百合种间杂交受精前障碍的研究

园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 653-656.

百合种间杂交受精前障碍的研究

刘　春;穆鼎;明　军;董　玲

(中国农业科学院蔬菜花卉研究所, 北京100081)

收稿日期:2005-12-16 修回日期:2006-04-14 出版日期:2006-06-25 发布日期:2006-06-25

The Pre-fertilization Barriers Research of Lilium Interspecific Hybridization

Liu Chun;Mu Ding;Ming Jun;Dong Ling

( Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2005-12-16 Revised:2006-04-14 Online:2006-06-25 Published:2006-06-25

摘要/Abstract

摘要： 以‘山丹’ (Lilium pumilum ) 、‘西伯利亚’ ( Siberia) 、‘白狐’ (White Fox) 、‘粉完美’( Pink Perfection) 、铁亚杂种系品种‘达诺’ (Ceb Dazzle) 和‘维多利亚瀑布’ (Victoria Falls) 为试材, 对花粉的活力、萌发力、贮存条件及授粉方式进行了研究, 结果表明适宜的蔗糖浓度有利于百合花粉萌发;花粉贮存适宜温度为5℃; 不同的授粉方式影响花粉的萌发, 授粉48 h后, 花粉管停止生长, 切花柱授粉方式的花粉处于不萌发状态; 试管受精24 h后, 花粉管即可进入胚珠, 45 d果实明显膨大, 经胚培养获得杂交苗。

关键词: 百合, 受精前障碍, 花粉活力, 萌发力, 切割柱头

Abstract: Effects of pre-fertilization barriers including the viability, germination ability, preservation conditions of pollen and pollination methods on interspecific Lilium crosses was studied in L. pumilum, ‘Siberia’, ‘White Fox’, ‘Pink Perfection’, ‘Ceb Dazzle’and‘Victoria Falls’. The results showed that the
op timum concentration of sucrose contributes to the pollen germination. Two techniques of pollination were studied comparatively for pollen germination. It appeared that pollen tubes stopped growth and the pollen was not able to germinate on cut-style 48 h after pollination. The use of in vitro pollination showed that the pollen tubes can penetrate ovary into ovules 24 hours after pollination and the strong swelling of ovary happened 45 days after pollination. The hybrid seedlings were attained by embryo rescue.

Key words: Lilium, Pre-fertilization barrier, Pollen viability, Germination, Cut-style

刘　春;穆鼎;明　军;董　玲. 百合种间杂交受精前障碍的研究[J]. 园艺学报, 2006, 33(3): 653-656.

Liu Chun;Mu Ding;Ming Jun;Dong Ling. The Pre-fertilization Barriers Research of Lilium Interspecific Hybridization[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 653-656.

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