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园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 635-638.

• 研究简报 • 上一篇    下一篇

利用马铃薯茎段离体筛选耐盐变异体

程智慧;李 娟;张国裕   

  1. (西北农林科技大学园艺学院, 陕西杨凌712100)
  • 收稿日期:2005-08-22 修回日期:2005-10-24 出版日期:2006-06-25 发布日期:2006-06-25

In Vitro Selection of Salt-tolerant Mutant from Potato Stem Explants

Cheng Zhihui*;Li Juan;Zhang Guoyu   

  1. (College of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China)
  • Received:2005-08-22 Revised:2005-10-24 Online:2006-06-25 Published:2006-06-25

摘要: 以4个马铃薯品种的茎段为外植体, 在含不同浓度NaCl的培养基上直接诱导耐盐愈伤组织,筛选耐盐变异体。结果表明, 不同品种的耐盐性有差异, ‘东农303’和‘鄂1号’愈伤组织诱导的最大耐盐浓度为0.8% , ‘费乌瑞它’为0.6% , ‘夏波帝’为0.4%。盐胁迫抑制愈伤组织芽和根的分化, 耐盐愈伤组织继代不宜超过8~10次。盆栽和离体鉴定表明, 筛选出的耐盐变异体保持了耐盐的特性。

关键词: 马铃薯, 耐盐变异体, 茎段外植体, 愈伤组织, 离体培养

Abstract: The stem explants of four potato cultivarswere used to induce calli and to screen salt-tolerant mutants in the medium containing different levels of NaCl. The results indicated that different cultivars showed different tolerance levels. To calli induction, the highest salt tolerant level is 018% for cv. Dongnong 303 and E1, 0.6% for cv. Favorite and 0.4% for cv. Shepody. Salt inhibited shoot and root differentiation. The salt-tolerant calli should be subcultured for notmore than 8 - 10 times. Salt tolerant tests in vivo and in vitro revealed that the mutants possessed salt tolerance.

Key words: Potato, Salt-tolerant mutant, Stem explants, Callus, Culture in vitro