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园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 863-865.

• 研究报告 • 上一篇    下一篇

洋葱RAPD遗传多样性分析

崔成日1;徐启江2;崔崇士1;李景鹏3*   

  1. (1东北农业大学园艺学院, 黑龙江哈尔滨150030; 2 东北林业大学生命科学学院, 黑龙江哈尔滨150040; 3 东北农业大学生命科学学院, 黑龙江哈尔滨150030)
  • 收稿日期:2005-10-09 修回日期:2006-04-04 出版日期:2006-08-25 发布日期:2006-08-25

An Analysis of Onion Genetic Diversity by RAPD

Cui Chengri1; Xu Qijiang2;Cui Chongshi1;Li Jingpeng3*   

  1. (1Colllege of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; 2Colllege of Life Science, Northeast Forestry University, Harbin, Heilongjiang 150040, China; 3Colllege of Life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China)
  • Received:2005-10-09 Revised:2006-04-04 Online:2006-08-25 Published:2006-08-25

摘要: 利用RAPD技术对41份洋葱种质资源的遗传多样性进行了分析。从100个随机引物中筛选出7个引物, 共扩增出64个位点, 其中多态性位点34个(53113% ) 。材料间遗传相似系数变化范围为0.694~1.000。在遗传相似系数为0.926水平上, 聚类分析结果可将供试材料分为9类, 聚类结果与形态学特征有一定对应关系。

关键词: 洋葱, RAPD, 遗传多样性, 聚类分析

Abstract: The genetic diversity of 41 onion (Allium cepa L. ) germp lasm resources were analyzed by RAPD (Random Amplified Polymorphic DNA). Results showed that 34 ( 53.13% ) polymorphic sites were detected among the total 64 sites amplified by 7 screened random primers ( among 100 primers). The RAPD-
based genetic similarity ranged from 0.694 to 1.000. UPGMA cluster analysis showed that the tested materials could be divided into nine groups at the level of genetic similarity 0.926. Dendrogram obtained using molecular markers is homologous with separated morphologic character in some degree.

Key words: Onion, RAPD, Genetic diversity, Cluster analysis