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园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 891-894.

• 研究报告 • 上一篇    下一篇

蝴蝶兰未受精胚珠离体培养的研究

伍成厚1, 2, 3*;潘一山1;罗开梅1;叶秀粦2*;梁承邺2   

  1. (1漳州师范学院生物系, 福建漳州363000; 2中国科学院华南植物园, 广东广州510650; 3广州市园林科学研究所,广东广州510405 )
  • 收稿日期:2005-11-15 修回日期:2006-04-27 出版日期:2006-08-25 发布日期:2006-08-25

In Vitro Culture of Unfertilized Ovules in Phalaenopsis

Wu Chenghou1, 2, 3*;Pan Yishan1;Luo Kaimei1;Ye Xiulin2*;Liang Chengye2   

  1. (1Department of Biology, Zhangzhou Normal College, Zhangzhou, Fujian 363000, China; 2South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; 3Guangzhou Institute of Landscape and Garden, Guangzhou, Guangdong 510405, China)
  • Received:2005-11-15 Revised:2006-04-27 Online:2006-08-25 Published:2006-08-25

摘要: 以蝴蝶兰大孢子母细胞时期的胚珠为外植体进行了离体培养的研究。结果表明, 在VW +BA
0.2 mg·L - 1 +NAA 0.2 mg·L - 1 +蔗糖2.0% +植物凝胶0.3%中蝴蝶兰的大孢子母细胞可以发育至二核胚囊; 在VW +BA 0.2 mg·L - 1 +NAA 0.2 mg·L - 1 +蔗糖2.0% +植物凝胶0.3% +椰子汁10%中大孢子母细胞可以发育至四核胚囊或形成胚性细胞团, 这些胚性细胞团有可能发育成单倍体植株。

关键词: 蝴蝶兰, 胚珠, 胚性细胞团, 组织培养

Abstract: The effects of medium composition on the in vitro culture of unfertilized ovules of a Phalaenopsis hybrid were studied. The results showed that the megasporocytes of the hybrid were capable of undergoing meiosis to form bi-nucleate embryo sacs in vitro by ovule cultures on the medium of VW + BA 0.2 mg·L - 1 + NAA 0.2 mg·L - 1 + sucrose 2.0% + phytagel 0.3%. On the medium of VW + BA 0.2 mg·L - 1 +NAA 0.2 mg·L - 1 + sucrose 2.0% + phytagel 0.3% + coconut milk 10% , the megasporocytes would develop into 4-nucleate embryo sacs or embryogenic cell masses. These embryogenic cell masses may develop into haploid plants.

Key words: Phalaenopsis, Ovule, Embryogenic cell masses, Tissue culture