番茄乙烯受体基因反义表达对果实成熟的影响

园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 518-522.

番茄乙烯受体基因反义表达对果实成熟的影响

王中凤^{1, 2};应铁进^1*;张　英²;鲍碧丽¹;黄晓丹¹

(¹浙江大学食品科学与营养系, 浙江杭州310029; ² 广西工学院生物与化学工程系, 广西柳州545006)

收稿日期:2005-07-29 修回日期:2006-01-06 出版日期:2006-06-25 发布日期:2006-06-25

Effect of Ethylene Receptor Genes Antisense Transformation on Fruit Ripening

Wang Zhongfeng^{1, 2};Ying Tiejin^1*;Zhang Ying²;Bao Bili¹;Huang Xiaodan¹

(¹Department of Food Science and Nutrition, Zhejiang University, Hangzhou, Zhejiang 310029, China; ²Department of Biological and Chemical Engineering, Guangxi Institute of Technology, Liuzhou, Guangxi 545006, China)

Received:2005-07-29 Revised:2006-01-06 Online:2006-06-25 Published:2006-06-25

摘要/Abstract

摘要： 以乙烯受体基因LeETR1和LeETR2的转反义基因株系ale1和ale2为材料, 对果实成熟特性进行了研究。转基因番茄果实与对照相比, 呼吸速率下降, 但乙烯释放速率增加1～4倍, 呼吸高峰和乙烯跃变高峰出现的时间没有改变, 表明果实成熟起始时间没有改变。ale1果实成熟后期着色速度减慢, 红熟果色泽a值、番茄红素和类胡萝卜素含量都显著低于对照; 但果实多聚半乳糖醛酸酶活性上升和硬度下降速度都显著高于对照。这说明LeETR1基因与果实成熟品质特征的形成可能存在比较复杂的关系。

关键词: 番茄, 乙烯受体基因, 果实成熟

Abstract: Fruit ripening characteristics of antisense transgenic lines ale1 and ale2 respectively from ethylene receptor genes LeETR1 and LeETR2 were studied. Compared to the wild type, transgenic lines disp layed that respiration rate decreased, whereas ethylene production increased 1 - 4 folds, which suggest that starting time of fruit ripening were not affected. At later stage of ale1 fruit ripening, a-value, carotenoid and lycopene contents was lower than that in wild type fruit. Although the speed of fruit coloring slow down in ale1, the polygalacturonase increasing and firmness decreasing were sped up. The results suggest that there is some intricate relation between LeETR1 and fruit ripening.

Key words: Tomato, Ethylene receptor gene, Fruit ripening

王中凤;;应铁进;张　英;鲍碧丽;黄晓丹. 番茄乙烯受体基因反义表达对果实成熟的影响[J]. 园艺学报, 2006, 33(3): 518-522.

Wang Zhongfeng;;Ying Tiejin;Zhang Ying;Bao Bili;Huang Xiaodan. Effect of Ethylene Receptor Genes Antisense Transformation on Fruit Ripening[J]. ACTA HORTICULTURAE SINICA, 2006, 33(3): 518-522.

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