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园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 876-878.

• 研究报告 • 上一篇    下一篇

山葵试管苗快繁体系优化初探

崔翠;何凤发*;周清元;王季春;黄远新   

  1. (西南大学农学与生物科技学院, 重庆400716)
  • 收稿日期:2005-08-22 修回日期:2005-11-14 出版日期:2006-08-25 发布日期:2006-08-25

Studies on Optimizing Production of Wasabi Plantlet

Cui Cui;He Fengfa*;Zhou Qingyuan;Wang Jichun;Huang Yuanxin   

  1. (College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China)
  • Received:2005-08-22 Revised:2005-11-14 Online:2006-08-25 Published:2006-08-25

摘要: 通过最优回归设计, 利用6-BA、NAA和维生素C不同浓度配比对山葵试管苗增殖系数进行调查, 结果表明: 当6-BA、NAA、维生素C的浓度分别为1.2504、0和1.9668 mg/L时, 增殖系数可达到6.8380。壮苗比较适宜的生根培养基为1/2MS +NAA 0.01 mg/L或者1/2MS, 生根率达90%以上。未生根的壮苗试管苗可直接移栽驯化, 且生根率可达80%以上, 能进一步降低种苗的生产成本。

关键词: 山葵, 组织培养, 最优回归设计

Abstract: The study showed that the concerntration of 6-BA, NAA and Vitamin C affected plantlet propogation coefficients ofWasabi through optimization regression analysis. As a result, the suitable combination was selected, the proliferation coefficient might increase to 6.8380 when treated byMS medium with 6-BA
1.2504 mg/L and Vitamin C 1.9668 mg/L. In rooting culture, 1 /2MS medium or 1 /2MS with 0.01mg/L NAA were suitable for root development and growth, and rooting frequency reached above 90%. In acclimatization, the results showed that strong plantletswithout rootingwere fit for culture out of door with around 80% rooting frequency, therefore, production cost of wasabi seedlings decreased greatly without rooting phases.

Key words: Wasabi, Tissue culture, Regression design