超干燥处理对豌豆种子抗氧化系统酶及热稳定蛋白的影响

园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 523-528.

超干燥处理对豌豆种子抗氧化系统酶及热稳定蛋白的影响

吴晓亮^{1, 2};陈晓玲²;辛萍萍²;张志娥²;陶　澜¹;卢新雄^2*

(¹ 西南农业大学农学与生命科学学院, 重庆400716;² 中国农业科学院作物科学研究所, 农业部作物种质资源与生物技术重点开放实验室, 国家农作物基因资源与基因改良重大科学工程, 北京100081)

收稿日期:2005-07-20 修回日期:2005-12-07 出版日期:2006-06-25 发布日期:2006-06-25

Effect of Ultradrying on Antioxidant Enzymes and Heat-stable Protein of PeaSeeds

Wu Xiaoliang^{1, 2};Chen Xiaoling²;Xin Pingping²;Zhang Zhi'e²;Tao Lan¹;Lu Xinxiong^2*

(¹College of Agronomy and Life Science, Southwest Agricultural University, Chongqing 400716, China; ² Key Laboratory for Crop Germplasm and Biotechnology of Agricultural Ministry, Institute of Crop Science, Chinese Academy of Agricultural Sciences, The National Key Facilities for Crop Gene Resources and Genetic Improvement, Beijing 100081, China)

Received:2005-07-20 Revised:2005-12-07 Online:2006-06-25 Published:2006-06-25

摘要/Abstract

摘要： 将含水量为12.2% , 11.3% , 8% , 5% , 4% , 3%的碗豆种子置于50℃干燥箱中人工老化,定期测定老化种子生活力和活力指标, 抗氧化系统酶活性及热稳定蛋白。结果表明: 与对照相比, 含水量4%和3%超干燥种子的活力指数、根干样质量显著下降, 而含水量8% , 5%的种子活力指数、发芽率和根干样质量没有出现明显下降。POD活性和CAT活性与种子的发芽率、活力呈正相关, 当老化处理6个月后, 含水量3%和4%的POD活性和CAT活性低于对照, 但含水量5%和8%的高于对照。超干燥种子中没有发现新的热稳定蛋白谱带, 但含水量为8%和5%的种子其热稳定蛋白质的含量增加。

关键词: 豌豆, 种子, 超干贮藏, 活力, 抗氧化系统酶, 热稳定蛋白

Abstract: Abstract: Pea seeds ( Pisum sativum L inn. ) with different moisture contents (12.2% , 11.3% , 8% , 5%, 4%, 3%) were stored in drying box at 50℃. Germination percentage, vigor index, antioxidant enzymes and heat-stable protein of aged seedswere investigated. Results showed that vigor index and dry mass of root of seeds with moisture content of 4% and 3% declined significantly at 0.05 level, but germination percentage, dry mass of root and vigor index of seedswith moisture content of 8% and 5% kep t high level. Activities of peroxidase ( POD) and catalase (CAT) positively correlated with the germination percentage and vigor index. After 6 months storage, activities of POD and CAT of seedswith moisture content of 3% and 4% were lower than control, but for seeds with moisture content of 5% and 8% , they were higher than control. New heat-stable protein was not found in the ultra-dry seeds, but content of heat-stable protein increased in seeds with moisture content of 5% and 8%.

Key words: Pea, Seed, Ultradry seed storage, Vigor, Antioxidant enzymes, Heat-stable protein

吴晓亮;;陈晓玲;辛萍萍;张志娥;陶　澜;卢新雄. 超干燥处理对豌豆种子抗氧化系统酶及热稳定蛋白的影响[J]. 园艺学报, 2006, 33(3): 523-528.

Wu Xiaoliang;;Chen Xiaoling;Xin Pingping;Zhang Zhi'e;Tao Lan;Lu Xinxiong. Effect of Ultradrying on Antioxidant Enzymes and Heat-stable Protein of PeaSeeds[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 523-528.

