全长cDNA序列。在BABA处理后, CYP92A 基因以一种快速而短暂的方式
作出应答。生物信息学分析表明, 该基因编码一条509个氨基酸残基的多肽, 含有一段细胞色素P450保守
的血红素结合区域, 由此CYP92A被确定是细胞色素P450。通过系统进化分析, CYP92A 属于多基因家族
P450的92A亚家族。结合已报道92A亚家族成员的研究, 推测CYP92A参与植物的防御反应。通过生物信
息学分析蛋白结构, 对CYP92A催化反应特性进行了讨论。为进一步探讨BABA的诱导机理奠定了基础。

关键词: 辣椒, 细胞色素, β - 氨基丁酸, 生物信息, 防御反应

Abstract: The complete CYP92A was amp lified by RT2PCR and RACE, which expressed in pepper (Cap
sicum annuum L. ) leaf was rapidly and transiently induced by β-aminobutyric acid (BABA). The analysis of
bioinformatics showed that CYP92A gene encoded a 5092 amino acid protein which contained a conserve heme2
binding region of cytochrome P450. Therefore, it was confirmed that CYP92A is cytochrome P450. By phyloge
netic analysis, it was belived to belong to 92A sub-family of P450. According to researches of other 92A sub
family genes reported, it is speculated conferred that CYP92A may play a role in plant defence response. Based
on bioinformatics analysis of protein struction, the p roperty of CYP92A in catalytic reaction was also discussed in
this paper. This study had significance for research on indued2resistance mechanisms of BABA.

Key words: Capsicum annuum L., Cytochrome, BABA, B ioinformatics, Plant defence response

戴素明;;周程爱;;谢丙炎;肖启明;杨宇红;尹丽蓉;. β - 氨基丁酸诱导辣椒细胞色素P450基因的克隆及其序列分析[J]. 园艺学报, 2007, 34(3): 637-643.

DAI Su-ming;;ZHOU Cheng-ai;;XIE Bing-yan;XIAO Qi-ming;YANG Yu-hong;YIN Li-rong;. Molecular Cloning and Sequence Analysis of Cytochrome P450 in Pepper( Capsicum annuum L. ) Induced by β-aminobutyric Acid ( BABA)[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 637-643.

[1]	于婷婷, 李欢, 宁源生, 宋建飞, 彭璐琳, 贾竣淇, 张玮玮, 杨洪强. 苹果GRAS全基因组鉴定及其对生长素的响应分析[J]. 园艺学报, 2023, 50(2): 397-409.
[2]	罗海林, 袁雷, 翁华, 闫佳会, 郭青云, 王文清, 马新明. 蚕豆萎蔫病毒2号青海辣椒分离物的鉴定与全基因组序列克隆[J]. 园艺学报, 2023, 50(1): 161-169.
[3]	曹亚从, 张正海, 于海龙, 冯锡刚, 张宝玺, 王立浩. 螺丝椒新品种‘中椒409号’[J]. 园艺学报, 2022, 49(S2): 111-112.
[4]	曹亚从, 张正海, 于海龙, 冯锡刚, 张宝玺, 王立浩. 螺丝椒抗病新品种‘中椒209号’[J]. 园艺学报, 2022, 49(S2): 113-114.
[5]	王永平, 胡明文, 朱文超, 廖芳芳, 白立伟, 高刚. 辣椒新品种‘红辣3号’[J]. 园艺学报, 2022, 49(S2): 115-116.
[6]	刘术均, 惠成章, 赵丽丽, 孙永生, 刘爱群. 中早熟辣椒新品种‘美罗卡’[J]. 园艺学报, 2022, 49(S2): 117-118.
[7]	王飞, 李宁, 尹延旭, 高升华, 徐凯, 姚明华. 辣椒新品种‘鄂椒红元帅’[J]. 园艺学报, 2022, 49(S2): 119-120.
[8]	李宁, 尹延旭, 高升华, 徐凯, 王飞, 姚明华. 辣椒新品种‘锦绣红117’[J]. 园艺学报, 2022, 49(S2): 121-122.
[9]	方荣, 陈学军, 周坤华, 袁欣捷, 雷刚, 黄月琴. 辣椒新品种‘赣椒18号’[J]. 园艺学报, 2022, 49(S1): 75-76.
[10]	高彦龙, 吴玉霞, 张仲兴, 王双成, 张瑞, 张德, 王延秀. 苹果ELO家族基因鉴定及其在低温胁迫下的表达分析[J]. 园艺学报, 2022, 49(8): 1621-1636.
[11]	董桑婕, 葛诗蓓, 李岚, 贺丽群, 范飞军, 齐振宇, 喻景权, 周艳虹. 不同光质补光对辣椒幼苗生长、丛枝菌根共生和磷吸收的影响[J]. 园艺学报, 2022, 49(8): 1699-1712.
[12]	李颖, 王恒明, 徐小万, 徐晓美, 黄智文. 辣椒新品种‘粤椒8号’[J]. 园艺学报, 2022, 49(8): 1837-1838.
[13]	钱婕妤, 蒋玲莉, 郑钢, 陈佳红, 赖吴浩, 许梦晗, 付建新, 张超. 百日草花青素苷合成相关MYB转录因子筛选及ZeMYB9功能研究[J]. 园艺学报, 2022, 49(7): 1505-1518.
[14]	李平平, 张祥, 刘雨婷, 谢志和, 张芮豪, 赵凯, 吕俊恒, 王梓然, 文锦芬, 邹学校, 邓明华. 辣椒63份种质果皮颜色与呈色物质的关系[J]. 园艺学报, 2022, 49(7): 1589-1601.
[15]	邹学校, 朱凡. 辣椒的起源、进化与栽培历史[J]. 园艺学报, 2022, 49(6): 1371-1381.