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园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 741-744.

• 研究论文 • 上一篇    下一篇

芥蓝叶和薹的硫代葡萄糖苷组分及含量

陈新娟;朱祝军*;杨静;刘永华   

  1. (浙江大学园艺系, 浙江杭州310029)
  • 收稿日期:2005-11-05 修回日期:2006-02-09 出版日期:2006-08-25 发布日期:2006-08-25

Composition and Con tent of Glucosinolates in Leaves and Bolting Stems ofChinese Kale Quantified by HPLC

Chen Xinjuan;Zhu Zhujun*;Yang Jing;Liu Yonghua   

  1. (Department of Horticulture, Zhejiang University, Hangzhou, Zhejiang 310029, China)
  • Received:2005-11-05 Revised:2006-02-09 Online:2006-08-25 Published:2006-08-25

摘要: 采用高效液相色谱法, 对芥蓝(Brassica alboglabra Bailey) 叶片和花薹硫代葡萄糖苷(简称
硫苷) 的组分与含量进行了鉴定和分析。结果表明, 叶片和花薹的硫苷组分相同, 由6 种脂肪族硫苷(3 - 甲基亚硫酰丙基硫苷、2 - 羟基- 3 - 丁烯基硫苷、2 - 丙烯基硫苷、4 - 甲基亚硫酰丁基硫苷、5 - 甲基亚硫酰戊基硫苷和3 - 丁烯基硫苷) 和4种吲哚族硫苷(4 - 羟基吲哚甲基硫苷、吲哚甲基硫苷、4 - 甲氧基吲哚甲基硫苷和1 - 甲氧基吲哚甲基硫苷) 组成, 其中3 - 甲基亚硫酰丙基硫苷、2 - 丙烯基硫苷和5 -甲基亚硫酰戊基硫苷3种组分为芥蓝中首次被检测到。花薹总硫苷的含量达到4 907.1μg·g- 1DM, 是叶片的4.57倍。叶片和花薹中, 3 - 丁烯基硫苷为主要硫苷, 分别占硫苷总含量的56.46%和60.37%; 吲哚甲基硫苷的含量其次, 分别占总含量的18.85%和11.10%; 5 - 甲基亚硫酰戊基硫苷的含量最低, 仅占总含量的0.26%和0.39%。叶片脂肪族硫苷的相对含量低于花薹, 但是其吲哚族硫苷相对含量高于花薹。

关键词: 芥蓝, 叶, 薹, 硫代葡萄糖苷

Abstract: The composition and content of glucosinolates ( GSs) in young leaves and bolting stems ofChinese kale (Brassica alboglabra Bailey) were analyzed by HPLC. Ten kinds of GSswere identified in both parts, including six aliphatic GSs ( 3-methylsulfinylpropyl GS, 2-hydroxybut-3-enyl GS, 2-propenyl GS, 4-methylsulfinylbutyl GS, 5-methylsulfinylpentyl GS and 3-butenyl GS) and four indolyl GSs (4-hydroxyindol-3-ylmethyl GS, indol-3-ylmethyl GS, 4-methoxyindol-3-ylmethyl GS and neoindol-3-ylmethyl GS). 3-methyl-
sulfinylp ropyl GS, 2-propenyl GS and 5-methylsulfinylpentyl GS were firstly detected in Chinese kale. Total content of GSs in bolting stemswas 4 907.1μg·g- 1DM, being 4.57 folds as that in leaves. The main individual GS was 3-butenyl GS, accounting for 56.46% and 60.37% of the total GSs in leaves and bolting stems, respectively. The minor individual GS was indol-3-ylmethyl GS, accounting for 18.85% and 11.10% of the total GSs in leaves and bolting stems, respectively. And 5-methylsulfinylpentyl GS was present at the
lowest amount, only accounting for 0.26% and 0.39% of the total GSs in leaves and bolting stems, respectively. The relative p roportion of aliphatic GSs in leaves was lower than in bolting stems, and that of indolyl GSs in leaves was higher than in bolting stems.

Key words: Chinese kale (Brassica alboglabra), Leave, Bolting stem, Glucosinolate