[1]	谭杉杉, 仇亮, 段奥其, 束胜, 曾晓萍, 朱为民, 贾敏, 阎君, 刘燕花, 刘慧, 熊爱生. 不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响[J]. 园艺学报, 2022, 49(9): 1907-1921.
[2]	梁晨, 孙如意, 向锐, 孙艺萌, 师校欣, 杜国强, 王莉. 葡萄生长调控因子GRF家族基因的鉴定及表达分析[J]. 园艺学报, 2022, 49(5): 995-1007.
[3]	李黎, 冯丹丹, 潘慧, 李文艺, 邓蕾, 汪祖鹏, 钟彩虹. 猕猴桃花粉灭菌方法比较及对果实品质的影响[J]. 园艺学报, 2022, 49(4): 769-777.
[4]	杨佳慧, 吴婷, 朱俊, 杨树华, 赵鑫, 葛红, 武荣花, 贾瑞冬. 长瓣兜兰开花特性与繁育系统研究[J]. 园艺学报, 2021, 48(5): 1002-1012.
[5]	弭宝彬, 谢玲玲, 肖伟, 戴雄泽, 张竹青, 汪端华, 周火强. 冬瓜果实与种子性状的遗传规律及单果质量通径分析[J]. 园艺学报, 2021, 48(12): 2414-2426.
[6]	李恺, 张丽丽, 邵长勇, 仲崇山, 曹逼力, 史庆华, 巩彪. 亚高温下冷等离子体处理番茄种子对幼苗生长和光能利用的影响[J]. 园艺学报, 2021, 48(11): 2286-2298.
[7]	石冬梅, 李守科, 郭先锋, 强薇, 臧德奎, 王利, 朱亮庆, 孟凡志, . 文冠果新品种‘开口笑’ [J]. 园艺学报, 2020, 47(S2): 3117-3118.
[8]	陈瑶 1,2，周寒梅 1何兵 1,*，李维 1. GA3 和 IAA 组合调控华重楼种子萌发机理初探[J]. 园艺学报, 2020, 47(2): 321-333.
[9]	闫闻, 王喜庆, 贾云鹤, 付永凯, 尤海波. 西瓜小粒种子突变体表型与遗传规律分析[J]. 园艺学报, 2020, 47(11): 2161-2168.
[10]	金宁，吕剑，郁继华，颉建明，金莉，张国斌，冯致. 外源硅对PEG渗透胁迫下黄瓜种子萌发及相关基因表达的影响[J]. 园艺学报, 2020, 47(1): 41-52.
[11]	解玮佳1,2，李世峰1，瞿素萍1，彭绿春1，张露1，杨秀梅1，王继华1,*. 常绿杜鹃不同杂种群间杂交的可育性分析[J]. 园艺学报, 2019, 46(5): 910-922.
[12]	张淑辉1，王贵芳2，罗静静1，陈晓璐1，肖元松1，彭福田1,*. 桃PpSnRK1蛋白激酶对植株根系生长的影响[J]. 园艺学报, 2019, 46(11): 2099-2108.
[13]	柴阿丽1，帕提古丽2，郭威涛1，石延霞1，谢学文1，席先梅3，李宝聚1,*. 番茄细菌性斑点病菌实时荧光定量PCR检测方法的建立及应用[J]. 园艺学报, 2019, 46(1): 182-192.
[14]	张梅1,2，刘旭3，周惠龙4，李振宇1，于胜祥1,*. 绣球属29种植物的种子微形态研究[J]. 园艺学报, 2018, 45(6): 1147-1159.
[15]	王贵芳1，于雯2，罗静静2，肖元松2，赵永飞2，陈晓璐2，彭福田2,*. 平邑甜茶MhSnRK1在番茄中超表达对种子萌发及幼苗生长的影响[J]. 园艺学报, 2018, 45(6): 1185-1192.