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园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 629-631.

• 研究简报 • 上一篇    下一篇

甜椒胞质雄性不育系及其保持系生化特性研究

刘金兵1, 2;侯喜林1*;陈晓峰1;张静宜1;王述彬2;潘宝贵2   

  1. (1 南京农业大学作物遗传与种质创新国家重点实验室, 江苏南京210095; 2 江苏省农业科学院蔬菜研究所, 江苏南京210014)
  • 收稿日期:2005-07-11 修回日期:2005-10-28 出版日期:2006-06-25 发布日期:2006-06-25

Studies on Biochemica l Characters in Sweet Pepper ( Capsicum annuum L. )CMS Line and Its Maintainer Line

Liu Jinbing1, 2;Hou Xilin1*;Chen Xiaofeng1;Zhang Jingyi1;Wang Shubin2;Pan Baogui2   

  1. (1National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; 2 Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China)
  • Received:2005-07-11 Revised:2005-10-28 Online:2006-06-25 Published:2006-06-25

摘要: 研究了甜椒(Capsicum annuum L. ) 胞质雄性不育系(CMS) T6A和保持系T6B的可溶性糖、
可溶性蛋白质含量以及超氧化物歧化酶( SOD) 、过氧化物酶( POD) 、过氧化氢酶(CAT) 活性的变化。结果表明: 无论是在叶片中还是在花药中, 不育系可溶性糖含量、可溶性蛋白质含量、CAT活性均低于其保持系; SOD和POD活性则高于其保持系。这说明上述物质含量和酶活性变化可能与甜椒CMS T6A的不育有关。

关键词: 甜椒, 胞质雄性不育系, 保持系, 可溶性糖, 可溶性蛋白质, 酶活性

Abstract: A comparison of soluble sugar and protein content, activites of superoxide dismutase ( SOD) ,peroxidase ( POD) and catalase (CAT) between cytoplasmic male sterile line (CMS) T6A and its maintainer line T6B of sweet pepper (Capsicum annuum L. ) in different developmental stages of leaves and buds were carried out in this study. The content of soluble sugar and protein and the activity of CAT were low in both leaves and buds and the activities of SOD and POD were high in CMS than those in its maintainer line. These results suggest that the variation of soluble sugar and protein content and enzyme activities used contribute to pollen abortion in CMS T6A.

Key words: Sweet pepper, Cytoplasmic male sterile line (CMS), Maintainer line, Soluble sugar, Soluble protein, Activity of enzyme