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园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 511-517.

• 研究论文 • 上一篇    下一篇

桃果肉颜色、果皮茸毛和花粉育性性状的分子标记

俞明亮1;马瑞娟1;沈志军1;章 镇2   

  1. (1 江苏省农业科学院园艺研究所, 江苏南京210014; 2 南京农业大学园艺学院, 江苏南京210095)
  • 收稿日期:2005-07-24 修回日期:2005-11-18 出版日期:2006-06-25 发布日期:2006-06-25

Molecular Markers Linked to Specific Characteristics of Prunus persica(L. ) Batsch

Yu Mingliang1;Ma Ruijuan1;Shen Zhijun1;Zhang Zhen2   

  1. (1 Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China; 2College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China)
  • Received:2005-07-24 Revised:2005-11-18 Online:2006-06-25 Published:2006-06-25

摘要: 以91-42-51 ×瑞光2号的杂交后代共48个单株为试材, 采用集群分离分析法(BSA) , 对桃果实的有毛/无毛、白肉/黄肉以及无花粉/有花粉3 个性状进行了分子标记研究。研究获得SSR 标记UDP96-018 (300 bp) 与有毛性状连锁, 遗传距离4.5 cM, SSR标记UDP98-407 (680 bp) 与白肉性状连锁, 遗传距离为2.2 cM; 根据拟南芥雄性不育序列标记设计的引物扩增出的两个片段NNJ-I (600 bp ) 和NNJ-I (900 bp) 与桃无花粉性状之间的遗传距离为0 cM。这些标记的获得为桃分子标记辅助育种提供了有效的筛选手段。

关键词: 桃, 果肉颜色, 果皮茸毛, 花粉育性, 分子标记

Abstract: A population of 48 individualswas developed from cross‘91-42-51’ בRuiguang 2’, and was used to find molecular markers linked to characters as peach or nectarine, yellow or white flesh, male sterility or male fertility by bulked segregant analysis (BSA). The results showed that SSR marker UDP96-018(300 bp) was found linked to peach gene with a distance of 4.5 cM, and UDP98-407 (680 bp ) linked to white flesh gene with a distance of 2.2 cM. Marker NNJ-I ( 600 bp ) and NNJ-I ( 900 bp ) amplified by primers which were designed according to male sterility sequence of Arabidopsis thaliana were tightly linked to male sterility gene with a distance of 0 cM. Markers linked to these characters obtained in this study would provide effective tools for marker assisted selection in peach breeding.

Key words: Prunus persica, Peach, White flesh, Pollen sterility, Molecular markers