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园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 639-641.

• 研究简报 • 上一篇    下一篇

外源亚精胺对盐胁迫下黄瓜幼苗活性氧水平和抗氧化酶活性的影响

段九菊;郭世荣*;康云艳   

  1. (南京农业大学园艺学院, 江苏南京210095)
  • 收稿日期:2005-07-25 修回日期:2005-10-17 出版日期:2006-06-25 发布日期:2006-06-25

Effects of Exogenous Spermidine on Reactive Oxygen Species Levels andAntioxidant Enzymes Activities of Cucumber Seedlings under Salt Stress

Duan Jiuju;Guo Shirong*;Kang Yunyan   

  1. (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China)
  • Received:2005-07-25 Revised:2005-10-17 Online:2006-06-25 Published:2006-06-25

摘要: 在营养液栽培、盐胁迫处理下, 研究了外源亚精胺( Spd) 对黄瓜幼苗活性氧(ROS) 水平和抗氧化酶活性的影响。结果表明, 外源Spd降低了50 mmol·L - 1NaCl胁迫下幼苗叶片和根系内O2 ·产生速率、H2O2和MDA含量, 提高了SOD、POD和CAT活性; 与抗盐性较强的‘长春密刺’相比, Spd处理对抗盐性较弱的‘津春2号’效果更明显。表明外源Spd通过提高盐胁迫下植株体内抗氧化酶活性, 降低ROS水平, 而缓解NaCl对黄瓜幼苗的伤害, 提高幼苗耐盐能力。

关键词: 亚精胺, 黄瓜, 幼苗, 盐胁迫, 活性氧, 抗氧化酶

Abstract: The cucumber seedlings were investigated under salt stress, the results showed that Spd treatment decreased the H2O2 and MDA contents, and O2 ·
production rate, while the activities of superoxide dismutase ( SOD) , peroxidase ( POD) and catalase (CAT) were promoted under 50 mmol·L - 1 NaCl stress. Spd treatment in ‘Jinchun 2’ seedlings ( low salt-resistance) was more effective than the ‘Changchun Mici’ seedlings ( high salt-resistance). It indicates that exogenous Spd treatment could mitigate the injuries caused by NaCl stress and enhance salt stress resistance of cucumber seedlings through promoting antioxidant enzymes activities and keeping low ROS levels under salt stress.

Key words: Spermidine, Cucumber, Seedling, Salt stress, Reactive oxygen species, Antioxidant enzyme