关键词: 百合, 种质资源, 限制生长法, 离体保存, 遗传稳定性

Abstract: Studies on conservation of in vitro shoots of lily (Lilium L. ) by restricting growth method and genetic stability of recovered plantlets were carried out. Results showed that 80% of in vitro shoots could be conserved in the medium supplemented with 1.0 - 3.0 mg/L ABA formore than 9 months at normal temperature of (20 ±1) ℃, 1 500 lx and 12 h /d photoperiod. MS or 1/2MS ( half strength ofmacronutrients and micronutrients) medium supplemented with 50 - 90 g/L sucrose restricted the growth of in vitro shoots and prolonged the subculture interval to over 11 months. In vitro shoots grew slowly and produced some purple bulb during conservation. The growth of in vitro shoots cultured in medium supplemented with 10 - 50 g/L mannitol or 10 - 40 mg/L trimethylammonium (CCC) was not significantly restricted. After cultured in recoveringmedium for 1 month, all conserved in vitro shoots turned to normal plantlets and showed no obvious difference in morphology, soluble protein, isoenzynic zymogram of esterase and peroxidase compared with the control.

Key words: Lily, Germplasm resources, Restricting growth method, In vitro conservation, Genetic stability